Source 1primary paper2025MED
Toolkit/Logic-gated AdPROM deploying SrtA-mediated Element Recombination
Logic-gated AdPROM deploying SrtA-mediated Element Recombination
Also known as: LASER, LASER platform
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This Logic-gated AdPROM deploying SrtA-mediated Element Recombination (LASER) platform allows us to expand the possible protein degradation outcomes in mammalian cells using Boolean logic operations depending on the input combinations.
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Published Workflows
Objective: Engineer a logic-gated, switchable targeted protein degradation framework that conditionally controls intracellular protein content in mammalian cells.
Why it works: The framework uses Sortase A cleavage and ligation as a programmable control input to reconfigure bioPROTAC activity, and additional protease gating can alter conditional degradation phenotypes with minimal redesign.
Sortase A-mediated cleavageSortase A-mediated ligationprotease gating layered onto Sortase A controlbioPROTAC-directed targeted protein degradationsynthetic circuit designlogic gatingprotein degradation engineering
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
Degradationligationlogic gatingPhotocleavageproteolytic cleavagetargeted protein degradationTechniques
Computational DesignTarget processes
degradationdiagnosticrecombinationValidation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The platform is flexible enough to modulate the level of multiple native intracellular proteins and may have applications in therapy, diagnostics, and biotechnology.
The flexibility to modulate the level of multiple native intracellular proteins can potentially lead to applications from therapy to diagnostics and biotechnology.
Layering Sortase A input with protease gating allows conditional degradation phenotypes to be adapted with minimal design modifications.
Furthermore, by layering the SrtA input with protease gating, conditional degradation phenotypes can be readily adapted with minimal modifications to the design.
The LASER platform expands possible protein degradation outcomes in mammalian cells using Boolean logic operations based on input combinations.
This Logic-gated AdPROM deploying SrtA-mediated Element Recombination (LASER) platform allows us to expand the possible protein degradation outcomes in mammalian cells using Boolean logic operations depending on the input combinations.
The strategy uses Sortase A cleavage and ligation functions as a control input to direct bioPROTAC activity for switchable targeted protein degradation.
By exploiting both the cleavage and ligation functionalities of Sortase A (SrtA), we present a new strategy utilizing SrtA as the control input to direct bioPROTAC activity for switchable TPD.
The paper introduces a synthetic framework for logic-gated, switchable targeted protein degradation to achieve conditional control of protein content.
To extend the use of TPD beyond just protein attenuation, we introduce a synthetic framework for logic-gated, switchable TPD to achieve conditional control of protein content.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug logic-gated-adprom-deploying-srta-mediated-element-recombination
This Logic-gated AdPROM deploying SrtA-mediated Element Recombination (LASER) platform allows us to expand the possible protein degradation outcomes in mammalian cells using Boolean logic operations depending on the input combinations.
Source:
application scopesupports
The platform is flexible enough to modulate the level of multiple native intracellular proteins and may have applications in therapy, diagnostics, and biotechnology.
The flexibility to modulate the level of multiple native intracellular proteins can potentially lead to applications from therapy to diagnostics and biotechnology.
Source:
design flexibilitysupports
Layering Sortase A input with protease gating allows conditional degradation phenotypes to be adapted with minimal design modifications.
Furthermore, by layering the SrtA input with protease gating, conditional degradation phenotypes can be readily adapted with minimal modifications to the design.
Source:
functional capabilitysupports
The LASER platform expands possible protein degradation outcomes in mammalian cells using Boolean logic operations based on input combinations.
This Logic-gated AdPROM deploying SrtA-mediated Element Recombination (LASER) platform allows us to expand the possible protein degradation outcomes in mammalian cells using Boolean logic operations depending on the input combinations.
Source:
mechanism of actionsupports
The strategy uses Sortase A cleavage and ligation functions as a control input to direct bioPROTAC activity for switchable targeted protein degradation.
By exploiting both the cleavage and ligation functionalities of Sortase A (SrtA), we present a new strategy utilizing SrtA as the control input to direct bioPROTAC activity for switchable TPD.
Source:
tool introductionsupports
The paper introduces a synthetic framework for logic-gated, switchable targeted protein degradation to achieve conditional control of protein content.
To extend the use of TPD beyond just protein attenuation, we introduce a synthetic framework for logic-gated, switchable TPD to achieve conditional control of protein content.
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.