Source 1review2015Frontiers in Molecular Biosciences
Toolkit/LOV-based optogenetic devices
LOV-based optogenetic devices
Also known as: optogenetic tools
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
LOV-based optogenetic devices are engineered tools built from small, light-responsive LOV modules to confer photoregulated control of cellular signaling. The supplied evidence supports their role as a general platform for optogenetic tool construction but does not specify individual device architectures or target proteins.
Usefulness & Problems
Why this is useful
These devices are useful because LOV modules provide a compact light-responsive platform for engineering optical control over signaling processes. The cited source frames them as a means to impart photoregulated control, although quantitative performance or application-specific outcomes are not provided in the supplied evidence.
Problem solved
LOV-based optogenetic devices address the need to control cellular signaling with light rather than constitutive or non-optical inputs. The evidence also indicates that engineering this control is challenged by achieving high sensitivity together with fast on/off kinetics across divergent LOV photocycles.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
Computational DesignTarget processes
signalingInput: Light
Implementation Constraints
The supplied evidence establishes LOV modules as the core light-responsive component used to build these devices. However, it does not provide practical details on cofactors, expression systems, delivery methods, wavelengths, or construct architectures.
Construction of highly sensitive devices with fast on/off kinetics is complicated by divergent photocycle properties among LOV family members. Design and implementation are also hindered by incomplete understanding of how light-driven allosteric conformational changes activate diverse signal transduction domains.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2review2021Small GTPases
Ranked Claims
Further improvements in Rho-control and Rho-detection tools are needed to reveal the role of other Rho GTPases in cytokinesis and to identify molecular mechanisms controlling Rho activity.
Recent optogenetic tools and biosensors that control and detect active Rho have overcome some of these challenges and are helping elucidate the role of RhoA in cytokinesis.
Divergent photocycle properties among LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Further, divergent photocycle properties amongst LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Divergent photocycle properties among LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Further, divergent photocycle properties amongst LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Divergent photocycle properties among LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Further, divergent photocycle properties amongst LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Divergent photocycle properties among LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Further, divergent photocycle properties amongst LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Divergent photocycle properties among LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Further, divergent photocycle properties amongst LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Divergent photocycle properties among LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Further, divergent photocycle properties amongst LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Divergent photocycle properties among LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Further, divergent photocycle properties amongst LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Design and implementation of LOV-based optogenetic devices are hindered by incomplete understanding of how light-driven allosteric conformational changes activate diverse signal transduction domains.
Currently, the design and implementation of these devices is partially hindered by a lack of understanding of how light drives allosteric changes in protein conformation to activate diverse signal transduction domains.
Design and implementation of LOV-based optogenetic devices are hindered by incomplete understanding of how light-driven allosteric conformational changes activate diverse signal transduction domains.
Currently, the design and implementation of these devices is partially hindered by a lack of understanding of how light drives allosteric changes in protein conformation to activate diverse signal transduction domains.
Design and implementation of LOV-based optogenetic devices are hindered by incomplete understanding of how light-driven allosteric conformational changes activate diverse signal transduction domains.
Currently, the design and implementation of these devices is partially hindered by a lack of understanding of how light drives allosteric changes in protein conformation to activate diverse signal transduction domains.
Design and implementation of LOV-based optogenetic devices are hindered by incomplete understanding of how light-driven allosteric conformational changes activate diverse signal transduction domains.
Currently, the design and implementation of these devices is partially hindered by a lack of understanding of how light drives allosteric changes in protein conformation to activate diverse signal transduction domains.
Design and implementation of LOV-based optogenetic devices are hindered by incomplete understanding of how light-driven allosteric conformational changes activate diverse signal transduction domains.
Currently, the design and implementation of these devices is partially hindered by a lack of understanding of how light drives allosteric changes in protein conformation to activate diverse signal transduction domains.
Design and implementation of LOV-based optogenetic devices are hindered by incomplete understanding of how light-driven allosteric conformational changes activate diverse signal transduction domains.
Currently, the design and implementation of these devices is partially hindered by a lack of understanding of how light drives allosteric changes in protein conformation to activate diverse signal transduction domains.
Design and implementation of LOV-based optogenetic devices are hindered by incomplete understanding of how light-driven allosteric conformational changes activate diverse signal transduction domains.
Currently, the design and implementation of these devices is partially hindered by a lack of understanding of how light drives allosteric changes in protein conformation to activate diverse signal transduction domains.
LOV modules are presented as a platform for constructing optogenetic tools.
The small, light responsive LOV modules offer a novel platform for the construction of optogenetic tools.
LOV modules are presented as a platform for constructing optogenetic tools.
The small, light responsive LOV modules offer a novel platform for the construction of optogenetic tools.
