Source 1primary paper2016Methods in enzymology on CD-ROM/Methods in enzymology
Toolkit/LOV2-based photoswitches
LOV2-based photoswitches
Also known as: optogenetic switches, photoswitches
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
LOV2-based photoswitches are optogenetic switches engineered from the LOV2 photoreceptor domain to control biological activities with light. They repurpose endogenous light-induced conformational changes in LOV2 to generate new cellular outputs and have been developed on the basis of detailed biophysical characterization of the isolated domain.
Usefulness & Problems
Why this is useful
These switches are useful for optical control of cellular processes and have contributed to the study of previously intractable biological phenomena. The cited work also provides optimized validation and improvement workflows, including fluorescent polarization, phage display, and microscopy protocols for newly designed photoswitches.
Source:
Cellular optogenetic switches, a novel class of biological tools, have improved our understanding of biological phenomena that were previously intractable.
Problem solved
LOV2-based photoswitches address the problem of converting light input into controllable regulation of non-native biological outputs. More specifically, they solve the engineering challenge of repurposing photoreceptor conformational responses for targeted cellular control.
Source:
Cellular optogenetic switches, a novel class of biological tools, have improved our understanding of biological phenomena that were previously intractable.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Target processes
recombinationInput: Light
Implementation Constraints
LOV2-based photoswitches are built from photoreceptor elements originally borrowed from plant and bacterial systems, with LOV2 serving as the engineered light-responsive module. Practical implementation evidence in the cited work includes protocols optimized for fluorescent polarization, phage display, and microscopy to validate, improve, and apply newly designed photoswitches, but construct architecture and cofactor requirements are not specified in the supplied evidence.
The supplied evidence does not report quantitative performance metrics, specific dynamic range, kinetics, wavelength dependence, or recombination-specific validation for a particular LOV2 switch. It also does not establish independent replication of any one implementation beyond the cited engineering and methods-focused sources.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2020UNC Libraries
Ranked Claims
Cellular optogenetic switches have improved understanding of previously intractable biological phenomena.
Cellular optogenetic switches, a novel class of biological tools, have improved our understanding of biological phenomena that were previously intractable.
Optogenetic switches designed to date are based on borrowed elements from plant and bacterial photoreceptors.
they are all based on borrowed elements from plant and bacterial photoreceptors
Thorough biophysical characterization of the isolated LOV2 domain has created a strong foundation for engineering photoswitches.
its thorough biophysical characterization as an isolated domain has created a strong foundation for engineering of photoswitches
Optogenetic switches exploit endogenous light-induced photoreceptor conformational changes and repurpose their effects to different biological phenomena.
each of the optogenetic switches designed to date exploits the endogenous light induced change in photoreceptor conformation while repurposing its effect to target a different biological phenomena
The chapter provides protocols for fluorescent polarization, phage display, and microscopy optimized for validating, improving, and using newly designed photoswitches.
we provide protocols for assays including fluorescent polarization, phage display, and microscopy that are optimized for validating, improving, and using newly designed photoswitches
The chapter provides protocols for fluorescent polarization, phage display, and microscopy optimized for validating, improving, and using newly designed photoswitches.
we provide protocols for assays including fluorescent polarization, phage display, and microscopy that are optimized for validating, improving, and using newly designed photoswitches
The chapter provides protocols for fluorescent polarization, phage display, and microscopy optimized for validating, improving, and using newly designed photoswitches.
we provide protocols for assays including fluorescent polarization, phage display, and microscopy that are optimized for validating, improving, and using newly designed photoswitches
The chapter provides protocols for fluorescent polarization, phage display, and microscopy optimized for validating, improving, and using newly designed photoswitches.
we provide protocols for assays including fluorescent polarization, phage display, and microscopy that are optimized for validating, improving, and using newly designed photoswitches
The chapter provides protocols for fluorescent polarization, phage display, and microscopy optimized for validating, improving, and using newly designed photoswitches.
we provide protocols for assays including fluorescent polarization, phage display, and microscopy that are optimized for validating, improving, and using newly designed photoswitches
The chapter provides protocols for fluorescent polarization, phage display, and microscopy optimized for validating, improving, and using newly designed photoswitches.
we provide protocols for assays including fluorescent polarization, phage display, and microscopy that are optimized for validating, improving, and using newly designed photoswitches
The chapter provides protocols for fluorescent polarization, phage display, and microscopy optimized for validating, improving, and using newly designed photoswitches.
we provide protocols for assays including fluorescent polarization, phage display, and microscopy that are optimized for validating, improving, and using newly designed photoswitches
This paper concerns the engineering and application of LOV2-based photoswitches.
This paper concerns the engineering and application of LOV2-based photoswitches.
This paper concerns the engineering and application of LOV2-based photoswitches.
This paper concerns the engineering and application of LOV2-based photoswitches.
This paper concerns the engineering and application of LOV2-based photoswitches.
This paper concerns the engineering and application of LOV2-based photoswitches.
This paper concerns the engineering and application of LOV2-based photoswitches.
Approval Evidence
2 sources6 linked approval claimsfirst-pass slug lov2-based-photoswitches
Engineering and Application of LOV2-based Photoswitches
Source:
Engineering and Application of LOV2-Based Photoswitches
Source:
application scopesupports
Cellular optogenetic switches have improved understanding of previously intractable biological phenomena.
Cellular optogenetic switches, a novel class of biological tools, have improved our understanding of biological phenomena that were previously intractable.
Source:
design basissupports
Optogenetic switches designed to date are based on borrowed elements from plant and bacterial photoreceptors.
they are all based on borrowed elements from plant and bacterial photoreceptors
Source:
engineering foundationsupports
Thorough biophysical characterization of the isolated LOV2 domain has created a strong foundation for engineering photoswitches.
its thorough biophysical characterization as an isolated domain has created a strong foundation for engineering of photoswitches
Source:
mechanismsupports
Optogenetic switches exploit endogenous light-induced photoreceptor conformational changes and repurpose their effects to different biological phenomena.
each of the optogenetic switches designed to date exploits the endogenous light induced change in photoreceptor conformation while repurposing its effect to target a different biological phenomena
Source:
protocol availabilitysupports
The chapter provides protocols for fluorescent polarization, phage display, and microscopy optimized for validating, improving, and using newly designed photoswitches.
we provide protocols for assays including fluorescent polarization, phage display, and microscopy that are optimized for validating, improving, and using newly designed photoswitches
Source:
engineering and application topicsupports
This paper concerns the engineering and application of LOV2-based photoswitches.
Source:
Comparisons
Source-backed strengths
A key strength is that their engineering is supported by thorough biophysical characterization of the isolated LOV2 domain. The source also indicates an established methodological framework for validation and optimization through functional assays such as fluorescent polarization and microscopy, as well as phage display-based improvement.
Source:
its thorough biophysical characterization as an isolated domain has created a strong foundation for engineering of photoswitches
Source:
This paper concerns the engineering and application of LOV2-based photoswitches.
Ranked Citations
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