Source 1primary paper2019ChemBioChem
Toolkit/Lamprey parapinopsin (UVLamP)
Lamprey parapinopsin (UVLamP)
Also known as: Japanese lamprey parapinopsin, UVLamP
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Lamprey parapinopsin (UVLamP) is a Japanese lamprey (Lethenteron camtschaticum) parapinopsin used as a bistable, narrow-bandwidth optogenetic probe for control of the Gi/o pathway. It is activated by a millisecond UV light pulse and switched off by a millisecond blue light pulse, enabling reversible optical control of GPCR signaling.
Usefulness & Problems
Why this is useful
UVLamP is useful as a minimally invasive optogenetic switch for reversible control of Gi/o signaling with narrow-band optical inputs. Its bistable behavior allows sustained pathway activation after a brief UV pulse and on-demand deactivation with a blue pulse.
Source:
The Japanese lamprey (Lethenteron camtschaticum) parapinopsin (“UVLamP”) serves as a minimally invasive, narrow-bandwidth, bistable, next-generation optogenetic probe for controlling the Gi/o pathway.
Problem solved
UVLamP addresses the need for ultrafast, reversible, and spectrally constrained optical control of GPCR Gi/o pathways. The available evidence specifically supports its use for sustained activation triggered by millisecond UV illumination and termination by millisecond blue illumination.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
Structural CharacterizationTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The evidence indicates that UVLamP is derived from Japanese lamprey parapinopsin and is controlled with millisecond UV pulses for activation and millisecond blue pulses for deactivation. A first structural model of the dark state has been reported, but the supplied material does not specify chromophore requirements, construct architecture, expression system, or delivery method.
The supplied evidence is limited to a single source and does not provide quantitative performance metrics such as activation amplitude, kinetics beyond millisecond pulse triggering, expression requirements, or cell-type validation. The evidence also does not describe in vivo performance, spectral cross-talk measurements, or comparisons with other optogenetic GPCR tools.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Lamprey parapinopsin (UVLamP) serves as a minimally invasive, narrow-bandwidth, bistable optogenetic probe for controlling the Gi/o pathway.
The Japanese lamprey (Lethenteron camtschaticum) parapinopsin (“UVLamP”) serves as a minimally invasive, narrow-bandwidth, bistable, next-generation optogenetic probe for controlling the Gi/o pathway.
The first structural model of parapinopsin in the dark state reveals novel interaction partners relevant to mechanisms of opsin bistability.
The first structural model of parapinopsin in the dark state reveals novel interaction partners shedding light on the mechanisms responsible for opsin bistability.
A millisecond UV light pulse allows sustained pathway activation by UVLamP, and a millisecond blue light pulse can switch that activation off on demand.
A millisecond UV light pulse allows for sustained pathway activation that can be switched off with a millisecond blue light pulse on demand.
activation pulse duration milliseconddeactivation pulse duration millisecond
Approval Evidence
1 source3 linked approval claimsfirst-pass slug lamprey-parapinopsin-uvlamp
The Japanese lamprey (Lethenteron camtschaticum) parapinopsin (“UVLamP”) serves as a minimally invasive, narrow-bandwidth, bistable, next-generation optogenetic probe for controlling the Gi/o pathway.
Source:
functional rolesupports
Lamprey parapinopsin (UVLamP) serves as a minimally invasive, narrow-bandwidth, bistable optogenetic probe for controlling the Gi/o pathway.
The Japanese lamprey (Lethenteron camtschaticum) parapinopsin (“UVLamP”) serves as a minimally invasive, narrow-bandwidth, bistable, next-generation optogenetic probe for controlling the Gi/o pathway.
Source:
structural insightsupports
The first structural model of parapinopsin in the dark state reveals novel interaction partners relevant to mechanisms of opsin bistability.
The first structural model of parapinopsin in the dark state reveals novel interaction partners shedding light on the mechanisms responsible for opsin bistability.
Source:
switching behaviorsupports
A millisecond UV light pulse allows sustained pathway activation by UVLamP, and a millisecond blue light pulse can switch that activation off on demand.
A millisecond UV light pulse allows for sustained pathway activation that can be switched off with a millisecond blue light pulse on demand.
Source:
Comparisons
Source-backed strengths
Reported strengths include bistability, narrow-bandwidth optical control, and minimally invasive operation as an optogenetic probe for the Gi/o pathway. The source also states that millisecond UV pulses produce sustained activation and that millisecond blue pulses switch signaling off on demand.
Ranked Citations
- 1.