Source 1primary paper2022G3 Genes Genomes Genetics
Toolkit/LOV-LexA
LOV-LexA
Also known as: light-gated LOV-LexA, LOV-LexA tool
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
LOV-LexA is a light-gated LexA-based expression system for Drosophila that fuses the bacterial LexA transcription factor to a plant-derived LOV photosensitive domain and a fluorescent protein. Blue light uncages a nuclear localization signal, drives nuclear translocation, and initiates LexAop transgene expression with spatial and temporal control.
Usefulness & Problems
Why this is useful
This tool provides optical control over transgene expression, enabling spatially and temporally restricted activation of LexAop reporters or effectors in Drosophila tissues. It is reported to be compatible with GAL4 and Split-GAL4 drivers, adding an intersectional genetics layer for light-controlled access to specific cells across flies.
Source:
LOV-LexA enables spatial and temporal control of expression of transgenes under LexAop sequences in larval fat body and pupal and adult neurons with blue light.
Source:
To access the same cells within a given expression pattern consistently across fruit flies, we developed the light-gated expression system LOV-LexA.
Problem solved
LOV-LexA addresses the need for noninvasive, light-dependent control of gene expression in defined cells and time windows. The reported system enables blue-light-triggered LexAop expression in larval fat body and in pupal and adult neurons.
Source:
To access the same cells within a given expression pattern consistently across fruit flies, we developed the light-gated expression system LOV-LexA.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
conformational uncagingConformational Uncaginglight-induced nuclear translocationtranscriptional activationTechniques
No technique tags yet.
Target processes
localizationtranscriptionInput: Light
Implementation Constraints
The construct combines LexA with a plant-derived LOV photosensitive domain and a fluorescent protein, and its function depends on blue light exposure. The reported output is LexAop transgene expression in Drosophila, and the system is described as usable with GAL4 and Split-GAL4 drivers.
The supplied evidence is limited to a single 2022 publication and does not provide quantitative performance metrics such as induction kinetics, dynamic range, leakiness, or reversibility. Practical constraints such as light penetration, phototoxicity, and construct size are not described in the provided evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
LOV-LexA is ready to use with GAL4 and Split-GAL4 drivers and provides a layer of intersectional genetics for light-controlled access to specific cells across flies.
The LOV-LexA tool is ready to use with GAL4 and Split-GAL4 drivers in its current form and constitutes another layer of intersectional genetics that provides light-controlled genetic access to specific cells across flies.
LOV-LexA is ready to use with GAL4 and Split-GAL4 drivers and provides a layer of intersectional genetics for light-controlled access to specific cells across flies.
The LOV-LexA tool is ready to use with GAL4 and Split-GAL4 drivers in its current form and constitutes another layer of intersectional genetics that provides light-controlled genetic access to specific cells across flies.
LOV-LexA is ready to use with GAL4 and Split-GAL4 drivers and provides a layer of intersectional genetics for light-controlled access to specific cells across flies.
The LOV-LexA tool is ready to use with GAL4 and Split-GAL4 drivers in its current form and constitutes another layer of intersectional genetics that provides light-controlled genetic access to specific cells across flies.
LOV-LexA is ready to use with GAL4 and Split-GAL4 drivers and provides a layer of intersectional genetics for light-controlled access to specific cells across flies.
The LOV-LexA tool is ready to use with GAL4 and Split-GAL4 drivers in its current form and constitutes another layer of intersectional genetics that provides light-controlled genetic access to specific cells across flies.
LOV-LexA is ready to use with GAL4 and Split-GAL4 drivers and provides a layer of intersectional genetics for light-controlled access to specific cells across flies.
The LOV-LexA tool is ready to use with GAL4 and Split-GAL4 drivers in its current form and constitutes another layer of intersectional genetics that provides light-controlled genetic access to specific cells across flies.
LOV-LexA is ready to use with GAL4 and Split-GAL4 drivers and provides a layer of intersectional genetics for light-controlled access to specific cells across flies.
The LOV-LexA tool is ready to use with GAL4 and Split-GAL4 drivers in its current form and constitutes another layer of intersectional genetics that provides light-controlled genetic access to specific cells across flies.
LOV-LexA is ready to use with GAL4 and Split-GAL4 drivers and provides a layer of intersectional genetics for light-controlled access to specific cells across flies.
The LOV-LexA tool is ready to use with GAL4 and Split-GAL4 drivers in its current form and constitutes another layer of intersectional genetics that provides light-controlled genetic access to specific cells across flies.
