Source 1review2021International Journal of Molecular Sciences
Toolkit/light-regulated protein-protein interaction
light-regulated protein-protein interaction
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Light-regulated protein-protein interaction is a construct pattern in optogenetics that controls signaling protein function by coupling light input to regulated protein-protein interactions or to caging/uncaging events. It is presented as a general strategy for the spatiotemporal control of signal transduction pathways.
Usefulness & Problems
Why this is useful
This pattern is useful because it links light input to direct control over signaling protein function in a spatiotemporally regulated manner. The supplied evidence supports its use as a general optogenetic strategy for steering signal transduction, but does not provide system-specific performance data.
Problem solved
It addresses the problem of controlling signaling proteins with light through inducible protein-protein interactions or caging/uncaging-based regulation. The evidence specifically frames this as a solution for spatiotemporal control of signal transduction pathways.
Problem links
Need conditional control of signaling activity
DerivedThis construct pattern comprises optogenetic approaches that steer signaling protein function by coupling light input to regulated protein-protein interactions or to caging/uncaging events. It is a general strategy for light-dependent control of signal transduction.
Need precise spatiotemporal control with light input
DerivedThis construct pattern comprises optogenetic approaches that steer signaling protein function by coupling light input to regulated protein-protein interactions or to caging/uncaging events. It is a general strategy for light-dependent control of signal transduction.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
conformational uncagingconformational uncagingConformational Uncaginglight-regulated protein-protein interactionlight-regulated protein-protein interactionTechniques
No technique tags yet.
Target processes
signalingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: uncaging
The available evidence supports domain fusion as an engineering technique used in this construct pattern. However, the supplied text does not specify construct architectures, cofactors, expression systems, delivery methods, or organism-specific implementation details.
The evidence is limited to a high-level review statement and does not specify particular photoreceptors, wavelengths, kinetics, dynamic range, or validated biological contexts. Independent experimental replication, quantitative performance, and implementation constraints are not described in the supplied material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
Approval Evidence
1 source1 linked approval claimfirst-pass slug light-regulated-protein-protein-interaction
optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
Source:
mechanism summarysupports
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
Source:
Comparisons
Source-backed strengths
The cited review identifies two supported operational modes within this pattern: light-regulated protein-protein interactions and caging/uncaging events. Its principal strength in the supplied evidence is conceptual breadth as a generalizable optogenetic strategy for signaling control, rather than a single tool-specific benchmark.
Compared with caging/uncaging events
light-regulated protein-protein interaction and caging/uncaging events address a similar problem space because they share signaling.
Shared frame: shared target processes: signaling; shared mechanisms: conformational uncaging, conformational_uncaging; same primary input modality: light
Strengths here: looks easier to implement in practice.
Compared with engineered focal adhesion kinase two-input gate
light-regulated protein-protein interaction and engineered focal adhesion kinase two-input gate address a similar problem space because they share signaling.
Shared frame: shared target processes: signaling; shared mechanisms: conformational uncaging, conformational_uncaging; same primary input modality: light
Strengths here: looks easier to implement in practice.
Compared with NIR Rac1 biosensor
light-regulated protein-protein interaction and NIR Rac1 biosensor address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling; same primary input modality: light
Ranked Citations
- 1.