Source 1primary paper2022Photochemical & Photobiological Sciences
Toolkit/LOV photoreceptor
LOV photoreceptor
Also known as: Light, oxygen, voltage (LOV) photoreceptor, LOV photoreceptors
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
LOV photoreceptors are modular light-sensing protein domains that have been broadly used as sensor domains in optogenetic tools. Evidence indicates that mutations in a conserved hydrophobic pocket can alter the lifetime of the photo-adduct signaling state, thereby tuning sensor kinetics and steady-state on/off equilibria.
Usefulness & Problems
Why this is useful
These domains are useful because their modularity has enabled broad deployment as sensory inputs in optogenetic tool construction. The cited evidence further indicates that conserved-pocket mutations can be used for dark-recovery tuning, providing a route to adjust signaling-state persistence and response timing.
Source:
Given the conserved nature of the corresponding structural region, the here identified mutations should find application in dark-recovery tuning of optogenetic tools and LOV photoreceptors, alike.
Source:
LOV photoreceptors are widely distributed throughout all kingdoms of life, and have in recent years, due to their modular nature, been broadly used as sensor domains for the construction of optogenetic tools.
Problem solved
LOV photoreceptors help solve the problem of coupling light input to engineered signaling outputs in optogenetic systems. The specific engineering problem addressed by the cited study is how to tune dark recovery and on/off kinetics by changing the adduct-state lifetime through mutations in a conserved hydrophobic pocket.
Source:
Given the conserved nature of the corresponding structural region, the here identified mutations should find application in dark-recovery tuning of optogenetic tools and LOV photoreceptors, alike.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Mechanisms
dark recovery tuningkinetic tuning of signaling-state lifetimelight sensing via a photo-adduct signaling stateTechniques
Structural CharacterizationTarget processes
signalingInput: Light
Implementation Constraints
Implementation evidence supports use of LOV domains as modular sensor components in domain-fused optogenetic tools. Practical details such as chromophore requirements, construct architecture, expression systems, and delivery methods are not specified in the supplied evidence.
The supplied evidence focuses on kinetic tuning of the LOV signaling state and does not provide quantitative performance metrics, specific mutation sets, or direct comparisons among optogenetic implementations. It also does not describe downstream effector domains, illumination parameters, or validation in particular cellular or organismal contexts.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The identified conserved-pocket mutations should find application in dark-recovery tuning of optogenetic tools and LOV photoreceptors.
Given the conserved nature of the corresponding structural region, the here identified mutations should find application in dark-recovery tuning of optogenetic tools and LOV photoreceptors, alike.
Mutations that alter the lifetime of the photo-adduct signaling state can tune LOV sensor on/off kinetics and steady-state on/off equilibria.
Mutations that alter the lifetime of the photo-adduct signaling state represent a convenient handle to tune LOV sensor on/off kinetics and, thus, steady-state on/off equilibria of the photoreceptor (or optogenetic switch).
LOV photoreceptors have been broadly used as sensor domains for the construction of optogenetic tools.
LOV photoreceptors are widely distributed throughout all kingdoms of life, and have in recent years, due to their modular nature, been broadly used as sensor domains for the construction of optogenetic tools.
A conserved hydrophobic pocket has mutations with strong impact on adduct-state lifetime across different LOV photoreceptor families.
we identify a conserved hydrophobic pocket for which mutations have a strong impact on the adduct-state lifetime across different LOV photoreceptor families
Approval Evidence
1 source4 linked approval claimsfirst-pass slug lov-photoreceptor
Light, oxygen, voltage (LOV) photoreceptors are widely distributed throughout all kingdoms of life, and have in recent years, due to their modular nature, been broadly used as sensor domains for the construction of optogenetic tools.
Source:
application potentialsupports
The identified conserved-pocket mutations should find application in dark-recovery tuning of optogenetic tools and LOV photoreceptors.
Given the conserved nature of the corresponding structural region, the here identified mutations should find application in dark-recovery tuning of optogenetic tools and LOV photoreceptors, alike.
Source:
engineering principlesupports
Mutations that alter the lifetime of the photo-adduct signaling state can tune LOV sensor on/off kinetics and steady-state on/off equilibria.
Mutations that alter the lifetime of the photo-adduct signaling state represent a convenient handle to tune LOV sensor on/off kinetics and, thus, steady-state on/off equilibria of the photoreceptor (or optogenetic switch).
Source:
functional rolesupports
LOV photoreceptors have been broadly used as sensor domains for the construction of optogenetic tools.
LOV photoreceptors are widely distributed throughout all kingdoms of life, and have in recent years, due to their modular nature, been broadly used as sensor domains for the construction of optogenetic tools.
Source:
structure functionsupports
A conserved hydrophobic pocket has mutations with strong impact on adduct-state lifetime across different LOV photoreceptor families.
we identify a conserved hydrophobic pocket for which mutations have a strong impact on the adduct-state lifetime across different LOV photoreceptor families
Source:
Comparisons
Source-backed strengths
A key strength is broad applicability as modular sensor domains across optogenetic constructs. The evidence also supports a structure-function principle in which mutations in a conserved hydrophobic pocket strongly impact adduct-state lifetime across different LOV photoreceptor families, enabling kinetic tuning of signaling behavior.
Source:
Mutations that alter the lifetime of the photo-adduct signaling state represent a convenient handle to tune LOV sensor on/off kinetics and, thus, steady-state on/off equilibria of the photoreceptor (or optogenetic switch).
Ranked Citations
- 1.
Derived from 4 linked claims. Example evidence: Given the conserved nature of the corresponding structural region, the here identified mutations should find application in dark-recovery tuning of optogenetic tools and LOV photoreceptors, alike.