Source 1primary paper2021PLANT PHYSIOLOGY
Toolkit/light-sheet fluorescence microscopy
light-sheet fluorescence microscopy
Also known as: LSFM
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
recent applications and reasonable expectations from current light-sheet fluorescence microscopy (LSFM) and super-resolution microscopy (SRM) modalities
Usefulness & Problems
Why this is useful
LSFM is described as a current imaging modality used to image plant subcellular compartments, cells, tissues, and organs. The review frames it as part of integrative, scalable approaches for spatiotemporal resolution of dynamic plant processes.; multiscale imaging of plant subcellular compartments, cells, tissues, and organs; spatiotemporal documentation of plant growth and development
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LSFM is described as a current imaging modality used to image plant subcellular compartments, cells, tissues, and organs. The review frames it as part of integrative, scalable approaches for spatiotemporal resolution of dynamic plant processes.
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multiscale imaging of plant subcellular compartments, cells, tissues, and organs
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spatiotemporal documentation of plant growth and development
Problem solved
It helps document plant growth and development across multiple organizational scales. The modality is positioned as useful for resolving dynamic processes in space and time.; supports integrative and scalable imaging of dynamic plant processes across levels of organization
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It helps document plant growth and development across multiple organizational scales. The modality is positioned as useful for resolving dynamic processes in space and time.
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supports integrative and scalable imaging of dynamic plant processes across levels of organization
Problem links
supports integrative and scalable imaging of dynamic plant processes across levels of organization
LiteratureIt helps document plant growth and development across multiple organizational scales. The modality is positioned as useful for resolving dynamic processes in space and time.
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It helps document plant growth and development across multiple organizational scales. The modality is positioned as useful for resolving dynamic processes in space and time.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The abstract states that living or fixed sample preparation methods and labeling strategies are important for plant applications. Plant sample accommodation is itself presented as a practical constraint.; requires plant-compatible sample preparation; requires labeling strategies successfully applied in plants
Existing LSFM has shortcomings for accommodating plant samples and can be limited by spherical aberrations and temporal restrictions for fast 3D cellular dynamics.; ability to accommodate plant samples is limited; documentation potential is affected by spherical aberrations; temporal restrictions can prohibit dynamic recording of fast cellular processes in three dimensions
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Light-sheet fluorescence microscopy and super-resolution microscopy have been translated to imaging of plant subcellular compartments, cells, tissues, and organs.
LSFM and SRM are expected to be bridged in the near future to achieve broader multiscale plant imaging with a single platform.
Existing LSFM and SRM have shortcomings in plant imaging, including limited accommodation of plant samples, spherical aberrations, and temporal restrictions that hinder recording of fast cellular processes in three dimensions.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug light-sheet-fluorescence-microscopy
recent applications and reasonable expectations from current light-sheet fluorescence microscopy (LSFM) and super-resolution microscopy (SRM) modalities
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application scopesupports
Light-sheet fluorescence microscopy and super-resolution microscopy have been translated to imaging of plant subcellular compartments, cells, tissues, and organs.
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future directionsupports
LSFM and SRM are expected to be bridged in the near future to achieve broader multiscale plant imaging with a single platform.
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limitationsupports
Existing LSFM and SRM have shortcomings in plant imaging, including limited accommodation of plant samples, spherical aberrations, and temporal restrictions that hinder recording of fast cellular processes in three dimensions.
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Comparisons
Source-stated alternatives
The abstract directly contrasts LSFM with super-resolution microscopy and suggests future bridging of LSFM and SRM on a single platform rather than treating either as fully sufficient alone.
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The abstract directly contrasts LSFM with super-resolution microscopy and suggests future bridging of LSFM and SRM on a single platform rather than treating either as fully sufficient alone.
Source-backed strengths
presented as part of an integrative and scalable imaging approach; applied to plant subcellular compartments, cells, tissues, and organs
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presented as part of an integrative and scalable imaging approach
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applied to plant subcellular compartments, cells, tissues, and organs
Compared with microscopy
The abstract directly contrasts LSFM with super-resolution microscopy and suggests future bridging of LSFM and SRM on a single platform rather than treating either as fully sufficient alone.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as part of an integrative and scalable imaging approach; applied to plant subcellular compartments, cells, tissues, and organs.
Relative tradeoffs: ability to accommodate plant samples is limited; documentation potential is affected by spherical aberrations; temporal restrictions can prohibit dynamic recording of fast cellular processes in three dimensions.
Source:
The abstract directly contrasts LSFM with super-resolution microscopy and suggests future bridging of LSFM and SRM on a single platform rather than treating either as fully sufficient alone.
Compared with super-resolution microscopy
The abstract directly contrasts LSFM with super-resolution microscopy and suggests future bridging of LSFM and SRM on a single platform rather than treating either as fully sufficient alone.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as part of an integrative and scalable imaging approach; applied to plant subcellular compartments, cells, tissues, and organs.
Relative tradeoffs: ability to accommodate plant samples is limited; documentation potential is affected by spherical aberrations; temporal restrictions can prohibit dynamic recording of fast cellular processes in three dimensions.
Source:
The abstract directly contrasts LSFM with super-resolution microscopy and suggests future bridging of LSFM and SRM on a single platform rather than treating either as fully sufficient alone.
Ranked Citations
- 1.