Source 1primary paper2023International Journal of Molecular Sciences
Toolkit/LightOn system
LightOn system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The LightOn system is an optogenetic gene-expression switch in which blue light induces activity of the GAVPO regulator to control expression of a gene of interest. In the cited 2023 work, all LightOn components were assembled into the OPTO-BLUE lentiviral platform for light-controlled reporter expression.
Usefulness & Problems
Why this is useful
This system is useful for modulating transgene expression with blue light rather than constitutive or chemically induced control. The cited study states that OPTO-BLUE should provide a molecular tool to modulate expression of any protein by blue light and demonstrated light-controlled reporter output dependent on illumination timing and intensity.
Source:
Likewise, this system should provide an important molecular tool to modulate gene expression of any protein by blue light.
Source:
Altogether, these results prove that the optimized OPTO-BLUE system allows the light-controlled expression of a reporter protein according to a specific time and light intensity.
Problem solved
It addresses the need for externally controllable, light-responsive regulation of gene expression. The 2023 implementation specifically solves the assembly problem by placing all components of the LightOn system into a single lentiviral plasmid.
Source:
Likewise, this system should provide an important molecular tool to modulate gene expression of any protein by blue light.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
dna binding-dependent transcriptional activationHeterodimerizationlight-induced dimerizationTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The cited implementation uses a lentiviral platform, with all LightOn components assembled into a single plasmid in the OPTO-BLUE design. Blue light is the inducing input, and the demonstrated output was reporter protein expression controlled by illumination timing and intensity.
The supplied evidence is limited to a 2023 report describing reporter regulation in the OPTO-BLUE lentiviral context. The provided material does not include quantitative dynamic range, leakiness, cell-type breadth, in vivo validation, or independent replication.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
OPTO-BLUE should provide a molecular tool to modulate expression of any protein by blue light.
Likewise, this system should provide an important molecular tool to modulate gene expression of any protein by blue light.
OPTO-BLUE should provide a molecular tool to modulate expression of any protein by blue light.
Likewise, this system should provide an important molecular tool to modulate gene expression of any protein by blue light.
OPTO-BLUE should provide a molecular tool to modulate expression of any protein by blue light.
Likewise, this system should provide an important molecular tool to modulate gene expression of any protein by blue light.
OPTO-BLUE should provide a molecular tool to modulate expression of any protein by blue light.
Likewise, this system should provide an important molecular tool to modulate gene expression of any protein by blue light.
OPTO-BLUE should provide a molecular tool to modulate expression of any protein by blue light.
Likewise, this system should provide an important molecular tool to modulate gene expression of any protein by blue light.
OPTO-BLUE should provide a molecular tool to modulate expression of any protein by blue light.
Likewise, this system should provide an important molecular tool to modulate gene expression of any protein by blue light.
OPTO-BLUE should provide a molecular tool to modulate expression of any protein by blue light.
Likewise, this system should provide an important molecular tool to modulate gene expression of any protein by blue light.
OPTO-BLUE assembles all components of the LightOn system into a single lentiviral plasmid.
Here, we continue the optimization and assemble all components of the LightOn system into a single lentiviral plasmid, the OPTO-BLUE system.
OPTO-BLUE assembles all components of the LightOn system into a single lentiviral plasmid.
Here, we continue the optimization and assemble all components of the LightOn system into a single lentiviral plasmid, the OPTO-BLUE system.
OPTO-BLUE assembles all components of the LightOn system into a single lentiviral plasmid.
Here, we continue the optimization and assemble all components of the LightOn system into a single lentiviral plasmid, the OPTO-BLUE system.
OPTO-BLUE assembles all components of the LightOn system into a single lentiviral plasmid.
Here, we continue the optimization and assemble all components of the LightOn system into a single lentiviral plasmid, the OPTO-BLUE system.
OPTO-BLUE assembles all components of the LightOn system into a single lentiviral plasmid.
Here, we continue the optimization and assemble all components of the LightOn system into a single lentiviral plasmid, the OPTO-BLUE system.
OPTO-BLUE assembles all components of the LightOn system into a single lentiviral plasmid.
Here, we continue the optimization and assemble all components of the LightOn system into a single lentiviral plasmid, the OPTO-BLUE system.
OPTO-BLUE assembles all components of the LightOn system into a single lentiviral plasmid.
Here, we continue the optimization and assemble all components of the LightOn system into a single lentiviral plasmid, the OPTO-BLUE system.
The optimized OPTO-BLUE system allows light-controlled expression of a reporter protein according to specific time and light intensity.
Altogether, these results prove that the optimized OPTO-BLUE system allows the light-controlled expression of a reporter protein according to a specific time and light intensity.
The optimized OPTO-BLUE system allows light-controlled expression of a reporter protein according to specific time and light intensity.
Altogether, these results prove that the optimized OPTO-BLUE system allows the light-controlled expression of a reporter protein according to a specific time and light intensity.
The optimized OPTO-BLUE system allows light-controlled expression of a reporter protein according to specific time and light intensity.
Altogether, these results prove that the optimized OPTO-BLUE system allows the light-controlled expression of a reporter protein according to a specific time and light intensity.
