Source 1primary paper2024Nucleic Acids Research
Toolkit/LightOnC.glu
LightOnC.glu
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
LightOnC.glu is a programmable light-responsive genetic circuit for Corynebacterium glutamicum that regulates gene expression in response to light signaling. It uses light-controlled RNA-binding proteins to build light-controlled transcription factors for dynamic transcriptional control.
Usefulness & Problems
Why this is useful
This tool provides optogenetic control of transcription in Corynebacterium glutamicum, enabling dynamic and programmable regulation without relying solely on conventional chemical induction. The reported platform also enabled engineered synthesis of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in this host.
Source:
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
Source:
LightOnC.glu is a gene expression regulation system in Corynebacterium glutamicum that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors.
Problem solved
It addresses the lack of a programmable light-responsive gene regulation platform in Corynebacterium glutamicum. The system supports dynamic control of engineered metabolic or regulatory networks based on light signaling.
Source:
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
Published Workflows
Objective: Develop dynamic light-controlled gene regulation tools in Corynebacterium glutamicum and apply them to rebalance metabolic flow for oligosaccharide production.
Why it works: The abstract frames the workflow around using light-responsive regulation to dynamically balance cell growth and product synthesis, then coupling that control to engineered synthesis pathways and a light-controlled bioreactor for production.
light-controlled RNA-binding-protein-mediated transcription controllight-controlled CRISPR/Cpf1-mediated gene interferencedynamic regulation based on light signalingoptogenetic controlgenetic circuit engineeringmetabolic engineeringbioreactor implementation
Stages
- 1.Establish light-controlled gene expression system(functional_characterization)
This stage establishes the core optogenetic expression-control capability needed before applying dynamic regulation to metabolic pathways.
Selection: Construction of light-controlled transcription factors using light-controlled RNA-binding proteins in C. glutamicum.
- 2.Develop light-controlled gene interference system(functional_characterization)
This stage adds a second regulatory mode, gene interference, to expand dynamic control over metabolic flow.
Selection: Development of a high-performance light-controlled gene interference system using CRISPR/Cpf1 tools.
- 3.Design synthesis network for target oligosaccharide production(library_design)
This stage connects the optogenetic control systems to a production objective by engineering the synthesis network for desired oligosaccharides.
Selection: Metabolic flow in the synthesis network was designed to enable CHOS and CSA production in C. glutamicum.
- 4.Bioreactor production validation(confirmatory_validation)
This stage confirms that the light-responsive system functions under production conditions and yields high CHOS titer.
Selection: Construction of a light-controlled bioreactor and measurement of CHOS production titer.
Objective: Develop dynamic, broadly applicable, minimally toxic light-responsive gene regulation in Corynebacterium glutamicum and use it to rebalance metabolic flow between growth and product synthesis.
Why it works: The workflow couples light-responsive control of gene expression and gene interference to dynamically redirect metabolic flow between growth and production states in C. glutamicum.
light-controlled RNA-binding-protein-mediated transcription controllight-controlled CRISPR/Cpf1-mediated gene interferenceoptogenetic controlgenetic circuit engineeringmetabolic engineeringbioreactor implementation
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
Computational DesignTarget processes
signalingtranscriptionInput: Light
Implementation Constraints
The system is implemented in Corynebacterium glutamicum and is described as a multi-component switch based on light-controlled RNA-binding proteins assembled into transcription factors. The supplied evidence does not specify the exact protein components, light wavelength, cofactors, promoter architecture, or delivery format.
The supplied evidence does not report quantitative performance metrics, spectral properties, response kinetics, leakiness, or reversibility. Validation is currently documented from a single 2024 study in Corynebacterium glutamicum, with no independent replication provided here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
The metabolic flow in the synthesis network was designed to enable the production of chitin oligosaccharides (CHOSs) and chondroitin sulphate oligosaccharides A (CSA) for the first time in C. glutamicum.
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
The metabolic flow in the synthesis network was designed to enable the production of chitin oligosaccharides (CHOSs) and chondroitin sulphate oligosaccharides A (CSA) for the first time in C. glutamicum.
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
The metabolic flow in the synthesis network was designed to enable the production of chitin oligosaccharides (CHOSs) and chondroitin sulphate oligosaccharides A (CSA) for the first time in C. glutamicum.
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
The metabolic flow in the synthesis network was designed to enable the production of chitin oligosaccharides (CHOSs) and chondroitin sulphate oligosaccharides A (CSA) for the first time in C. glutamicum.
