Source 1primary paper2017Scientific Reports
Toolkit/light-sheet microscopy
light-sheet microscopy
Also known as: single plane illumination
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Light-sheet microscopy, also termed single plane illumination microscopy, is an in vivo fluorescence imaging method tailored to larval research and embryonic imaging. The supplied evidence indicates that it can capture the full course of embryonic development from egg to larva and has been coupled with optogenetic perturbation to study Wnt signaling during embryogenesis.
Usefulness & Problems
Why this is useful
This method is useful for real-time in vivo observation of biological processes in transparent developmental systems such as embryos and larval zebrafish. The evidence also places it within microscopy toolkits matched to fluorescent probes for monitoring cell identity, fate, and physiology in living larvae.
Source:
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Problem solved
Light-sheet microscopy helps solve the problem of imaging developmental and physiological processes continuously in living organisms across extended time courses. The cited use case further shows that it supports simultaneous optical perturbation and readout for studying signal transduction in vivo during embryogenesis.
Problem links
Need conditional control of signaling activity
DerivedLight-sheet microscopy, also termed single plane illumination microscopy, is an in vivo imaging method used to observe biological processes across development and in larval model organisms. The supplied evidence indicates that it enables observation of embryonic development from egg to larva and has been coupled with optogenetics to study Wnt signaling during embryogenesis.
Need conditional recombination or state switching
DerivedLight-sheet microscopy, also termed single plane illumination microscopy, is an in vivo imaging method used to observe biological processes across development and in larval model organisms. The supplied evidence indicates that it enables observation of embryonic development from egg to larva and has been coupled with optogenetics to study Wnt signaling during embryogenesis.
Need precise spatiotemporal control with light input
DerivedLight-sheet microscopy, also termed single plane illumination microscopy, is an in vivo imaging method used to observe biological processes across development and in larval model organisms. The supplied evidence indicates that it enables observation of embryonic development from egg to larva and has been coupled with optogenetics to study Wnt signaling during embryogenesis.
Need tighter control over protein production
DerivedLight-sheet microscopy, also termed single plane illumination microscopy, is an in vivo imaging method used to observe biological processes across development and in larval model organisms. The supplied evidence indicates that it enables observation of embryonic development from egg to larva and has been coupled with optogenetics to study Wnt signaling during embryogenesis.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
coupled optogenetic perturbationfluorescence imagingfluorescence imagingsingle-plane optical illuminationsingle-plane optical illuminationTranslation ControlTechniques
Functional AssayTarget processes
recombinationsignalingtranslationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The evidence identifies this method as a fluorescence-based in vivo microscopy approach and explicitly notes the synonym single plane illumination microscopy. Reported implementations include microscopes tailored to in vivo larval research and experimental coupling with optogenetics for embryonic Wnt signaling studies; no further construct, hardware, or sample-preparation details are provided in the supplied text.
The provided evidence does not report quantitative performance metrics such as spatial resolution, imaging depth, phototoxicity, or temporal resolution. Validation in the supplied material is limited mainly to embryogenesis and larval zebrafish contexts, with no independent comparative benchmarking described.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2017
Source 3review2022FEBS Letters
Ranked Claims
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
Temporal inactivation of β-catenin confirmed that Wnt signaling is required for Drosophila pattern formation and for maintenance later in development.
Temporal inactivation of β–catenin confirmed that Wnt signaling is required not only for Drosophila pattern formation, but also for maintenance later in development.
Temporal inactivation of β-catenin confirmed that Wnt signaling is required for Drosophila pattern formation and for maintenance later in development.
Temporal inactivation of β–catenin confirmed that Wnt signaling is required not only for Drosophila pattern formation, but also for maintenance later in development.
Temporal inactivation of β-catenin confirmed that Wnt signaling is required for Drosophila pattern formation and for maintenance later in development.
Temporal inactivation of β–catenin confirmed that Wnt signaling is required not only for Drosophila pattern formation, but also for maintenance later in development.
Temporal inactivation of β-catenin confirmed that Wnt signaling is required for Drosophila pattern formation and for maintenance later in development.
Temporal inactivation of β–catenin confirmed that Wnt signaling is required not only for Drosophila pattern formation, but also for maintenance later in development.
Temporal inactivation of β-catenin confirmed that Wnt signaling is required for Drosophila pattern formation and for maintenance later in development.
Temporal inactivation of β–catenin confirmed that Wnt signaling is required not only for Drosophila pattern formation, but also for maintenance later in development.
Temporal inactivation of β-catenin confirmed that Wnt signaling is required for Drosophila pattern formation and for maintenance later in development.
Temporal inactivation of β–catenin confirmed that Wnt signaling is required not only for Drosophila pattern formation, but also for maintenance later in development.
Temporal inactivation of β-catenin confirmed that Wnt signaling is required for Drosophila pattern formation and for maintenance later in development.
Temporal inactivation of β–catenin confirmed that Wnt signaling is required not only for Drosophila pattern formation, but also for maintenance later in development.
Temporal inactivation of β-catenin confirmed that Wnt signaling is required for Drosophila pattern formation and for maintenance later in development.
Temporal inactivation of β–catenin confirmed that Wnt signaling is required not only for Drosophila pattern formation, but also for maintenance later in development.
