Source 1primary paper2006IEEE Transactions on NanoBioscience
Toolkit/LOV2 domain from Avena sativa phot1 phototropin
LOV2 domain from Avena sativa phot1 phototropin
Also known as: Avena sativa (oat) phot1 phototropin LOV2 domain, LOV2
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The LOV2 domain from Avena sativa phot1 phototropin is a blue-light-responsive protein domain analyzed as an all-optical switching medium. In the cited theoretical study, a 442 nm pulsed pump modulates transmission of a 660 nm continuous-wave probe through LOV2 via nonlinear intensity-induced excited-state absorption.
Usefulness & Problems
Why this is useful
This domain is useful as a protein-based optical switching element for modulating one light beam with another in an all-optical format. The cited work further used its switching characteristics to design NOT, NOR, and NAND optical logic gates.
Problem solved
It addresses the problem of achieving all-optical signal switching and logic operations using a biological chromoprotein medium rather than an electronically controlled component. The study specifically analyzes how LOV2 can convert blue-light pump input into modulation of red probe transmission.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Techniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The analyzed configuration uses a 442 nm pulsed pump beam and a 660 nm continuous-wave probe beam. Reported full modulation was obtained for a 1 mM LOV2 sample with 5.5 mm optical path length at 50 kW/cm2 pump intensity, and construct expression or delivery details are not provided in the supplied evidence.
The evidence provided is limited to a theoretical analysis from a single cited study, and no independent experimental replication is described here. Performance is parameter-sensitive, depending on concentration, L-state rate constant, peak pump intensity, and pump pulse width, with an optimum pulse width required for maximal contrast.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
At typical values, the switch-off time is 1.6 microseconds and the switch-on time is 22.3 microseconds.
At typical values, the switch-off and switch-on time is 1.6 and 22.3 micros, respectively.
switch off time 1.6 microsecondsswitch on time 22.3 microseconds
The switching characteristics were used to design all-optical NOT, NOR, and NAND logic gates.
The switching characteristics have also been used to design all-optical NOT and the universal NOR and NAND logic gates.
Transmission of a 660 nm continuous-wave probe beam through the LOV2 sample is switched by a 442 nm pulsed pump beam.
The transmission of a cw probe laser beam at 660 nm corresponding to the peak absorption of the first excited L-state, through the LOV2 sample, is switched by a pulsed pump laser beam at 442 nm that corresponds to the maximum initial D state absorption.
probe wavelength 660 nmpump wavelength 442 nm
For a given pump pulse intensity, there is an optimum pump pulse width that maximizes switching contrast.
It is shown that for a given pump pulse intensity, there is an optimum pump pulsewidth for which the switching contrast is maximum.
Switching characteristics are sensitive to concentration, L-state rate constant, peak pump intensity, and pump pulse width.
The switching characteristics are sensitive to various parameters such as concentration, rate constant of L-state, peak pump intensity and pump pulse width.
The probe beam can be completely switched off with 100% modulation by the pump beam at 50 kW/cm2 for a 1 mM LOV2 sample with 5.5 mm thickness.
It is shown that the probe laser beam can be completely switched off (100% modulation) by the pump laser beam at 50 kW/cm2 for a concentration of 1 mM with sample thickness of 5.5 mm.
concentration 1 mMmodulation 100 %pump intensity 50 kW/cm2sample thickness 5.5 mm
The paper theoretically analyzes all-optical switching in the Avena sativa phot1 phototropin LOV2 domain based on nonlinear intensity-induced excited-state absorption.
We theoretically analyze all-optical switching in the recently characterized LOV2 domain from Avena sativa (oat) phot1 phototropin, a blue-light plant photoreceptor, based on nonlinear intensity-induced excited-state absorption.
Approval Evidence
1 source7 linked approval claimsfirst-pass slug lov2-domain-from-avena-sativa-phot1-phototropin
the recently characterized LOV2 domain from Avena sativa (oat) phot1 phototropin
Source:
kinetic performancesupports
At typical values, the switch-off time is 1.6 microseconds and the switch-on time is 22.3 microseconds.
At typical values, the switch-off and switch-on time is 1.6 and 22.3 micros, respectively.
Source:
logic gate designsupports
The switching characteristics were used to design all-optical NOT, NOR, and NAND logic gates.
The switching characteristics have also been used to design all-optical NOT and the universal NOR and NAND logic gates.
Source:
mechanism or operating principlesupports
Transmission of a 660 nm continuous-wave probe beam through the LOV2 sample is switched by a 442 nm pulsed pump beam.
The transmission of a cw probe laser beam at 660 nm corresponding to the peak absorption of the first excited L-state, through the LOV2 sample, is switched by a pulsed pump laser beam at 442 nm that corresponds to the maximum initial D state absorption.
Source:
optimization relationshipsupports
For a given pump pulse intensity, there is an optimum pump pulse width that maximizes switching contrast.
It is shown that for a given pump pulse intensity, there is an optimum pump pulsewidth for which the switching contrast is maximum.
Source:
parameter sensitivitysupports
Switching characteristics are sensitive to concentration, L-state rate constant, peak pump intensity, and pump pulse width.
The switching characteristics are sensitive to various parameters such as concentration, rate constant of L-state, peak pump intensity and pump pulse width.
Source:
performancesupports
The probe beam can be completely switched off with 100% modulation by the pump beam at 50 kW/cm2 for a 1 mM LOV2 sample with 5.5 mm thickness.
It is shown that the probe laser beam can be completely switched off (100% modulation) by the pump laser beam at 50 kW/cm2 for a concentration of 1 mM with sample thickness of 5.5 mm.
Source:
theoretical analysissupports
The paper theoretically analyzes all-optical switching in the Avena sativa phot1 phototropin LOV2 domain based on nonlinear intensity-induced excited-state absorption.
We theoretically analyze all-optical switching in the recently characterized LOV2 domain from Avena sativa (oat) phot1 phototropin, a blue-light plant photoreceptor, based on nonlinear intensity-induced excited-state absorption.
Source:
Comparisons
Source-backed strengths
The analysis reports complete probe suppression, with 100% modulation at 50 kW/cm2 in a 1 mM LOV2 sample of 5.5 mm thickness. Reported kinetics are microsecond-scale, with a switch-off time of 1.6 microseconds and a switch-on time of 22.3 microseconds, and the switching behavior was sufficient to support theoretical NOT, NOR, and NAND gate designs.
Source:
It is shown that for a given pump pulse intensity, there is an optimum pump pulsewidth for which the switching contrast is maximum.
Source:
It is shown that the probe laser beam can be completely switched off (100% modulation) by the pump laser beam at 50 kW/cm2 for a concentration of 1 mM with sample thickness of 5.5 mm.
Ranked Citations
- 1.