LOV-LexA enables spatial and temporal control of LexAop transgene expression with blue light in larval fat body and pupal and adult neurons.
LOV-LexA enables spatial and temporal control of expression of transgenes under LexAop sequences in larval fat body and pupal and adult neurons with blue light.
LOV-LexA enables spatial and temporal control of LexAop transgene expression with blue light in larval fat body and pupal and adult neurons.
LOV-LexA enables spatial and temporal control of expression of transgenes under LexAop sequences in larval fat body and pupal and adult neurons with blue light.
LOV-LexA enables spatial and temporal control of LexAop transgene expression with blue light in larval fat body and pupal and adult neurons.
LOV-LexA enables spatial and temporal control of expression of transgenes under LexAop sequences in larval fat body and pupal and adult neurons with blue light.
LOV-LexA enables spatial and temporal control of LexAop transgene expression with blue light in larval fat body and pupal and adult neurons.
LOV-LexA enables spatial and temporal control of expression of transgenes under LexAop sequences in larval fat body and pupal and adult neurons with blue light.
LOV-LexA enables spatial and temporal control of LexAop transgene expression with blue light in larval fat body and pupal and adult neurons.
LOV-LexA enables spatial and temporal control of expression of transgenes under LexAop sequences in larval fat body and pupal and adult neurons with blue light.
LOV-LexA enables spatial and temporal control of LexAop transgene expression with blue light in larval fat body and pupal and adult neurons.
LOV-LexA enables spatial and temporal control of expression of transgenes under LexAop sequences in larval fat body and pupal and adult neurons with blue light.
LOV-LexA enables spatial and temporal control of LexAop transgene expression with blue light in larval fat body and pupal and adult neurons.
LOV-LexA enables spatial and temporal control of expression of transgenes under LexAop sequences in larval fat body and pupal and adult neurons with blue light.
LOV-LexA combines the bacterial LexA transcription factor with a plant-derived LOV photosensitive domain and a fluorescent protein, and blue light exposure uncages a nuclear localization signal leading to nuclear translocation and transcription initiation.
We combined the bacterial LexA transcription factor with the plant-derived light, oxygen, or voltage photosensitive domain and a fluorescent protein. Exposure to blue light uncages a nuclear localizing signal in the C-terminal of the light, oxygen, or voltage domain and leads to the translocation of LOV-LexA to the nucleus, with the subsequent initiation of transcription.
LOV-LexA combines the bacterial LexA transcription factor with a plant-derived LOV photosensitive domain and a fluorescent protein, and blue light exposure uncages a nuclear localization signal leading to nuclear translocation and transcription initiation.
We combined the bacterial LexA transcription factor with the plant-derived light, oxygen, or voltage photosensitive domain and a fluorescent protein. Exposure to blue light uncages a nuclear localizing signal in the C-terminal of the light, oxygen, or voltage domain and leads to the translocation of LOV-LexA to the nucleus, with the subsequent initiation of transcription.
LOV-LexA combines the bacterial LexA transcription factor with a plant-derived LOV photosensitive domain and a fluorescent protein, and blue light exposure uncages a nuclear localization signal leading to nuclear translocation and transcription initiation.
We combined the bacterial LexA transcription factor with the plant-derived light, oxygen, or voltage photosensitive domain and a fluorescent protein. Exposure to blue light uncages a nuclear localizing signal in the C-terminal of the light, oxygen, or voltage domain and leads to the translocation of LOV-LexA to the nucleus, with the subsequent initiation of transcription.
LOV-LexA combines the bacterial LexA transcription factor with a plant-derived LOV photosensitive domain and a fluorescent protein, and blue light exposure uncages a nuclear localization signal leading to nuclear translocation and transcription initiation.
We combined the bacterial LexA transcription factor with the plant-derived light, oxygen, or voltage photosensitive domain and a fluorescent protein. Exposure to blue light uncages a nuclear localizing signal in the C-terminal of the light, oxygen, or voltage domain and leads to the translocation of LOV-LexA to the nucleus, with the subsequent initiation of transcription.
LOV-LexA combines the bacterial LexA transcription factor with a plant-derived LOV photosensitive domain and a fluorescent protein, and blue light exposure uncages a nuclear localization signal leading to nuclear translocation and transcription initiation.
We combined the bacterial LexA transcription factor with the plant-derived light, oxygen, or voltage photosensitive domain and a fluorescent protein. Exposure to blue light uncages a nuclear localizing signal in the C-terminal of the light, oxygen, or voltage domain and leads to the translocation of LOV-LexA to the nucleus, with the subsequent initiation of transcription.