Temporal inactivation of β-catenin confirmed that Wnt signaling is required for Drosophila pattern formation and for maintenance later in development.
Temporal inactivation of β–catenin confirmed that Wnt signaling is required not only for Drosophila pattern formation, but also for maintenance later in development.
Temporal inactivation of β-catenin confirmed that Wnt signaling is required for Drosophila pattern formation and for maintenance later in development.
Temporal inactivation of β–catenin confirmed that Wnt signaling is required not only for Drosophila pattern formation, but also for maintenance later in development.
Blue light illumination causes oligomerization of the CRY2-mCherry-Drosophila β-catenin fusion protein and inhibits downstream Wnt signaling in vitro and in vivo.
Blue light illumination caused oligomerization of the fusion protein and inhibited downstream Wnt signaling in vitro and in vivo .
Blue light illumination causes oligomerization of the CRY2-mCherry-Drosophila β-catenin fusion protein and inhibits downstream Wnt signaling in vitro and in vivo.
Blue light illumination caused oligomerization of the fusion protein and inhibited downstream Wnt signaling in vitro and in vivo .
Blue light illumination causes oligomerization of the CRY2-mCherry-Drosophila β-catenin fusion protein and inhibits downstream Wnt signaling in vitro and in vivo.
Blue light illumination caused oligomerization of the fusion protein and inhibited downstream Wnt signaling in vitro and in vivo .
Blue light illumination causes oligomerization of the CRY2-mCherry-Drosophila β-catenin fusion protein and inhibits downstream Wnt signaling in vitro and in vivo.
Blue light illumination caused oligomerization of the fusion protein and inhibited downstream Wnt signaling in vitro and in vivo .
Blue light illumination causes oligomerization of the CRY2-mCherry-Drosophila β-catenin fusion protein and inhibits downstream Wnt signaling in vitro and in vivo.
Blue light illumination caused oligomerization of the fusion protein and inhibited downstream Wnt signaling in vitro and in vivo .
Blue light illumination causes oligomerization of the CRY2-mCherry-Drosophila β-catenin fusion protein and inhibits downstream Wnt signaling in vitro and in vivo.
Blue light illumination caused oligomerization of the fusion protein and inhibited downstream Wnt signaling in vitro and in vivo .
Blue light illumination causes oligomerization of the CRY2-mCherry-Drosophila β-catenin fusion protein and inhibits downstream Wnt signaling in vitro and in vivo.
Blue light illumination caused oligomerization of the fusion protein and inhibited downstream Wnt signaling in vitro and in vivo .
Blue light illumination causes oligomerization of the CRY2-mCherry-Drosophila β-catenin fusion protein and inhibits downstream Wnt signaling in vitro and in vivo.
Blue light illumination caused oligomerization of the fusion protein and inhibited downstream Wnt signaling in vitro and in vivo .
Blue light illumination causes oligomerization of the CRY2-mCherry-Drosophila β-catenin fusion protein and inhibits downstream Wnt signaling in vitro and in vivo.
Blue light illumination caused oligomerization of the fusion protein and inhibited downstream Wnt signaling in vitro and in vivo .
Blue light illumination causes oligomerization of the CRY2-mCherry-Drosophila β-catenin fusion protein and inhibits downstream Wnt signaling in vitro and in vivo.
Blue light illumination caused oligomerization of the fusion protein and inhibited downstream Wnt signaling in vitro and in vivo .
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Approval Evidence
3 sources4 linked approval claimsfirst-pass slug light-sheet-microscopy
including confocal and light sheet (single plane illumination) microscopes tailored to in vivo larval research
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Light-sheet microscopy allows observation of the full course of embryonic development from egg to larva.
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Coupling optogenetics and light-sheet microscopy, a method to study Wnt signaling during embryogenesis
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review scope summarysupports
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
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translational positioningsupports
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
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method applicationsupports
The paper presents a method that couples optogenetics and light-sheet microscopy to study Wnt signaling during embryogenesis.
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method capabilitysupports
Coupling optogenetics and light-sheet microscopy allows precise temporal regulation studies of signaling pathways and cellular processes in vivo.
Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo .
Source:
Comparisons
Source-backed strengths
The supplied evidence states that light-sheet microscopy allows observation of the full course of embryonic development from egg to larva. It is also specifically described as being coupled with optogenetics to study Wnt signaling during embryogenesis, supporting its utility for dynamic in vivo functional assays.
Compared with confocal microscopy
light-sheet microscopy and confocal microscopy address a similar problem space because they share recombination, translation.
Shared frame: same top-level item type; shared target processes: recombination, translation; shared mechanisms: translation_control; same primary input modality: light
Strengths here: appears more independently replicated; looks easier to implement in practice.
Compared with optogenetic circuits
light-sheet microscopy and optogenetic circuits address a similar problem space because they share recombination, translation.
Shared frame: shared target processes: recombination, translation; shared mechanisms: translation_control; same primary input modality: light
Strengths here: appears more independently replicated; looks easier to implement in practice.
Compared with photobiomodulation therapy
light-sheet microscopy and photobiomodulation therapy address a similar problem space because they share signaling, translation.
Shared frame: shared target processes: signaling, translation; shared mechanisms: translation_control; same primary input modality: light
Strengths here: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
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