Source 1primary paper2020Science Advances
Toolkit/light-inducible nuclear translocation and dimerization system
light-inducible nuclear translocation and dimerization system
Also known as: LINTAD, LINTAD system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
LINTAD is a multi-component light-inducible nuclear translocation and dimerization system developed for gene regulation. In the cited study, it was used to control chimeric antigen receptor (CAR) T-cell activation, and pulsed light stimulation activated LINTAD-engineered CAR T cells to produce strong cytotoxicity against target cancer cells in vitro and in vivo.
Usefulness & Problems
Why this is useful
This system is useful for optical control of cellular localization and gene regulation in engineered immune cells. The cited work specifically supports its use for regulating CAR T-cell activation with pulsed light and for eliciting antitumor cytotoxicity in vitro and in vivo.
Source:
The results showed that pulsed light stimulations can activate LINTAD CAR T cells with strong cytotoxicity against target cancer cells, both in vitro and in vivo.
Source:
We first demonstrated light-controllable gene expression and functional modulation in human embryonic kidney 293T and Jurkat T cell lines.
Source:
Therefore, our LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
Problem solved
LINTAD addresses the problem of controlling CAR T-cell activation through a light-responsive gene regulation system. The available evidence indicates that it enables externally triggered activation of engineered CAR T cells using light.
Source:
The results showed that pulsed light stimulations can activate LINTAD CAR T cells with strong cytotoxicity against target cancer cells, both in vitro and in vivo.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
localizationInput: Light
Implementation Constraints
LINTAD is described as a multi-component system based on light-inducible nuclear translocation and dimerization for gene regulation. The supplied evidence supports implementation in engineered CAR T cells with pulsed light stimulation, but it does not provide construct architecture, cofactor requirements, delivery method, or expression details.
The provided evidence does not specify the molecular components, photoreceptor domains, wavelengths, kinetics, dynamic range, or reversibility of the system. Independent replication is not provided, and validation is only documented here in the context of CAR T-cell control from a single study.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2review2024Discovery Immunology
Ranked Claims
Pulsed light stimulation activated LINTAD CAR T cells and produced strong cytotoxicity against target cancer cells in vitro and in vivo.
The results showed that pulsed light stimulations can activate LINTAD CAR T cells with strong cytotoxicity against target cancer cells, both in vitro and in vivo.
Pulsed light stimulation activated LINTAD CAR T cells and produced strong cytotoxicity against target cancer cells in vitro and in vivo.
The results showed that pulsed light stimulations can activate LINTAD CAR T cells with strong cytotoxicity against target cancer cells, both in vitro and in vivo.
Pulsed light stimulation activated LINTAD CAR T cells and produced strong cytotoxicity against target cancer cells in vitro and in vivo.
The results showed that pulsed light stimulations can activate LINTAD CAR T cells with strong cytotoxicity against target cancer cells, both in vitro and in vivo.
Pulsed light stimulation activated LINTAD CAR T cells and produced strong cytotoxicity against target cancer cells in vitro and in vivo.
The results showed that pulsed light stimulations can activate LINTAD CAR T cells with strong cytotoxicity against target cancer cells, both in vitro and in vivo.
Pulsed light stimulation activated LINTAD CAR T cells and produced strong cytotoxicity against target cancer cells in vitro and in vivo.
The results showed that pulsed light stimulations can activate LINTAD CAR T cells with strong cytotoxicity against target cancer cells, both in vitro and in vivo.
Pulsed light stimulation activated LINTAD CAR T cells and produced strong cytotoxicity against target cancer cells in vitro and in vivo.
The results showed that pulsed light stimulations can activate LINTAD CAR T cells with strong cytotoxicity against target cancer cells, both in vitro and in vivo.
Pulsed light stimulation activated LINTAD CAR T cells and produced strong cytotoxicity against target cancer cells in vitro and in vivo.
The results showed that pulsed light stimulations can activate LINTAD CAR T cells with strong cytotoxicity against target cancer cells, both in vitro and in vivo.
The authors developed the LINTAD system for gene regulation to control CAR T activation.
Here, we developed a light-inducible nuclear translocation and dimerization (LINTAD) system for gene regulation to control CAR T activation.
The authors developed the LINTAD system for gene regulation to control CAR T activation.
Here, we developed a light-inducible nuclear translocation and dimerization (LINTAD) system for gene regulation to control CAR T activation.
The authors developed the LINTAD system for gene regulation to control CAR T activation.
Here, we developed a light-inducible nuclear translocation and dimerization (LINTAD) system for gene regulation to control CAR T activation.
The authors developed the LINTAD system for gene regulation to control CAR T activation.
Here, we developed a light-inducible nuclear translocation and dimerization (LINTAD) system for gene regulation to control CAR T activation.
The authors developed the LINTAD system for gene regulation to control CAR T activation.
Here, we developed a light-inducible nuclear translocation and dimerization (LINTAD) system for gene regulation to control CAR T activation.
The authors developed the LINTAD system for gene regulation to control CAR T activation.
Here, we developed a light-inducible nuclear translocation and dimerization (LINTAD) system for gene regulation to control CAR T activation.
