Source 1primary paper2022
Toolkit/Light Activated BioID
Light Activated BioID
Also known as: LAB
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Light Activated BioID (LAB) is an optically controlled proximity-labeling system in which the two halves of split-TurboID are fused to the photodimerizing proteins CRY2 and CIB1. Blue light induces CRY2–CIB1 association, reconstituting split-TurboID and enabling proximity-dependent biotinylation of nearby proteins.
Usefulness & Problems
Why this is useful
LAB provides light-gated control over proximity labeling, allowing biotinylation to be restricted to illuminated conditions rather than constitutively active labeling. In the cited study, it was used to map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
Source:
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Source:
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
Source:
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
Problem solved
LAB addresses the problem of background and false-positive labeling associated with conventional proximity-labeling enzymes such as TurboID. By coupling split-TurboID reconstitution to a photodimeric switch, it enables conditional activation of labeling in response to light.
Source:
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
Heterodimerizationlight-induced heterodimerizationproximity-dependent biotinylationsplit-enzyme reconstitutionTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
LAB is implemented by fusing the two halves of split-TurboID to CRY2 and CIB1, creating a multi-component construct that depends on photodimerization for activity. The available evidence indicates blue-light activation, but does not specify construct orientation, linker design, expression context, or biotin supplementation requirements.
The supplied evidence supports the construct architecture and one benchmark application, but provides limited detail on kinetics, labeling radius, illumination parameters, or performance across multiple targets and cell types. Independent replication is not established from the provided sources.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMammalian Cell Linemechanistic demo
Inferred from claim claim_3 during normalization. Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation. Derived from claim claim_3. Quoted text: upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
successMammalian Cell Linemechanistic demo
Inferred from claim claim_3 during normalization. Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation. Derived from claim claim_3. Quoted text: upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
successMammalian Cell Linemechanistic demo
Inferred from claim claim_3 during normalization. Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation. Derived from claim claim_3. Quoted text: upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
successMammalian Cell Linemechanistic demo
Inferred from claim claim_3 during normalization. Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation. Derived from claim claim_3. Quoted text: upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
successMammalian Cell Linemechanistic demo
Inferred from claim claim_3 during normalization. Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation. Derived from claim claim_3. Quoted text: upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
successMammalian Cell Linemechanistic demo
Inferred from claim claim_3 during normalization. Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation. Derived from claim claim_3. Quoted text: upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
successMammalian Cell Lineapplication demo
proteome measurement
Inferred from claim claim_5 during normalization. LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID. Derived from claim claim_5. Quoted text: We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
Source:
successMammalian Cell Lineapplication demo
proteome measurement
Inferred from claim claim_5 during normalization. LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID. Derived from claim claim_5. Quoted text: We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
Source:
successMammalian Cell Lineapplication demo
proteome measurement
Inferred from claim claim_5 during normalization. LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID. Derived from claim claim_5. Quoted text: We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
Source:
successMammalian Cell Lineapplication demo
proteome measurement
Inferred from claim claim_5 during normalization. LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID. Derived from claim claim_5. Quoted text: We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
Source:
successMammalian Cell Lineapplication demo
proteome measurement
Inferred from claim claim_5 during normalization. LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID. Derived from claim claim_5. Quoted text: We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
Source:
successMammalian Cell Lineapplication demo
proteome measurement
Inferred from claim claim_5 during normalization. LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID. Derived from claim claim_5. Quoted text: We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
Source:
Supporting Sources
Source 2primary paper2023Journal of Cell Science
Ranked Claims
LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Light-activated BioID is a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Light-activated BioID is a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Light-activated BioID is a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Light-activated BioID is a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Light-activated BioID is a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Light-activated BioID is a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
Approval Evidence
2 sources10 linked approval claimsfirst-pass slug light-activated-bioid
Our technology, called light-activated BioID (LAB)
Source:
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
Source:
benchmark comparisonsupports
LAB maps E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
We show that LAB can map E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
Source:
construct architecturesupports
LAB fuses the two halves of split-TurboID to the photodimeric proteins CRY2 and CIB1.
Our technology, called light-activated BioID (LAB), fuses the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
Source:
mechanism of actionsupports
Upon blue light illumination, CRY2 and CIB1 dimerize, reconstitute split-TurboID, and initiate biotinylation.
upon illumination with blue light, CRY2 and CIB1 dimerize, reconstitute split-TurboID and initiate biotinylation
Source:
reversibilitysupports
Turning off the light causes CRY2 and CIB1 to dissociate and halts biotinylation.
Turning off the light leads to the dissociation of CRY2 and CIB1 and halts biotinylation.
Source:
tool descriptionsupports
Light-activated BioID is a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Here, we present a light-activated proximity labeling technology for mapping protein-protein interactions at the cell membrane with high accuracy and precision.
Source:
application performancesupports
LAB can identify known binding partners of proteins while reducing background labeling and false positives.
We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
Source:
compositionsupports
LAB is generated by fusing split-TurboID halves to the photodimeric proteins CRY2 and CIB1.
Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1.
Source:
control of activitysupports
Turning off light halts the biotinylation reaction in the LAB system.
Turning off the light halts the biotinylation reaction.
Source:
mechanismsupports
Blue light exposure causes CRY2 and CIB1 to dimerize, reconstitute split-TurboID, and biotinylate proximate proteins in the LAB system.
upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins
Source:
tool descriptionsupports
LAB is a light-activated proximity labeling technology with high spatial and temporal resolution.
Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling.
Source:
Comparisons
Source-backed strengths
The reported architecture directly links optical input to split-enzyme reconstitution through CRY2 and CIB1, providing an externally controllable proximity-labeling system. In the benchmark described, LAB identified E-cadherin-binding partners with higher accuracy and significantly fewer false positives than TurboID.
Ranked Citations
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