LOV-LexA combines the bacterial LexA transcription factor with a plant-derived LOV photosensitive domain and a fluorescent protein, and blue light exposure uncages a nuclear localization signal leading to nuclear translocation and transcription initiation.
We combined the bacterial LexA transcription factor with the plant-derived light, oxygen, or voltage photosensitive domain and a fluorescent protein. Exposure to blue light uncages a nuclear localizing signal in the C-terminal of the light, oxygen, or voltage domain and leads to the translocation of LOV-LexA to the nucleus, with the subsequent initiation of transcription.
LOV-LexA combines the bacterial LexA transcription factor with a plant-derived LOV photosensitive domain and a fluorescent protein, and blue light exposure uncages a nuclear localization signal leading to nuclear translocation and transcription initiation.
We combined the bacterial LexA transcription factor with the plant-derived light, oxygen, or voltage photosensitive domain and a fluorescent protein. Exposure to blue light uncages a nuclear localizing signal in the C-terminal of the light, oxygen, or voltage domain and leads to the translocation of LOV-LexA to the nucleus, with the subsequent initiation of transcription.
The authors developed the light-gated expression system LOV-LexA to access the same cells within a given expression pattern consistently across fruit flies.
To access the same cells within a given expression pattern consistently across fruit flies, we developed the light-gated expression system LOV-LexA.
The authors developed the light-gated expression system LOV-LexA to access the same cells within a given expression pattern consistently across fruit flies.
To access the same cells within a given expression pattern consistently across fruit flies, we developed the light-gated expression system LOV-LexA.
The authors developed the light-gated expression system LOV-LexA to access the same cells within a given expression pattern consistently across fruit flies.
To access the same cells within a given expression pattern consistently across fruit flies, we developed the light-gated expression system LOV-LexA.
The authors developed the light-gated expression system LOV-LexA to access the same cells within a given expression pattern consistently across fruit flies.
To access the same cells within a given expression pattern consistently across fruit flies, we developed the light-gated expression system LOV-LexA.
The authors developed the light-gated expression system LOV-LexA to access the same cells within a given expression pattern consistently across fruit flies.
To access the same cells within a given expression pattern consistently across fruit flies, we developed the light-gated expression system LOV-LexA.
The authors developed the light-gated expression system LOV-LexA to access the same cells within a given expression pattern consistently across fruit flies.
To access the same cells within a given expression pattern consistently across fruit flies, we developed the light-gated expression system LOV-LexA.
The authors developed the light-gated expression system LOV-LexA to access the same cells within a given expression pattern consistently across fruit flies.
To access the same cells within a given expression pattern consistently across fruit flies, we developed the light-gated expression system LOV-LexA.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug lov-lexa
we developed the light-gated expression system LOV-LexA
Source:
compatibilitysupports
LOV-LexA is ready to use with GAL4 and Split-GAL4 drivers and provides a layer of intersectional genetics for light-controlled access to specific cells across flies.
The LOV-LexA tool is ready to use with GAL4 and Split-GAL4 drivers in its current form and constitutes another layer of intersectional genetics that provides light-controlled genetic access to specific cells across flies.
Source:
functional capabilitysupports
LOV-LexA enables spatial and temporal control of LexAop transgene expression with blue light in larval fat body and pupal and adult neurons.
LOV-LexA enables spatial and temporal control of expression of transgenes under LexAop sequences in larval fat body and pupal and adult neurons with blue light.
Source:
mechanismsupports
LOV-LexA combines the bacterial LexA transcription factor with a plant-derived LOV photosensitive domain and a fluorescent protein, and blue light exposure uncages a nuclear localization signal leading to nuclear translocation and transcription initiation.
We combined the bacterial LexA transcription factor with the plant-derived light, oxygen, or voltage photosensitive domain and a fluorescent protein. Exposure to blue light uncages a nuclear localizing signal in the C-terminal of the light, oxygen, or voltage domain and leads to the translocation of LOV-LexA to the nucleus, with the subsequent initiation of transcription.
Source:
tool developmentsupports
The authors developed the light-gated expression system LOV-LexA to access the same cells within a given expression pattern consistently across fruit flies.
To access the same cells within a given expression pattern consistently across fruit flies, we developed the light-gated expression system LOV-LexA.
Source:
Comparisons
Source-backed strengths
The system was specifically developed for spatial and temporal control of expression with blue light. Reported validation includes function in multiple Drosophila contexts, including larval fat body and pupal and adult neurons, and compatibility with GAL4 and Split-GAL4 driver frameworks.
Ranked Citations
- 1.