Source 1primary paper2023Nature Communications
Toolkit/light-switchable transcription factors
light-switchable transcription factors
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Light-switchable transcription factors are multi-component optogenetic regulators reported in a 2023 Nature Communications study. The available evidence indicates that they were obtained by direct screening in mammalian cells and are designed to enable light-dependent control of transcription factor activity.
Usefulness & Problems
Why this is useful
These tools are useful for imposing optical control over transcription in mammalian-cell contexts. The source title also links them to mammalian-cell screening workflows, but the available evidence does not provide performance details or specific application examples.
Problem solved
They address the problem of identifying transcription factors whose activity can be switched by light directly in mammalian cells. This is relevant to controlling transcription with an external light input in systems where mammalian-cell-compatible screening is required.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Target processes
recombinationselectiontranscriptionInput: Light
Implementation Constraints
The available evidence supports that the tool is multi-component and was screened in mammalian cells. No further implementation details are provided regarding construct architecture, cofactors, expression systems, delivery methods, or illumination parameters.
The supplied evidence is limited to the article title and does not specify the molecular components, photoreceptor domains, wavelengths, dynamic range, kinetics, or target genes. It also does not document validation across organisms, cell types, or downstream functions such as recombination or selection.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The paper reports light-switchable transcription factors obtained by direct screening in mammalian cells.
Light-switchable transcription factors obtained by direct screening in mammalian cells
The paper reports light-switchable transcription factors obtained by direct screening in mammalian cells.
Light-switchable transcription factors obtained by direct screening in mammalian cells
The paper reports light-switchable transcription factors obtained by direct screening in mammalian cells.
Light-switchable transcription factors obtained by direct screening in mammalian cells
The paper reports light-switchable transcription factors obtained by direct screening in mammalian cells.
Light-switchable transcription factors obtained by direct screening in mammalian cells
The paper reports light-switchable transcription factors obtained by direct screening in mammalian cells.
Light-switchable transcription factors obtained by direct screening in mammalian cells
The paper reports light-switchable transcription factors obtained by direct screening in mammalian cells.
Light-switchable transcription factors obtained by direct screening in mammalian cells
The paper reports light-switchable transcription factors obtained by direct screening in mammalian cells.
Light-switchable transcription factors obtained by direct screening in mammalian cells
Approval Evidence
1 source1 linked approval claimfirst-pass slug light-switchable-transcription-factors
Light-switchable transcription factors obtained by direct screening in mammalian cells
Source:
reported obtainment methodsupports
The paper reports light-switchable transcription factors obtained by direct screening in mammalian cells.
Light-switchable transcription factors obtained by direct screening in mammalian cells
Source:
Comparisons
Source-backed strengths
A key reported strength is that the light-switchable transcription factors were obtained by direct screening in mammalian cells, which supports compatibility with that cellular context. Beyond this obtainment method and the light-dependent transcriptional switching concept, the provided evidence does not report quantitative strengths.
Ranked Citations
- 1.