Source 1primary paper2013Chemistry & Biology
Toolkit/LOV2 domain-based optogenetic tool
LOV2 domain-based optogenetic tool
Also known as: LOV2 domain-based optogenetic tool
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The LOV2 domain-based optogenetic tool is a light-responsive protein-domain system reported to control protein degradation and cellular function. The supplied evidence identifies a LOV2-based optogenetic platform but does not specify the exact construct architecture or experimental context.
Usefulness & Problems
Why this is useful
This tool is useful because it enables light-dependent control over protein degradation, linking optical input to regulation of cellular function. The available evidence supports this general use case, but does not provide details on dynamic range, kinetics, or cell-type scope.
Source:
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Problem solved
It addresses the problem of controlling protein degradation with light in order to modulate cellular function. The evidence does not further define the specific biological bottleneck, target protein class, or application setting.
Problem links
Need conditional protein clearance
DerivedThis tool is a LOV2 domain-based optogenetic system reported to control protein degradation and cellular function using light. The supplied evidence identifies the platform at the level of a light-responsive LOV2-domain tool but does not provide construct-level or assay-level details.
Need precise spatiotemporal control with light input
DerivedThis tool is a LOV2 domain-based optogenetic system reported to control protein degradation and cellular function using light. The supplied evidence identifies the platform at the level of a light-responsive LOV2-domain tool but does not provide construct-level or assay-level details.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Techniques
No technique tags yet.
Target processes
degradationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: actuatoroperating role: regulatorswitch architecture: single chain
Implementation appears to involve a LOV2 domain-based optogenetic design for light control of protein degradation. However, the evidence does not specify fusion partners, cofactors, expression system, delivery method, or construct design requirements.
The supplied evidence is limited to the article title and a high-level functional claim. It does not report the molecular design, illumination parameters, degradation pathway components, organism, assay format, or extent of validation.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Section: title
Approval Evidence
1 source1 linked approval claimfirst-pass slug lov2-domain-based-optogenetic-tool
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Source:
tool functionsupports
A LOV2 domain-based optogenetic tool is presented to control protein degradation and cellular function.
A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function
Source:
Comparisons
Source-backed strengths
A key strength supported by the source is that the system is LOV2 domain-based and optogenetic, indicating genetically encoded light responsiveness for control of protein degradation. The evidence also states that cellular function can be controlled, but no quantitative validation or comparative performance data are provided.
Compared with Jalpha helix
LOV2 domain-based optogenetic tool and Jalpha helix address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation; same primary input modality: light
Compared with photo-N-degron
LOV2 domain-based optogenetic tool and photo-N-degron address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation; same primary input modality: light
Compared with photosensitive degron
LOV2 domain-based optogenetic tool and photosensitive degron address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.