Source 1primary paper2022TSpace
Toolkit/light inducible dimer pairs
light inducible dimer pairs
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Light inducible dimer pairs are multicomponent optogenetic switches derived from cyanobacteriochrome photoswitchable proteins. They enable light-dependent heterodimerization and were developed to support orthogonal control of biological processes with red, green, and blue light.
Usefulness & Problems
Why this is useful
These dimer pairs are useful for multichromatic optogenetic control because they provide separable light inputs across red, green, and blue wavelengths. The underlying cyanobacteriochrome family is noted to respond across a broad spectral range from the UV to the near infra-red, supporting the rationale for wavelength-diverse control.
Source:
permitting orthogonal control of biological processes with red-, green-, and blue-light
Source:
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
Problem solved
This tool addresses the problem of achieving orthogonal optical control of biological processes using multiple colors of light. It specifically helps expand beyond single-channel optogenetic regulation by providing distinct light-inducible dimerization pairs derived from cyanobacteriochromes.
Source:
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
HeterodimerizationTechniques
Directed EvolutionTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The available evidence indicates that these tools are based on cyanobacteriochrome photoswitchable proteins and function as inducible dimer pairs controlled by light. However, the evidence provided does not specify construct architecture, required cofactors or chromophores, expression strategy, or delivery method.
The supplied evidence does not report quantitative performance metrics such as binding affinity, dynamic range, switching kinetics, reversibility, or background interaction. It also does not specify the exact protein pairs, chromophore requirements, host systems, or extent of validation in different biological contexts.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
Approval Evidence
1 source2 linked approval claimsfirst-pass slug light-inducible-dimer-pairs
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches, permitting orthogonal control of biological processes with red-, green-, and blue-light.
Source:
functional capabilitysupports
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
Source:
tool developmentsupports
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
Source:
Comparisons
Source-backed strengths
A key strength is the reported ability to permit orthogonal control with red-, green-, and blue-light. Another strength is that the tool was developed from cyanobacteriochrome photoswitchable proteins, a class with broad spectral responsiveness extending from UV to near infra-red.
Ranked Citations
- 1.