Source 1primary paper2018Scholarly Commons (University of Pennsylvania)
Toolkit/light-induced hetero-dimerization system
light-induced hetero-dimerization system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Opto-RGS2 is an optogenetic multi-component switch that uses a light-induced heterodimerization system to recruit the RGS2 domain to the plasma membrane, where it interacts with its cognate G protein. It was developed to enable optical control of Gq-protein signaling and associated calcium oscillation dynamics.
Usefulness & Problems
Why this is useful
This system is useful for perturbing Gq-linked signaling with light by controlling membrane recruitment of an RGS2 effector domain. In an engineered cell-line context, it supported optical re-creation of calcium oscillation patterns that independently varied a single waveform component.
Source:
Using our engineered opto-RGS2 cell line, we revealed that RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
Source:
First, we created optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
Problem solved
The tool addresses the problem of controlling G-protein signaling with sufficient temporal precision to study how specific features of calcium oscillations encode information. It specifically enables light-dependent recruitment of RGS2 as a feedback regulator within a Gq-protein signaling circuit.
Source:
First, we created optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
Implementation involved engineering a light-induced heterodimerization system that recruits the RGS2 domain to the membrane. The available evidence also indicates use of an optogenetically engineered cell line, a mathematical model, and custom hardware, but does not specify construct architecture, expression system details, or chromophore requirements.
The supplied evidence does not identify the heterodimerization pair, illumination wavelength, kinetic performance, or quantitative dynamic range. Validation is described in an engineered cell line and a calcium-signaling application, with no independent replication or broader organismal testing provided here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Using a mathematical model, an optogenetically engineered cell line, and custom hardware, the authors optically re-created patterns of calcium oscillations that independently varied a single waveform component.
Using a mathematical model, an optogenetically-engineered cell line, and custom hardware, we optically re-created patterns of calcium oscillations that independently varied a single waveform component.
Using a mathematical model, an optogenetically engineered cell line, and custom hardware, the authors optically re-created patterns of calcium oscillations that independently varied a single waveform component.
Using a mathematical model, an optogenetically-engineered cell line, and custom hardware, we optically re-created patterns of calcium oscillations that independently varied a single waveform component.
Using a mathematical model, an optogenetically engineered cell line, and custom hardware, the authors optically re-created patterns of calcium oscillations that independently varied a single waveform component.
Using a mathematical model, an optogenetically-engineered cell line, and custom hardware, we optically re-created patterns of calcium oscillations that independently varied a single waveform component.
Using a mathematical model, an optogenetically engineered cell line, and custom hardware, the authors optically re-created patterns of calcium oscillations that independently varied a single waveform component.
Using a mathematical model, an optogenetically-engineered cell line, and custom hardware, we optically re-created patterns of calcium oscillations that independently varied a single waveform component.
Using a mathematical model, an optogenetically engineered cell line, and custom hardware, the authors optically re-created patterns of calcium oscillations that independently varied a single waveform component.
Using a mathematical model, an optogenetically-engineered cell line, and custom hardware, we optically re-created patterns of calcium oscillations that independently varied a single waveform component.
Using a mathematical model, an optogenetically engineered cell line, and custom hardware, the authors optically re-created patterns of calcium oscillations that independently varied a single waveform component.
Using a mathematical model, an optogenetically-engineered cell line, and custom hardware, we optically re-created patterns of calcium oscillations that independently varied a single waveform component.
Using a mathematical model, an optogenetically engineered cell line, and custom hardware, the authors optically re-created patterns of calcium oscillations that independently varied a single waveform component.
Using a mathematical model, an optogenetically-engineered cell line, and custom hardware, we optically re-created patterns of calcium oscillations that independently varied a single waveform component.
Using the engineered opto-RGS2 cell line, RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
Using our engineered opto-RGS2 cell line, we revealed that RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
Using the engineered opto-RGS2 cell line, RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
Using our engineered opto-RGS2 cell line, we revealed that RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
Using the engineered opto-RGS2 cell line, RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
Using our engineered opto-RGS2 cell line, we revealed that RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
Using the engineered opto-RGS2 cell line, RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
Using our engineered opto-RGS2 cell line, we revealed that RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
Using the engineered opto-RGS2 cell line, RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
Using our engineered opto-RGS2 cell line, we revealed that RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
Using the engineered opto-RGS2 cell line, RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
Using our engineered opto-RGS2 cell line, we revealed that RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
Using the engineered opto-RGS2 cell line, RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
Using our engineered opto-RGS2 cell line, we revealed that RGS2 reduced periodicity and stochasticity of G-protein coupled calcium oscillations and acted as a feedback regulator in this signaling circuit.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
The authors developed optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
First, we created optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
The authors developed optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
First, we created optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
The authors developed optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
First, we created optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
The authors developed optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
First, we created optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
The authors developed optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
First, we created optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
The authors developed optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
First, we created optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
The authors developed optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
First, we created optogenetic RGS2 (opto-RGS2) for studying calcium encoding.
Approval Evidence
1 source1 linked approval claimfirst-pass slug light-induced-hetero-dimerization-system
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
Source:
mechanismsupports
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
A light-induced hetero-dimerization system was engineered to recruit the RGS2 domain to the membrane where it interacted with its cognate G protein.
Source:
Comparisons
Source-backed strengths
The reported design provides mechanistic specificity through light-induced membrane recruitment of the RGS2 domain to its site of action at the membrane. In the engineered opto-RGS2 cell line, RGS2 reduced the periodicity and stochasticity of G-protein-coupled calcium oscillations and functioned as a feedback regulator.
Ranked Citations
- 1.