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
The metabolic flow in the synthesis network was designed to enable the production of chitin oligosaccharides (CHOSs) and chondroitin sulphate oligosaccharides A (CSA) for the first time in C. glutamicum.
The study introduces a light-controlled gene expression system using light-controlled RNA-binding proteins in Corynebacterium glutamicum, described as a first for this organism.
This study introduces an advanced light-controlled gene expression system using light-controlled RNA-binding proteins (RBP), a first for Corynebacterium glutamicum.
The study introduces a light-controlled gene expression system using light-controlled RNA-binding proteins in Corynebacterium glutamicum, described as a first for this organism.
This study introduces an advanced light-controlled gene expression system using light-controlled RNA-binding proteins (RBP), a first for Corynebacterium glutamicum.
The study introduces a light-controlled gene expression system using light-controlled RNA-binding proteins in Corynebacterium glutamicum, described as a first for this organism.
This study introduces an advanced light-controlled gene expression system using light-controlled RNA-binding proteins (RBP), a first for Corynebacterium glutamicum.
The study introduces a light-controlled gene expression system using light-controlled RNA-binding proteins in Corynebacterium glutamicum, described as a first for this organism.
This study introduces an advanced light-controlled gene expression system using light-controlled RNA-binding proteins (RBP), a first for Corynebacterium glutamicum.
The study introduces a light-controlled gene expression system using light-controlled RNA-binding proteins in Corynebacterium glutamicum, described as a first for this organism.
This study introduces an advanced light-controlled gene expression system using light-controlled RNA-binding proteins (RBP), a first for Corynebacterium glutamicum.
A light-controlled bioreactor achieved a CHOSs production concentration of 6.2 g/L, described as the highest recorded titer for CHOSs biosynthesis to date.
Additionally, a light-controlled bioreactor was constructed, achieving a CHOSs production concentration of 6.2 g/L, the highest titer recorded for CHOSs biosynthesis to date.
CHOSs production concentration 6.2 g/Lrecord status highest titer recorded for CHOSs biosynthesis to date
A light-controlled bioreactor achieved a CHOSs production concentration of 6.2 g/L, described as the highest recorded titer for CHOSs biosynthesis to date.
Additionally, a light-controlled bioreactor was constructed, achieving a CHOSs production concentration of 6.2 g/L, the highest titer recorded for CHOSs biosynthesis to date.
CHOSs production concentration 6.2 g/Lrecord status highest titer recorded for CHOSs biosynthesis to date
A light-controlled bioreactor achieved a CHOSs production concentration of 6.2 g/L, described as the highest recorded titer for CHOSs biosynthesis to date.
Additionally, a light-controlled bioreactor was constructed, achieving a CHOSs production concentration of 6.2 g/L, the highest titer recorded for CHOSs biosynthesis to date.
CHOSs production concentration 6.2 g/Lrecord status highest titer recorded for CHOSs biosynthesis to date
A light-controlled bioreactor achieved a CHOSs production concentration of 6.2 g/L, described as the highest recorded titer for CHOSs biosynthesis to date.
Additionally, a light-controlled bioreactor was constructed, achieving a CHOSs production concentration of 6.2 g/L, the highest titer recorded for CHOSs biosynthesis to date.
CHOSs production concentration 6.2 g/Lrecord status highest titer recorded for CHOSs biosynthesis to date
A light-controlled bioreactor achieved a CHOSs production concentration of 6.2 g/L, described as the highest recorded titer for CHOSs biosynthesis to date.
Additionally, a light-controlled bioreactor was constructed, achieving a CHOSs production concentration of 6.2 g/L, the highest titer recorded for CHOSs biosynthesis to date.
CHOSs production concentration 6.2 g/Lrecord status highest titer recorded for CHOSs biosynthesis to date
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum for dynamic regulation based on light signaling.
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum for dynamic regulation based on light signaling.
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum for dynamic regulation based on light signaling.
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum for dynamic regulation based on light signaling.
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum for dynamic regulation based on light signaling.
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum for dynamic regulation based on light signaling.
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum for dynamic regulation based on light signaling.
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum for dynamic regulation based on light signaling.
The authors established LightOnC.glu as a gene expression regulation system that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors in Corynebacterium glutamicum.
We established a gene expression regulation system, 'LightOnC.glu', utilizing the light-controlled RBP to construct light-controlled transcription factors in C. glutamicum.
