Source 1review2023Frontiers in Molecular Biosciences
Toolkit/lateral flow technology
lateral flow technology
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Lateral flow technology is a signal transformation format used within CRISPR-Cas pathogen nucleic acid diagnostic platforms. In the supplied evidence, it functions alongside Cas protein-based sequence recognition and cleavage and with signal amplification approaches for rapid molecular diagnosis.
Usefulness & Problems
Why this is useful
This format is useful as a readout modality that converts CRISPR-Cas nucleic acid detection events into a diagnostic signal. The evidence specifically places lateral flow technology among signal transformation methods used for rapid pathogen nucleic acid detection.
Source:
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Problem solved
It helps solve the problem of transforming Cas-mediated target recognition and cleavage into an assay output suitable for pathogen molecular diagnosis. The supplied evidence does not provide further detail on the exact analytical bottleneck or workflow step addressed beyond signal transformation in diagnostic platforms.
Source:
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Problem links
Lateral flow is a directly relevant point-of-care readout format for rapid molecular diagnostics and is well aligned with low-cost, portable use in low-resource settings. The supplied evidence specifically places it within CRISPR pathogen detection workflows, which could support simple field-deployable tests.
As a readout format within CRISPR-based nucleic acid diagnostics, lateral flow could help convert molecular detection into fast, simple surveillance tests. This is relevant to the gap's need for rapidly adaptable rapid test platforms, though not to untargeted discovery or attribution.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
sequence-specific nucleic acid recognition and cleavage by cas proteinssignal transformationtrans-cleavage activity of cas proteinsTechniques
Functional AssayTarget processes
diagnosticImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The evidence indicates implementation in combination with Cas proteins and signal amplification technologies in pathogen nucleic acid detection workflows. No construct design, reporter chemistry, sample type, cofactor requirements, or device-format details are provided in the supplied material.
The supplied evidence does not describe assay sensitivity, specificity, limit of detection, time to result, or compatibility with particular Cas effectors. It also does not provide independent validation data or direct mechanistic detail for the lateral flow format itself beyond its classification as a signal transformation technology.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Cas effector proteins recognize and cut specific DNA or RNA sequences, which underlies their use in molecular diagnosis.
Cas effector proteins have endonucleases, and become a hotspot in the field of molecular diagnosis because they recognize and cut specific DNA or RNA sequences.
The review summarizes rapid pathogen nucleic acid detection technologies based on the trans-cleavage activity of Cas proteins.
This paper introduces the biological mechanism and classification of CRISPR-Cas technology, summarizes the existing rapid detection technology for pathogen nucleic acid based on the trans cleavage activity of Cas
Approval Evidence
1 source1 linked approval claimfirst-pass slug lateral-flow-technology
in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Source:
application summarysupports
Researchers have developed diagnostic platforms for pathogen nucleic acid detection using Cas proteins together with signal amplification and signal transformation technologies.
Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.)
Source:
Comparisons
Source-backed strengths
The evidence supports that lateral flow technology is incorporated into CRISPR-Cas diagnostic platforms together with signal amplification and Cas-based nucleic acid recognition. Its cited strength is its role in rapid pathogen nucleic acid diagnosis, but no quantitative performance metrics are provided.
Compared with magnetic nanoparticle biosensors
lateral flow technology and magnetic nanoparticle biosensors address a similar problem space because they share diagnostic.
Shared frame: same top-level item type; shared target processes: diagnostic
Compared with magnetic resonance elastography
lateral flow technology and magnetic resonance elastography address a similar problem space because they share diagnostic.
Shared frame: same top-level item type; shared target processes: diagnostic
Compared with qRT-PCR
lateral flow technology and qRT-PCR address a similar problem space because they share diagnostic.
Shared frame: same top-level item type; shared target processes: diagnostic
Relative tradeoffs: appears more independently replicated.
Ranked Citations
- 1.