LOV modules are presented as a platform for constructing optogenetic tools.
The small, light responsive LOV modules offer a novel platform for the construction of optogenetic tools.
LOV modules are presented as a platform for constructing optogenetic tools.
The small, light responsive LOV modules offer a novel platform for the construction of optogenetic tools.
LOV modules are presented as a platform for constructing optogenetic tools.
The small, light responsive LOV modules offer a novel platform for the construction of optogenetic tools.
LOV modules are presented as a platform for constructing optogenetic tools.
The small, light responsive LOV modules offer a novel platform for the construction of optogenetic tools.
LOV modules are presented as a platform for constructing optogenetic tools.
The small, light responsive LOV modules offer a novel platform for the construction of optogenetic tools.
The review focuses on tuning LOV domain chemistry and signal transduction to improve optogenetic tools.
In the present review we discuss the history of LOV domain research with primary emphasis on tuning LOV domain chemistry and signal transduction to allow for improved optogenetic tools.
The review focuses on tuning LOV domain chemistry and signal transduction to improve optogenetic tools.
In the present review we discuss the history of LOV domain research with primary emphasis on tuning LOV domain chemistry and signal transduction to allow for improved optogenetic tools.
The review focuses on tuning LOV domain chemistry and signal transduction to improve optogenetic tools.
In the present review we discuss the history of LOV domain research with primary emphasis on tuning LOV domain chemistry and signal transduction to allow for improved optogenetic tools.
The review focuses on tuning LOV domain chemistry and signal transduction to improve optogenetic tools.
In the present review we discuss the history of LOV domain research with primary emphasis on tuning LOV domain chemistry and signal transduction to allow for improved optogenetic tools.
The review focuses on tuning LOV domain chemistry and signal transduction to improve optogenetic tools.
In the present review we discuss the history of LOV domain research with primary emphasis on tuning LOV domain chemistry and signal transduction to allow for improved optogenetic tools.
The review focuses on tuning LOV domain chemistry and signal transduction to improve optogenetic tools.
In the present review we discuss the history of LOV domain research with primary emphasis on tuning LOV domain chemistry and signal transduction to allow for improved optogenetic tools.
The review focuses on tuning LOV domain chemistry and signal transduction to improve optogenetic tools.
In the present review we discuss the history of LOV domain research with primary emphasis on tuning LOV domain chemistry and signal transduction to allow for improved optogenetic tools.
Approval Evidence
2 sources6 linked approval claimsfirst-pass slugs lov-based-optogenetic-devices, optogenetic-tools-for-rho-gtpase-control
The recent generation of optogenetic tools and biosensors that control and detect active Rho has overcome some of these challenges and is helping to elucidate the role of RhoA in cytokinesis.
Source:
LOV-based optogenetic devices: light-driven modules to impart photoregulated control of cellular signaling... The small, light responsive LOV modules offer a novel platform for the construction of optogenetic tools.
Source:
knowledge gapsupports
Further improvements in Rho-control and Rho-detection tools are needed to reveal the role of other Rho GTPases in cytokinesis and to identify molecular mechanisms controlling Rho activity.
Source:
review summarysupports
Recent optogenetic tools and biosensors that control and detect active Rho have overcome some of these challenges and are helping elucidate the role of RhoA in cytokinesis.
Source:
engineering constraintsupports
Divergent photocycle properties among LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Further, divergent photocycle properties amongst LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Source:
engineering limitationsupports
Design and implementation of LOV-based optogenetic devices are hindered by incomplete understanding of how light-driven allosteric conformational changes activate diverse signal transduction domains.
Currently, the design and implementation of these devices is partially hindered by a lack of understanding of how light drives allosteric changes in protein conformation to activate diverse signal transduction domains.
Source:
platform potentialsupports
LOV modules are presented as a platform for constructing optogenetic tools.
The small, light responsive LOV modules offer a novel platform for the construction of optogenetic tools.
Source:
review focusneutral
The review focuses on tuning LOV domain chemistry and signal transduction to improve optogenetic tools.
In the present review we discuss the history of LOV domain research with primary emphasis on tuning LOV domain chemistry and signal transduction to allow for improved optogenetic tools.
Source:
Comparisons
Source-backed strengths
A key strength supported by the evidence is that LOV modules are small and light responsive, making them a versatile platform for optogenetic engineering. The source specifically positions them for photoregulated control of cellular signaling, but no comparative benchmarks or validation data are included here.
Source:
Further, divergent photocycle properties amongst LOV family members complicate construction of highly sensitive devices with fast on/off kinetics.
Source:
Currently, the design and implementation of these devices is partially hindered by a lack of understanding of how light drives allosteric changes in protein conformation to activate diverse signal transduction domains.
Ranked Citations
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