The authors established LightOnC.glu as a gene expression regulation system that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors in Corynebacterium glutamicum.
We established a gene expression regulation system, 'LightOnC.glu', utilizing the light-controlled RBP to construct light-controlled transcription factors in C. glutamicum.
The authors established LightOnC.glu as a gene expression regulation system that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors in Corynebacterium glutamicum.
We established a gene expression regulation system, 'LightOnC.glu', utilizing the light-controlled RBP to construct light-controlled transcription factors in C. glutamicum.
The authors established LightOnC.glu as a gene expression regulation system that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors in Corynebacterium glutamicum.
We established a gene expression regulation system, 'LightOnC.glu', utilizing the light-controlled RBP to construct light-controlled transcription factors in C. glutamicum.
The authors established LightOnC.glu as a gene expression regulation system that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors in Corynebacterium glutamicum.
We established a gene expression regulation system, 'LightOnC.glu', utilizing the light-controlled RBP to construct light-controlled transcription factors in C. glutamicum.
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum.
Herein, we have established a programmable light-responsive genetic circuit in C. glutamicum, advancing the theory of dynamic regulation based on light signaling.
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum.
Herein, we have established a programmable light-responsive genetic circuit in C. glutamicum, advancing the theory of dynamic regulation based on light signaling.
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum.
Herein, we have established a programmable light-responsive genetic circuit in C. glutamicum, advancing the theory of dynamic regulation based on light signaling.
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum.
Herein, we have established a programmable light-responsive genetic circuit in C. glutamicum, advancing the theory of dynamic regulation based on light signaling.
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum.
Herein, we have established a programmable light-responsive genetic circuit in C. glutamicum, advancing the theory of dynamic regulation based on light signaling.
LightOnC.glu is a gene expression regulation system in Corynebacterium glutamicum that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors.
LightOnC.glu is a gene expression regulation system in Corynebacterium glutamicum that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors.
LightOnC.glu is a gene expression regulation system in Corynebacterium glutamicum that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors.
LightOnC.glu is a gene expression regulation system in Corynebacterium glutamicum that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors.
LightOnC.glu is a gene expression regulation system in Corynebacterium glutamicum that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors.
LightOnC.glu is a gene expression regulation system in Corynebacterium glutamicum that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors.
LightOnC.glu is a gene expression regulation system in Corynebacterium glutamicum that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors.
LightOnC.glu is a gene expression regulation system in Corynebacterium glutamicum that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors.
Approval Evidence
1 source7 linked approval claimsfirst-pass slug lightonc-glu
We established a gene expression regulation system, 'LightOnC.glu', utilizing the light-controlled RBP to construct light-controlled transcription factors in C. glutamicum.
Source:
application enablementsupports
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
Source:
application enablementsupports
The engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
The metabolic flow in the synthesis network was designed to enable the production of chitin oligosaccharides (CHOSs) and chondroitin sulphate oligosaccharides A (CSA) for the first time in C. glutamicum.
Source:
novel tool introductionsupports
The study introduces a light-controlled gene expression system using light-controlled RNA-binding proteins in Corynebacterium glutamicum, described as a first for this organism.
This study introduces an advanced light-controlled gene expression system using light-controlled RNA-binding proteins (RBP), a first for Corynebacterium glutamicum.
Source:
platform capabilitysupports
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum for dynamic regulation based on light signaling.
Source:
tool establishmentsupports
The authors established LightOnC.glu as a gene expression regulation system that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors in Corynebacterium glutamicum.
We established a gene expression regulation system, 'LightOnC.glu', utilizing the light-controlled RBP to construct light-controlled transcription factors in C. glutamicum.
Source:
tool establishmentsupports
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum.
Herein, we have established a programmable light-responsive genetic circuit in C. glutamicum, advancing the theory of dynamic regulation based on light signaling.
Source:
tool introductionsupports
LightOnC.glu is a gene expression regulation system in Corynebacterium glutamicum that uses light-controlled RNA-binding proteins to construct light-controlled transcription factors.
Source:
Comparisons
Source-backed strengths
The study established a programmable light-responsive genetic circuit in Corynebacterium glutamicum for dynamic regulation. In application, the engineered synthesis network enabled production of chitin oligosaccharides and chondroitin sulphate oligosaccharides A in Corynebacterium glutamicum for the first time.
Ranked Citations
- 1.