Source 1primary paper2021Journal of Visualized Experiments
Toolkit/LED illumination system
LED illumination system
Also known as: illumination system, low-cost and versatile LED illumination system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The LED illumination system is a low-cost, versatile, and customizable light-delivery harness for optogenetic experiments. It provides programmable illumination with automated precise timing and pulsing through an open-source user interface running on a personal computer or inexpensive tablet.
Usefulness & Problems
Why this is useful
This system is useful as an accessible illumination platform for optogenetic experiments, particularly where low cost and customization across different available optogenetic tools are important. The protocol states that no prior expertise in electronics is required to build and use it, which lowers the barrier to implementing controlled light stimulation.
Source:
This system is configurable for manual or computer control with adjustable LED intensity.
Source:
This automation makes the system useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities that span large time scales.
Problem solved
It addresses the practical problem of delivering programmable light stimulation for optogenetic experiments without requiring specialized electronics expertise. It also addresses the need for automated timing and pulsed illumination control using commonly available computing hardware.
Source:
This automation makes the system useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities that span large time scales.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.
Techniques
No technique tags yet.
Target processes
signalingInput: Light
Implementation Constraints
The system is described as an LED-based illumination setup built according to a published protocol for optogenetic experiments. Practical implementation features supported by the evidence include an open-source user interface and operation from a personal computer or inexpensive tablet; the evidence does not specify LED wavelengths, power electronics, or construct-level integration details.
The supplied evidence does not report quantitative optical performance, wavelength range, irradiance, spatial uniformity, or validation in specific biological assays. Independent replication and comparative benchmarking against other illumination platforms are not provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
No prior expertise in electronics is required to build and use the illumination system for optogenetic experiments in this protocol.
For this protocol, no prior expertise in electronics is required to build all the parts needed or to use the illumination system to perform optogenetic experiments.
No prior expertise in electronics is required to build and use the illumination system for optogenetic experiments in this protocol.
For this protocol, no prior expertise in electronics is required to build all the parts needed or to use the illumination system to perform optogenetic experiments.
No prior expertise in electronics is required to build and use the illumination system for optogenetic experiments in this protocol.
For this protocol, no prior expertise in electronics is required to build all the parts needed or to use the illumination system to perform optogenetic experiments.
No prior expertise in electronics is required to build and use the illumination system for optogenetic experiments in this protocol.
For this protocol, no prior expertise in electronics is required to build all the parts needed or to use the illumination system to perform optogenetic experiments.
No prior expertise in electronics is required to build and use the illumination system for optogenetic experiments in this protocol.
For this protocol, no prior expertise in electronics is required to build all the parts needed or to use the illumination system to perform optogenetic experiments.
No prior expertise in electronics is required to build and use the illumination system for optogenetic experiments in this protocol.
For this protocol, no prior expertise in electronics is required to build all the parts needed or to use the illumination system to perform optogenetic experiments.
No prior expertise in electronics is required to build and use the illumination system for optogenetic experiments in this protocol.
For this protocol, no prior expertise in electronics is required to build all the parts needed or to use the illumination system to perform optogenetic experiments.
The open-source user interface enables automated precise timing and pulsing of light on a personal computer or inexpensive tablet.
Using our open-source user interface, users can automate precise timing and pulsing of light on a personal computer (PC) or an inexpensive tablet.
The open-source user interface enables automated precise timing and pulsing of light on a personal computer or inexpensive tablet.
Using our open-source user interface, users can automate precise timing and pulsing of light on a personal computer (PC) or an inexpensive tablet.
The open-source user interface enables automated precise timing and pulsing of light on a personal computer or inexpensive tablet.
Using our open-source user interface, users can automate precise timing and pulsing of light on a personal computer (PC) or an inexpensive tablet.
The open-source user interface enables automated precise timing and pulsing of light on a personal computer or inexpensive tablet.
Using our open-source user interface, users can automate precise timing and pulsing of light on a personal computer (PC) or an inexpensive tablet.
The open-source user interface enables automated precise timing and pulsing of light on a personal computer or inexpensive tablet.
Using our open-source user interface, users can automate precise timing and pulsing of light on a personal computer (PC) or an inexpensive tablet.
The open-source user interface enables automated precise timing and pulsing of light on a personal computer or inexpensive tablet.
Using our open-source user interface, users can automate precise timing and pulsing of light on a personal computer (PC) or an inexpensive tablet.
The open-source user interface enables automated precise timing and pulsing of light on a personal computer or inexpensive tablet.
Using our open-source user interface, users can automate precise timing and pulsing of light on a personal computer (PC) or an inexpensive tablet.
