Source 1primary paper2020Angewandte Chemie International Edition
Toolkit/light-controlled CRISPR/dCAS9 transactivation system
light-controlled CRISPR/dCAS9 transactivation system
Also known as: variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The light-controlled CRISPR/dCas9 transactivation system is a multi-component optogenetic transcriptional activator that couples CRISPR/dCas9-mediated gene activation to a light-responsive PAL–RNA aptamer interaction. It enables reversible optical control of transactivation and was described as a variation of the CRISPR/dCas9 system for light-controlled activation of gene expression.
Usefulness & Problems
Why this is useful
This platform is useful for optically controlling gene expression within a CRISPR/dCas9 framework while adding modular building blocks for synthetic biological circuit design. The reported design also reduces the coding space required for genetic manipulation, which may simplify construct payloads relative to larger multi-protein optogenetic systems.
Source:
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
Problem solved
It addresses the problem of making CRISPR/dCas9-mediated transcriptional activation responsive to light rather than constitutively active. The cited work specifically positions the system as a reversible transactivation platform with low dark-state activity.
Problem links
Need controllable genome or transcript editing
DerivedThis tool is a light-controlled CRISPR/dCas9 transactivation system for activating gene expression. It is built on the interaction of the light-responsive protein PAL with an RNA aptamer to create reversible optical control of CRISPR/dCas9-mediated transcriptional activation.
Need precise spatiotemporal control with light input
DerivedThis tool is a light-controlled CRISPR/dCas9 transactivation system for activating gene expression. It is built on the interaction of the light-responsive protein PAL with an RNA aptamer to create reversible optical control of CRISPR/dCas9-mediated transcriptional activation.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
crispr/dcas9-mediated transcriptional activationcrispr/dcas9-mediated transcriptional activationlight-controlled rna-protein interactionlight-controlled rna-protein interactionreversible transactivationreversible transactivationTechniques
Computational DesignTarget processes
editingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: actuatoroperating role: regulatorswitch architecture: multi component
Implementation is based on combining CRISPR/dCas9 transactivation with the light-responsive protein PAL and an RNA aptamer, indicating a multi-component construct design. The supplied evidence does not provide practical details on expression systems, cofactors, delivery method, or guide RNA/aptamer configuration.
The provided evidence does not specify the exact illumination wavelength, host organism, target genes, activation domain architecture, or quantitative fold-change values. Independent replication and validation across multiple biological contexts are not documented in the supplied material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug light-controlled-crispr-dcas9-transactivation-system
we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression
Source:
applicabilitysupports
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
Source:
design advantagesupports
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
Source:
performancesupports
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
Source:
tool descriptionsupports
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
Source:
Comparisons
Source-backed strengths
Reported strengths include a strong on-switch and almost no residual activity in the dark, indicating high dynamic separation between illuminated and non-illuminated states. The source also describes the platform as broadly applicable and modular for synthetic circuit design.
Source:
provides a strong on-switch with almost no residual activity in the dark
Compared with nanoCRISPR
light-controlled CRISPR/dCAS9 transactivation system and nanoCRISPR address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; same primary input modality: light
light-controlled CRISPR/dCAS9 transactivation system and optogenetic epigenetic editing toolbox for Ascl1 promoter targeting address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; same primary input modality: light
Compared with RNA aptamer
light-controlled CRISPR/dCAS9 transactivation system and RNA aptamer address a similar problem space because they share editing.
Shared frame: shared target processes: editing; shared mechanisms: reversible transactivation; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice.
Ranked Citations
- 1.