Source 1review2010Current Opinion in Biotechnology
Toolkit/lipoic acid ligase-based intracellular protein labeling
lipoic acid ligase-based intracellular protein labeling
Also known as: intracellular labeling with lipoic acid ligase
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The review focuses on ... new developments in this field such as intracellular labeling with lipoic acid ligase.
Usefulness & Problems
Why this is useful
The review highlights intracellular labeling with lipoic acid ligase as a newer development in protein chemical modification in living cells. It is presented as part of the tag-mediated and site-specific labeling toolkit.; intracellular protein labeling; live-cell chemical modification of proteins
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The review highlights intracellular labeling with lipoic acid ligase as a newer development in protein chemical modification in living cells. It is presented as part of the tag-mediated and site-specific labeling toolkit.
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intracellular protein labeling
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live-cell chemical modification of proteins
Problem solved
It addresses intracellular labeling of proteins in living cells using a ligase-based strategy.; extends protein labeling approaches to intracellular labeling with lipoic acid ligase
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It addresses intracellular labeling of proteins in living cells using a ligase-based strategy.
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extends protein labeling approaches to intracellular labeling with lipoic acid ligase
Problem links
extends protein labeling approaches to intracellular labeling with lipoic acid ligase
LiteratureIt addresses intracellular labeling of proteins in living cells using a ligase-based strategy.
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It addresses intracellular labeling of proteins in living cells using a ligase-based strategy.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
enzyme-mediated covalent labelingintracellular protein labelingtag-mediated site-specific labelingTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: actuator
The abstract supports that this approach depends on a lipoic acid ligase-based intracellular labeling setup, but does not specify the exact tag, substrate, or workflow details.; requires a ligase-based labeling system for intracellular use
The abstract does not show that it overcomes the broader challenge of selectively labeling non-tagged native proteins in complex mixtures.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Intracellular labeling with lipoic acid ligase is presented as a new development in live-cell protein labeling.
new developments in this field such as intracellular labeling with lipoic acid ligase
The review explicitly treats tetracysteine tag and SNAP-tag as central tag-mediated protein labeling methods.
The review focuses on tag-mediated protein labeling methods, such as the tetracysteine tag and SNAP-tag
Approval Evidence
1 source1 linked approval claimfirst-pass slug lipoic-acid-ligase-based-intracellular-protein-labeling
The review focuses on ... new developments in this field such as intracellular labeling with lipoic acid ligase.
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emerging methodsupports
Intracellular labeling with lipoic acid ligase is presented as a new development in live-cell protein labeling.
new developments in this field such as intracellular labeling with lipoic acid ligase
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Comparisons
Source-stated alternatives
The review contrasts this newer ligase-based approach with tetracysteine tag methods, SNAP-tag, and unnatural amino acid incorporation.
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The review contrasts this newer ligase-based approach with tetracysteine tag methods, SNAP-tag, and unnatural amino acid incorporation.
Source-backed strengths
presented as a new development in live-cell protein labeling
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presented as a new development in live-cell protein labeling
Compared with SNAP-tag
The review contrasts this newer ligase-based approach with tetracysteine tag methods, SNAP-tag, and unnatural amino acid incorporation.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a new development in live-cell protein labeling.
Source:
The review contrasts this newer ligase-based approach with tetracysteine tag methods, SNAP-tag, and unnatural amino acid incorporation.
Compared with tetracysteine tag
The review contrasts this newer ligase-based approach with tetracysteine tag methods, SNAP-tag, and unnatural amino acid incorporation.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as a new development in live-cell protein labeling.
Source:
The review contrasts this newer ligase-based approach with tetracysteine tag methods, SNAP-tag, and unnatural amino acid incorporation.
Ranked Citations
- 1.