Source 1primary paper2013Nature
Toolkit/light-inducible transcriptional effectors
light-inducible transcriptional effectors
Also known as: LITEs, LITE system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Light-inducible transcriptional effectors (LITEs) are an optogenetic two-hybrid system that combines a customizable TALE DNA-binding domain with Arabidopsis thaliana CRY2 and its interacting partner CIB1. The system enables light-triggered, reversible control of endogenous mammalian gene expression and targeted epigenetic chromatin modification.
Usefulness & Problems
Why this is useful
LITEs provide optical control over endogenous transcriptional and chromatin-regulatory processes in mammalian cells and tissues. The system is useful because it can be customized to many genomic loci, activated within minutes, reversed after stimulation, and does not require exogenous chemical cofactors.
Source:
We have applied this system in primary mouse neurons, as well as in the brain of freely behaving mice in vivo to mediate reversible modulation of mammalian endogenous gene expression as well as targeted epigenetic chromatin modifications.
Source:
Here we describe the development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana.
Problem solved
LITEs address the problem of achieving reversible, locus-specific control of endogenous mammalian gene expression and epigenetic state using light rather than diffusible chemicals. The reported platform also helps solve targeting and delivery constraints by being compatible with viral vectors and genetic targeting to specific cell populations.
Source:
We have applied this system in primary mouse neurons, as well as in the brain of freely behaving mice in vivo to mediate reversible modulation of mammalian endogenous gene expression as well as targeted epigenetic chromatin modifications.
Source:
Here we describe the development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-induced protein-protein interactionoptogenetic two-hybrid recruitmenttargeted epigenetic chromatin modificationtranscriptional regulationTechniques
No technique tags yet.
Target processes
transcriptionInput: Light
Implementation Constraints
LITEs are multi-component constructs built by integrating a TALE DNA-binding domain with the light-sensitive CRY2 protein and its binding partner CIB1 from Arabidopsis thaliana. The system can be packaged into viral vectors and genetically targeted to specific cell populations, and it does not require additional exogenous chemical cofactors.
The supplied evidence describes the platform at a high level but does not provide quantitative performance metrics such as dynamic range, off-target activity, illumination parameters, or durability across targets. Evidence is drawn from a single 2013 source, so independent replication is not established here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2review2024Discovery Immunology
Ranked Claims
The LITE system was applied in primary mouse neurons and in the brain of freely behaving mice in vivo to mediate reversible modulation of mammalian endogenous gene expression and targeted epigenetic chromatin modifications.
We have applied this system in primary mouse neurons, as well as in the brain of freely behaving mice in vivo to mediate reversible modulation of mammalian endogenous gene expression as well as targeted epigenetic chromatin modifications.
LITEs can be packaged into viral vectors and genetically targeted to probe specific cell populations.
LITEs can be packaged into viral vectors and genetically targeted to probe specific cell populations.
The LITE system establishes a novel mode of optogenetic control of endogenous cellular processes.
The LITE system establishes a novel mode of optogenetic control of endogenous cellular processes
LITEs do not require additional exogenous chemical cofactors, can be customized to target many endogenous genomic loci, and can be activated within minutes with reversibility.
LITEs do not require additional exogenous chemical cofactors, are easily customized to target many endogenous genomic loci, and can be activated within minutes with reversibility.
activation time within minutes
The paper describes development of LITEs, an optogenetic two-hybrid system integrating a TALE DNA-binding domain with cryptochrome 2 and CIB1.
Here we describe the development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana.
The paper describes development of LITEs, an optogenetic two-hybrid system integrating a TALE DNA-binding domain with cryptochrome 2 and CIB1.
Here we describe the development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana.
The paper describes development of LITEs, an optogenetic two-hybrid system integrating a TALE DNA-binding domain with cryptochrome 2 and CIB1.
Here we describe the development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana.
The paper describes development of LITEs, an optogenetic two-hybrid system integrating a TALE DNA-binding domain with cryptochrome 2 and CIB1.
Here we describe the development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana.
The paper describes development of LITEs, an optogenetic two-hybrid system integrating a TALE DNA-binding domain with cryptochrome 2 and CIB1.
Here we describe the development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana.
The paper describes development of LITEs, an optogenetic two-hybrid system integrating a TALE DNA-binding domain with cryptochrome 2 and CIB1.
Here we describe the development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana.
The paper describes development of LITEs, an optogenetic two-hybrid system integrating a TALE DNA-binding domain with cryptochrome 2 and CIB1.
Here we describe the development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana.
Approval Evidence
2 sources5 linked approval claimsfirst-pass slugs light-inducible-transcriptional-effectors, lite-system
Explicitly supported tool/component names found in sources include LiCAR, optoCAR, LiTE system, PA-CXCR4, TamPA-Cre, and LINTAD/WW-LINTAD.
Source:
Here we describe the development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana.
Source:
application resultsupports
The LITE system was applied in primary mouse neurons and in the brain of freely behaving mice in vivo to mediate reversible modulation of mammalian endogenous gene expression and targeted epigenetic chromatin modifications.
We have applied this system in primary mouse neurons, as well as in the brain of freely behaving mice in vivo to mediate reversible modulation of mammalian endogenous gene expression as well as targeted epigenetic chromatin modifications.
Source:
delivery and targeting propertysupports
LITEs can be packaged into viral vectors and genetically targeted to probe specific cell populations.
LITEs can be packaged into viral vectors and genetically targeted to probe specific cell populations.
Source:
novel capabilitysupports
The LITE system establishes a novel mode of optogenetic control of endogenous cellular processes.
The LITE system establishes a novel mode of optogenetic control of endogenous cellular processes
Source:
operational propertysupports
LITEs do not require additional exogenous chemical cofactors, can be customized to target many endogenous genomic loci, and can be activated within minutes with reversibility.
LITEs do not require additional exogenous chemical cofactors, are easily customized to target many endogenous genomic loci, and can be activated within minutes with reversibility.
Source:
tool developmentsupports
The paper describes development of LITEs, an optogenetic two-hybrid system integrating a TALE DNA-binding domain with cryptochrome 2 and CIB1.
Here we describe the development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana.
Source:
Comparisons
Source-backed strengths
The system was reported to function in primary mouse neurons and in the brains of freely behaving mice in vivo, supporting use in both cultured cells and intact mammalian tissue. Additional strengths include minute-scale activation, reversibility, lack of requirement for added chemical cofactors, customizable genomic targeting through the TALE DNA-binding module, and the ability to mediate targeted epigenetic chromatin modifications.
Ranked Citations
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