Source 1review2023Journal of Molecular Biology
Toolkit/LOV-based optogenetic tool
LOV-based optogenetic tool
Also known as: LOV-based optogenetic tools
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
LOV-based optogenetic tools are multi-component light-responsive systems built from Light-Oxygen-Voltage sensing domains. These domains function as versatile photoreceptors involved in cellular signaling and environmental adaptation across kingdoms of life, and their continued development is intended to expand optical control of biological systems.
Usefulness & Problems
Why this is useful
These tools are useful because they provide a route to optical control using naturally occurring LOV photoreceptor modules. The cited literature states that their ongoing development has the potential to revolutionize the study of biological systems and may support future therapeutic strategies, but specific application benchmarks are not provided in the supplied evidence.
Source:
Moreover, these domains have been identified across all kingdoms of life. LOV domains are versatile photoreceptors that play critical roles in cellular signaling and environmental adaptation
Problem solved
LOV-based optogenetic tools address the need for genetically encoded, light-responsive control elements for probing and manipulating biological systems. The supplied evidence supports this general role, but it does not specify particular target pathways, cell types, or experimental bottlenecks solved by any one implementation.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
Implementation details are sparse in the provided evidence. It is known only that these tools are based on LOV sensing domains and use light as the input modality; the sources do not specify cofactors, host systems, construct architectures, or delivery methods.
The supplied evidence is high level and does not report quantitative performance metrics such as dynamic range, kinetics, wavelength specificity, reversibility, or background activity. It also does not identify specific constructs, validation contexts, or independent demonstrations of a particular LOV-based switch.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
LOV domains are versatile photoreceptors involved in cellular signaling and environmental adaptation across kingdoms of life.
Moreover, these domains have been identified across all kingdoms of life. LOV domains are versatile photoreceptors that play critical roles in cellular signaling and environmental adaptation
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
LOV domains use flavin nucleotides as cofactors and undergo blue-light-induced structural rearrangements that activate an effector domain.
LOV domains utilize flavin nucleotides as co-factors and undergo structural rearrangements upon exposure to blue light, which activates an effector domain that executes the final output of the photoreaction.
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
Approval Evidence
1 source3 linked approval claimsfirst-pass slug lov-based-optogenetic-tool
The ongoing development of LOV-based optogenetic tools... has the potential to revolutionize the study of biological systems...
Source:
development driversupports
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
Source:
future potentialsupports
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
Source:
optogenetic utilitysupports
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
Source:
Comparisons
Source-backed strengths
A key strength is that LOV domains are broadly distributed and naturally function in cellular signaling and environmental adaptation across kingdoms of life. The literature also indicates that tool development is being accelerated by advances in structural biology, spectroscopy, computational methods, and synthetic biology, suggesting a strong foundation for continued engineering.
Ranked Citations
- 1.