The illumination system can be configured for manual or computer control with adjustable LED intensity.
This system is configurable for manual or computer control with adjustable LED intensity.
The illumination system can be configured for manual or computer control with adjustable LED intensity.
This system is configurable for manual or computer control with adjustable LED intensity.
The illumination system can be configured for manual or computer control with adjustable LED intensity.
This system is configurable for manual or computer control with adjustable LED intensity.
The illumination system can be configured for manual or computer control with adjustable LED intensity.
This system is configurable for manual or computer control with adjustable LED intensity.
The illumination system can be configured for manual or computer control with adjustable LED intensity.
This system is configurable for manual or computer control with adjustable LED intensity.
The illumination system can be configured for manual or computer control with adjustable LED intensity.
This system is configurable for manual or computer control with adjustable LED intensity.
The illumination system can be configured for manual or computer control with adjustable LED intensity.
This system is configurable for manual or computer control with adjustable LED intensity.
The paper describes a low-cost, versatile LED illumination system for optogenetic experiments that is customizable for different optogenetic tools.
Here we detail how to build a low-cost and versatile LED illumination system that is easily customizable for different available optogenetic tools.
The paper describes a low-cost, versatile LED illumination system for optogenetic experiments that is customizable for different optogenetic tools.
Here we detail how to build a low-cost and versatile LED illumination system that is easily customizable for different available optogenetic tools.
The paper describes a low-cost, versatile LED illumination system for optogenetic experiments that is customizable for different optogenetic tools.
Here we detail how to build a low-cost and versatile LED illumination system that is easily customizable for different available optogenetic tools.
The paper describes a low-cost, versatile LED illumination system for optogenetic experiments that is customizable for different optogenetic tools.
Here we detail how to build a low-cost and versatile LED illumination system that is easily customizable for different available optogenetic tools.
The paper describes a low-cost, versatile LED illumination system for optogenetic experiments that is customizable for different optogenetic tools.
Here we detail how to build a low-cost and versatile LED illumination system that is easily customizable for different available optogenetic tools.
The paper describes a low-cost, versatile LED illumination system for optogenetic experiments that is customizable for different optogenetic tools.
Here we detail how to build a low-cost and versatile LED illumination system that is easily customizable for different available optogenetic tools.
The paper describes a low-cost, versatile LED illumination system for optogenetic experiments that is customizable for different optogenetic tools.
Here we detail how to build a low-cost and versatile LED illumination system that is easily customizable for different available optogenetic tools.
The automated illumination system is useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities across large time scales.
This automation makes the system useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities that span large time scales.
The automated illumination system is useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities across large time scales.
This automation makes the system useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities that span large time scales.
The automated illumination system is useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities across large time scales.
This automation makes the system useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities that span large time scales.
The automated illumination system is useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities across large time scales.
This automation makes the system useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities that span large time scales.
The automated illumination system is useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities across large time scales.
This automation makes the system useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities that span large time scales.
The automated illumination system is useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities across large time scales.
This automation makes the system useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities that span large time scales.
The automated illumination system is useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities across large time scales.
This automation makes the system useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities that span large time scales.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug led-illumination-system
Here we detail how to build a low-cost and versatile LED illumination system that is easily customizable for different available optogenetic tools.
Source:
accessibilitysupports
No prior expertise in electronics is required to build and use the illumination system for optogenetic experiments in this protocol.
For this protocol, no prior expertise in electronics is required to build all the parts needed or to use the illumination system to perform optogenetic experiments.
Source:
automation capabilitysupports
The open-source user interface enables automated precise timing and pulsing of light on a personal computer or inexpensive tablet.
Using our open-source user interface, users can automate precise timing and pulsing of light on a personal computer (PC) or an inexpensive tablet.
Source:
capabilitysupports
The illumination system can be configured for manual or computer control with adjustable LED intensity.
This system is configurable for manual or computer control with adjustable LED intensity.
Source:
protocol descriptionsupports
The paper describes a low-cost, versatile LED illumination system for optogenetic experiments that is customizable for different optogenetic tools.
Here we detail how to build a low-cost and versatile LED illumination system that is easily customizable for different available optogenetic tools.
Source:
use casesupports
The automated illumination system is useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities across large time scales.
This automation makes the system useful for experiments that use LEDs to control genes, signaling pathways, and other cellular activities that span large time scales.
Source:
Comparisons
Source-backed strengths
Reported strengths are low cost, versatility, and easy customization for different available optogenetic tools. The open-source user interface enables automated precise timing and pulsing of light on a personal computer or inexpensive tablet, supporting controlled illumination paradigms.
Ranked Citations
- 1.