Toolkit/light-emitting diode illumination

light-emitting diode illumination

Delivery Strategy·Research·Since 2020

Also known as: illumination with a light-emitting diode, LED, light-emitting diode

Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

Light-emitting diode illumination is a light-delivery harness used with a photoactivatable CRISPR-Cas9 system to provide optical input for temporally and spatially controlled genome editing in mice. In the cited in vivo study, it was applied in the context of embryo implantation and reproductive function.

Usefulness & Problems

Why this is useful

This illumination modality is useful because it supplies the optical trigger required for spatiotemporal control of a photoactivatable CRISPR-Cas9 system in vivo. The cited study indicates utility for dissecting molecular mechanisms associated with embryo implantation and for temporally controlling reproductive functions in mice.

Source:

This system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation

Source:

and provides a therapeutic strategy for temporal control of reproductive functions in vivo

Problem solved

It helps solve the problem of delivering light to activate a photoactivatable CRISPR-Cas9 system with temporal and spatial specificity in vivo. In the reported mouse application, this enabled LIF knockdown that spatiotemporally disrupted fertility.

Source:

This system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation

Source:

and provides a therapeutic strategy for temporal control of reproductive functions in vivo

Taxonomy & Function

Primary hierarchy

Mechanism Branch

Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.

Techniques

No technique tags yet.

Target processes

editingrecombination

Input: Light

Implementation Constraints

The extraction evidence identifies the modality only as "illumination with a light-emitting diode." It is implemented as an optical input paired with a photoactivatable CRISPR-Cas9 system in mice, but the provided evidence does not specify construct design, delivery route, or device parameters.

The supplied evidence does not report illumination wavelength, intensity, exposure regimen, hardware configuration, or tissue penetration characteristics. Validation is limited to a single cited study in mice and only in the context of a photoactivatable CRISPR-Cas9 reproductive biology application.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Observations

successMouseapplication demomouse

Inferred from claim c1 during normalization. In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility. Derived from claim c1. Quoted text: Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.

Source:

successMouseapplication demomouse

Inferred from claim c1 during normalization. In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility. Derived from claim c1. Quoted text: Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.

Source:

successMouseapplication demomouse

Inferred from claim c1 during normalization. In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility. Derived from claim c1. Quoted text: Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.

Source:

successMouseapplication demomouse

Inferred from claim c1 during normalization. In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility. Derived from claim c1. Quoted text: Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.

Source:

successMouseapplication demomouse

Inferred from claim c1 during normalization. In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility. Derived from claim c1. Quoted text: Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.

Source:

successMouseapplication demomouse

Inferred from claim c1 during normalization. In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility. Derived from claim c1. Quoted text: Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.

Source:

successMouseapplication demomouse

Inferred from claim c1 during normalization. In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility. Derived from claim c1. Quoted text: Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.

Source:

Supporting Sources

Ranked Claims

Claim 1application potentialsupports2020Source 2needs review

The photoactivatable CRISPR-Cas9 system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation.

This system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation
Claim 2application potentialsupports2020Source 2needs review

The photoactivatable CRISPR-Cas9 system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation.

This system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation
Claim 3application potentialsupports2020Source 2needs review

The photoactivatable CRISPR-Cas9 system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation.

This system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation
Claim 4application potentialsupports2020Source 2needs review

The photoactivatable CRISPR-Cas9 system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation.

This system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation
Claim 5application potentialsupports2020Source 2needs review

The photoactivatable CRISPR-Cas9 system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation.

This system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation
Claim 6application potentialsupports2020Source 2needs review

The photoactivatable CRISPR-Cas9 system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation.

This system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation
Claim 7application potentialsupports2020Source 2needs review

The photoactivatable CRISPR-Cas9 system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation.

This system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation
Claim 8gene knockdown effectsupports2020Source 2needs review

In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility.

Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.
Claim 9gene knockdown effectsupports2020Source 2needs review

In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility.

Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.
Claim 10gene knockdown effectsupports2020Source 2needs review

In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility.

Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.
Claim 11gene knockdown effectsupports2020Source 2needs review

In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility.

Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.
Claim 12gene knockdown effectsupports2020Source 2needs review

In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility.

Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.
Claim 13gene knockdown effectsupports2020Source 2needs review

In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility.

Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.
Claim 14gene knockdown effectsupports2020Source 2needs review

In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility.

Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.
Claim 15mitigation summarysupports2020Source 1needs review

Reasonable use of lighting technology, including restricting illumination to essential areas and hours, directing lighting to avoid wasteful radiation, and selecting spectral emissions, is discussed as a way to reduce impacts on circadian clocks.

The article concludes by debating how these anthropogenic changes could be mitigated by more reasonable use of available technology - for example by restricting illumination to more essential areas and hours, directing lighting to avoid wasteful radiation and selecting spectral emissions, to reduce impact on circadian clocks.
Claim 16technology impact summarymixed2020Source 1needs review

The introduction of light-emitting diode technology may either exacerbate or in some cases reduce increasing light pollution.

The review addresses how the ubiquitous introduction of light-emitting diode technology may exacerbate, or in some cases reduce, the generalized ever-increasing light pollution.
Claim 17therapeutic potentialsupports2020Source 2needs review

The photoactivatable CRISPR-Cas9 system provides a therapeutic strategy for temporal control of reproductive functions in vivo.

and provides a therapeutic strategy for temporal control of reproductive functions in vivo
Claim 18therapeutic potentialsupports2020Source 2needs review

The photoactivatable CRISPR-Cas9 system provides a therapeutic strategy for temporal control of reproductive functions in vivo.

and provides a therapeutic strategy for temporal control of reproductive functions in vivo
Claim 19therapeutic potentialsupports2020Source 2needs review

The photoactivatable CRISPR-Cas9 system provides a therapeutic strategy for temporal control of reproductive functions in vivo.

and provides a therapeutic strategy for temporal control of reproductive functions in vivo
Claim 20therapeutic potentialsupports2020Source 2needs review

The photoactivatable CRISPR-Cas9 system provides a therapeutic strategy for temporal control of reproductive functions in vivo.

and provides a therapeutic strategy for temporal control of reproductive functions in vivo
Claim 21therapeutic potentialsupports2020Source 2needs review

The photoactivatable CRISPR-Cas9 system provides a therapeutic strategy for temporal control of reproductive functions in vivo.

and provides a therapeutic strategy for temporal control of reproductive functions in vivo
Claim 22therapeutic potentialsupports2020Source 2needs review

The photoactivatable CRISPR-Cas9 system provides a therapeutic strategy for temporal control of reproductive functions in vivo.

and provides a therapeutic strategy for temporal control of reproductive functions in vivo
Claim 23therapeutic potentialsupports2020Source 2needs review

The photoactivatable CRISPR-Cas9 system provides a therapeutic strategy for temporal control of reproductive functions in vivo.

and provides a therapeutic strategy for temporal control of reproductive functions in vivo

Approval Evidence

2 sources3 linked approval claimsfirst-pass slugs light-emitting-diode-illumination, light-emitting-diode-technology
The review addresses how the ubiquitous introduction of light-emitting diode technology may exacerbate, or in some cases reduce, the generalized ever-increasing light pollution.

Source:

illumination with a light-emitting diode

Source:

gene knockdown effectsupports

In mice, LIF knockdown using a photoactivatable CRISPR-Cas9 system with light-emitting diode illumination can spatiotemporally disrupt fertility.

Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility.

Source:

mitigation summarysupports

Reasonable use of lighting technology, including restricting illumination to essential areas and hours, directing lighting to avoid wasteful radiation, and selecting spectral emissions, is discussed as a way to reduce impacts on circadian clocks.

The article concludes by debating how these anthropogenic changes could be mitigated by more reasonable use of available technology - for example by restricting illumination to more essential areas and hours, directing lighting to avoid wasteful radiation and selecting spectral emissions, to reduce impact on circadian clocks.

Source:

technology impact summarymixed

The introduction of light-emitting diode technology may either exacerbate or in some cases reduce increasing light pollution.

The review addresses how the ubiquitous introduction of light-emitting diode technology may exacerbate, or in some cases reduce, the generalized ever-increasing light pollution.

Source:

Comparisons

Source-backed strengths

The available evidence supports in vivo use in mice in conjunction with photoactivatable CRISPR-Cas9. The associated study links LED illumination-enabled activation to spatiotemporal perturbation of fertility and to investigation of embryo implantation mechanisms.

Ranked Citations

  1. 1.
    StructuralSource 1Frontiers in Neuroscience2020Claim 15Claim 16

    Seeded from load plan for claim cl4. Extracted from this source document.

  2. 2.
    StructuralSource 2Proceedings of the National Academy of Sciences2020Claim 1Claim 2Claim 3

    Extracted from this source document.