The optimized OPTO-BLUE system allows light-controlled expression of a reporter protein according to specific time and light intensity.
Altogether, these results prove that the optimized OPTO-BLUE system allows the light-controlled expression of a reporter protein according to a specific time and light intensity.
The optimized OPTO-BLUE system allows light-controlled expression of a reporter protein according to specific time and light intensity.
Altogether, these results prove that the optimized OPTO-BLUE system allows the light-controlled expression of a reporter protein according to a specific time and light intensity.
The optimized OPTO-BLUE system allows light-controlled expression of a reporter protein according to specific time and light intensity.
Altogether, these results prove that the optimized OPTO-BLUE system allows the light-controlled expression of a reporter protein according to a specific time and light intensity.
The optimized OPTO-BLUE system allows light-controlled expression of a reporter protein according to specific time and light intensity.
Altogether, these results prove that the optimized OPTO-BLUE system allows the light-controlled expression of a reporter protein according to a specific time and light intensity.
GAVPO dimerizes and binds the UASG sequence in response to blue light, triggering downstream transgene expression.
This system is based on a photosensitive protein (GAVPO), which dimerizes and binds to the UASG sequence in response to blue light, triggering the expression of a downstream transgene.
GAVPO dimerizes and binds the UASG sequence in response to blue light, triggering downstream transgene expression.
This system is based on a photosensitive protein (GAVPO), which dimerizes and binds to the UASG sequence in response to blue light, triggering the expression of a downstream transgene.
GAVPO dimerizes and binds the UASG sequence in response to blue light, triggering downstream transgene expression.
This system is based on a photosensitive protein (GAVPO), which dimerizes and binds to the UASG sequence in response to blue light, triggering the expression of a downstream transgene.
GAVPO dimerizes and binds the UASG sequence in response to blue light, triggering downstream transgene expression.
This system is based on a photosensitive protein (GAVPO), which dimerizes and binds to the UASG sequence in response to blue light, triggering the expression of a downstream transgene.
GAVPO dimerizes and binds the UASG sequence in response to blue light, triggering downstream transgene expression.
This system is based on a photosensitive protein (GAVPO), which dimerizes and binds to the UASG sequence in response to blue light, triggering the expression of a downstream transgene.
GAVPO dimerizes and binds the UASG sequence in response to blue light, triggering downstream transgene expression.
This system is based on a photosensitive protein (GAVPO), which dimerizes and binds to the UASG sequence in response to blue light, triggering the expression of a downstream transgene.
GAVPO dimerizes and binds the UASG sequence in response to blue light, triggering downstream transgene expression.
This system is based on a photosensitive protein (GAVPO), which dimerizes and binds to the UASG sequence in response to blue light, triggering the expression of a downstream transgene.
The LightOn system regulates expression of a gene of interest using blue light as an inducer.
The LightOn system is an optogenetic tool that regulates the expression of a gene of interest using blue light as an inducer.
The LightOn system regulates expression of a gene of interest using blue light as an inducer.
The LightOn system is an optogenetic tool that regulates the expression of a gene of interest using blue light as an inducer.
The LightOn system regulates expression of a gene of interest using blue light as an inducer.
The LightOn system is an optogenetic tool that regulates the expression of a gene of interest using blue light as an inducer.
The LightOn system regulates expression of a gene of interest using blue light as an inducer.
The LightOn system is an optogenetic tool that regulates the expression of a gene of interest using blue light as an inducer.
The LightOn system regulates expression of a gene of interest using blue light as an inducer.
The LightOn system is an optogenetic tool that regulates the expression of a gene of interest using blue light as an inducer.
The LightOn system regulates expression of a gene of interest using blue light as an inducer.
The LightOn system is an optogenetic tool that regulates the expression of a gene of interest using blue light as an inducer.
The LightOn system regulates expression of a gene of interest using blue light as an inducer.
The LightOn system is an optogenetic tool that regulates the expression of a gene of interest using blue light as an inducer.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug lighton-system
The LightOn system is an optogenetic tool that regulates the expression of a gene of interest using blue light as an inducer.
Source:
engineeringsupports
OPTO-BLUE assembles all components of the LightOn system into a single lentiviral plasmid.
Here, we continue the optimization and assemble all components of the LightOn system into a single lentiviral plasmid, the OPTO-BLUE system.
Source:
mechanismsupports
GAVPO dimerizes and binds the UASG sequence in response to blue light, triggering downstream transgene expression.
This system is based on a photosensitive protein (GAVPO), which dimerizes and binds to the UASG sequence in response to blue light, triggering the expression of a downstream transgene.
Source:
mechanismsupports
The LightOn system regulates expression of a gene of interest using blue light as an inducer.
The LightOn system is an optogenetic tool that regulates the expression of a gene of interest using blue light as an inducer.
Source:
Comparisons
Source-backed strengths
The reported OPTO-BLUE implementation integrates the full LightOn system into one lentiviral construct, which can simplify delivery relative to multi-vector configurations. The optimized system supported blue-light-controlled reporter expression that varied according to specific illumination time and light intensity.
Source:
Here, we continue the optimization and assemble all components of the LightOn system into a single lentiviral plasmid, the OPTO-BLUE system.
Ranked Citations
- 1.