The authors developed the LINTAD system for gene regulation to control CAR T activation.
Here, we developed a light-inducible nuclear translocation and dimerization (LINTAD) system for gene regulation to control CAR T activation.
The LINTAD system enabled light-controllable gene expression and functional modulation in HEK293T and Jurkat T cell lines.
We first demonstrated light-controllable gene expression and functional modulation in human embryonic kidney 293T and Jurkat T cell lines.
The LINTAD system enabled light-controllable gene expression and functional modulation in HEK293T and Jurkat T cell lines.
We first demonstrated light-controllable gene expression and functional modulation in human embryonic kidney 293T and Jurkat T cell lines.
The LINTAD system enabled light-controllable gene expression and functional modulation in HEK293T and Jurkat T cell lines.
We first demonstrated light-controllable gene expression and functional modulation in human embryonic kidney 293T and Jurkat T cell lines.
The LINTAD system enabled light-controllable gene expression and functional modulation in HEK293T and Jurkat T cell lines.
We first demonstrated light-controllable gene expression and functional modulation in human embryonic kidney 293T and Jurkat T cell lines.
The LINTAD system enabled light-controllable gene expression and functional modulation in HEK293T and Jurkat T cell lines.
We first demonstrated light-controllable gene expression and functional modulation in human embryonic kidney 293T and Jurkat T cell lines.
The LINTAD system enabled light-controllable gene expression and functional modulation in HEK293T and Jurkat T cell lines.
We first demonstrated light-controllable gene expression and functional modulation in human embryonic kidney 293T and Jurkat T cell lines.
The LINTAD system enabled light-controllable gene expression and functional modulation in HEK293T and Jurkat T cell lines.
We first demonstrated light-controllable gene expression and functional modulation in human embryonic kidney 293T and Jurkat T cell lines.
The LINTAD system was improved to achieve optimal efficiency in primary human T cells.
We then improved the LINTAD system to achieve optimal efficiency in primary human T cells.
The LINTAD system was improved to achieve optimal efficiency in primary human T cells.
We then improved the LINTAD system to achieve optimal efficiency in primary human T cells.
The LINTAD system was improved to achieve optimal efficiency in primary human T cells.
We then improved the LINTAD system to achieve optimal efficiency in primary human T cells.
The LINTAD system was improved to achieve optimal efficiency in primary human T cells.
We then improved the LINTAD system to achieve optimal efficiency in primary human T cells.
The LINTAD system was improved to achieve optimal efficiency in primary human T cells.
We then improved the LINTAD system to achieve optimal efficiency in primary human T cells.
The LINTAD system was improved to achieve optimal efficiency in primary human T cells.
We then improved the LINTAD system to achieve optimal efficiency in primary human T cells.
The LINTAD system was improved to achieve optimal efficiency in primary human T cells.
We then improved the LINTAD system to achieve optimal efficiency in primary human T cells.
The LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
Therefore, our LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
The LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
Therefore, our LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
The LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
Therefore, our LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
The LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
Therefore, our LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
The LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
Therefore, our LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
The LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
Therefore, our LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
The LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
Therefore, our LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
Approval Evidence
2 sources5 linked approval claimsfirst-pass slugs light-inducible-nuclear-translocation-and-dimerization-system, lintad
Explicitly supported tool/component names found in sources include LiCAR, optoCAR, LiTE system, PA-CXCR4, TamPA-Cre, and LINTAD/WW-LINTAD.
Source:
Here, we developed a light-inducible nuclear translocation and dimerization (LINTAD) system for gene regulation to control CAR T activation.
Source:
application performancesupports
Pulsed light stimulation activated LINTAD CAR T cells and produced strong cytotoxicity against target cancer cells in vitro and in vivo.
The results showed that pulsed light stimulations can activate LINTAD CAR T cells with strong cytotoxicity against target cancer cells, both in vitro and in vivo.
Source:
developmentsupports
The authors developed the LINTAD system for gene regulation to control CAR T activation.
Here, we developed a light-inducible nuclear translocation and dimerization (LINTAD) system for gene regulation to control CAR T activation.
Source:
functional capabilitysupports
The LINTAD system enabled light-controllable gene expression and functional modulation in HEK293T and Jurkat T cell lines.
We first demonstrated light-controllable gene expression and functional modulation in human embryonic kidney 293T and Jurkat T cell lines.
Source:
optimizationsupports
The LINTAD system was improved to achieve optimal efficiency in primary human T cells.
We then improved the LINTAD system to achieve optimal efficiency in primary human T cells.
Source:
utilitysupports
The LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
Therefore, our LINTAD system can serve as an efficient tool to noninvasively control gene activation and activate inducible CAR T cells for precision cancer immunotherapy.
Source:
Comparisons
Source-backed strengths
The cited study reports that pulsed light stimulation activated LINTAD CAR T cells and produced strong cytotoxicity against target cancer cells in vitro and in vivo. The system was explicitly developed as a light-inducible nuclear translocation and dimerization platform for gene regulation, supporting its intended function in optically controlled cell engineering.
Source:
We then improved the LINTAD system to achieve optimal efficiency in primary human T cells.
Ranked Citations
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Extracted from this source document.