Source 1primary paper2024Physical Chemistry Chemical Physics
Toolkit/LOV2 domain from Avena sativa
LOV2 domain from Avena sativa
Also known as: AsLOV2, wild-type AsLOV2
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The LOV2 domain from Avena sativa (AsLOV2) is a blue-light receptor protein domain. In the cited 2024 study, cofactor exchange with 5-deazaFMN converted AsLOV2 into a photoswitch that forms a light-induced thermodynamically stable adduct and undergoes repeatable photo-induced dark-state recovery.
Usefulness & Problems
Why this is useful
This system is useful as a light-responsive protein module for optogenetic design because the authors propose the 5-deazaFMN-based AsLOV2 variant as an alternative to wild-type AsLOV2. Its reported robust, repeatable photocycle without significant loss supports applications requiring reversible optical control.
Source:
Based on the data presented we propose the system as an alternative to wild-type AsLOV2 for applications in optogenetics.
Problem solved
It addresses the need for an alternative AsLOV2-based photoswitch with altered photochemical behavior relative to wild-type AsLOV2. Specifically, the cited work shows that cofactor exchange can produce a light-induced thermodynamically stable adduct while retaining photo-induced recovery to the dark state.
Source:
Based on the data presented we propose the system as an alternative to wild-type AsLOV2 for applications in optogenetics.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Mechanisms
blue-light sensinglight-induced adduct formationphoto-induced dark-state recoveryphotoswitchingTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The reported variant requires cofactor exchange of AsLOV2 with 5-deazaFMN. The cited characterization used absorption spectroscopy, solution NMR spectroscopy, and isotopic labeling, but the provided evidence does not specify construct architecture, expression system, or delivery method.
The supplied evidence is limited to a single 2024 spectroscopy study and an application proposal for optogenetics. No direct in-cell, organismal, or independent validation data are provided here, and no quantitative performance metrics are supplied in the evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The authors propose the 5-deazaFMN-based AsLOV2 system as an alternative to wild-type AsLOV2 for optogenetics applications.
Based on the data presented we propose the system as an alternative to wild-type AsLOV2 for applications in optogenetics.
The authors propose the 5-deazaFMN-based AsLOV2 system as an alternative to wild-type AsLOV2 for optogenetics applications.
Based on the data presented we propose the system as an alternative to wild-type AsLOV2 for applications in optogenetics.
The authors propose the 5-deazaFMN-based AsLOV2 system as an alternative to wild-type AsLOV2 for optogenetics applications.
Based on the data presented we propose the system as an alternative to wild-type AsLOV2 for applications in optogenetics.
The authors propose the 5-deazaFMN-based AsLOV2 system as an alternative to wild-type AsLOV2 for optogenetics applications.
Based on the data presented we propose the system as an alternative to wild-type AsLOV2 for applications in optogenetics.
The authors propose the 5-deazaFMN-based AsLOV2 system as an alternative to wild-type AsLOV2 for optogenetics applications.
Based on the data presented we propose the system as an alternative to wild-type AsLOV2 for applications in optogenetics.
The authors propose the 5-deazaFMN-based AsLOV2 system as an alternative to wild-type AsLOV2 for optogenetics applications.
Based on the data presented we propose the system as an alternative to wild-type AsLOV2 for applications in optogenetics.
The authors propose the 5-deazaFMN-based AsLOV2 system as an alternative to wild-type AsLOV2 for optogenetics applications.
Based on the data presented we propose the system as an alternative to wild-type AsLOV2 for applications in optogenetics.
Cofactor exchange of AsLOV2 with 5-deazaFMN yields a light-induced thermodynamically stable adduct.
We report the photochemical changes of AsLOV2 through cofactor exchange with the FMN analogue 5-deazaFMN. Absorption spectroscopy shows that upon illumination a thermodynamically stable adduct is formed.
Cofactor exchange of AsLOV2 with 5-deazaFMN yields a light-induced thermodynamically stable adduct.
We report the photochemical changes of AsLOV2 through cofactor exchange with the FMN analogue 5-deazaFMN. Absorption spectroscopy shows that upon illumination a thermodynamically stable adduct is formed.
Cofactor exchange of AsLOV2 with 5-deazaFMN yields a light-induced thermodynamically stable adduct.
We report the photochemical changes of AsLOV2 through cofactor exchange with the FMN analogue 5-deazaFMN. Absorption spectroscopy shows that upon illumination a thermodynamically stable adduct is formed.
Cofactor exchange of AsLOV2 with 5-deazaFMN yields a light-induced thermodynamically stable adduct.
We report the photochemical changes of AsLOV2 through cofactor exchange with the FMN analogue 5-deazaFMN. Absorption spectroscopy shows that upon illumination a thermodynamically stable adduct is formed.
Cofactor exchange of AsLOV2 with 5-deazaFMN yields a light-induced thermodynamically stable adduct.
We report the photochemical changes of AsLOV2 through cofactor exchange with the FMN analogue 5-deazaFMN. Absorption spectroscopy shows that upon illumination a thermodynamically stable adduct is formed.
Cofactor exchange of AsLOV2 with 5-deazaFMN yields a light-induced thermodynamically stable adduct.
We report the photochemical changes of AsLOV2 through cofactor exchange with the FMN analogue 5-deazaFMN. Absorption spectroscopy shows that upon illumination a thermodynamically stable adduct is formed.
Cofactor exchange of AsLOV2 with 5-deazaFMN yields a light-induced thermodynamically stable adduct.
We report the photochemical changes of AsLOV2 through cofactor exchange with the FMN analogue 5-deazaFMN. Absorption spectroscopy shows that upon illumination a thermodynamically stable adduct is formed.
The 5-deazaFMN-based AsLOV2 system supports photo-induced dark-state recovery and functions as an easy-to-manipulate photoswitch with a repeatable robust photocycle without significant loss.
Dark-adapted state recovery can be photo-induced, providing a photoswitch that is easy to manipulate. The robust photocycle is repeatable without significant loss.
The 5-deazaFMN-based AsLOV2 system supports photo-induced dark-state recovery and functions as an easy-to-manipulate photoswitch with a repeatable robust photocycle without significant loss.
Dark-adapted state recovery can be photo-induced, providing a photoswitch that is easy to manipulate. The robust photocycle is repeatable without significant loss.
The 5-deazaFMN-based AsLOV2 system supports photo-induced dark-state recovery and functions as an easy-to-manipulate photoswitch with a repeatable robust photocycle without significant loss.
Dark-adapted state recovery can be photo-induced, providing a photoswitch that is easy to manipulate. The robust photocycle is repeatable without significant loss.
The 5-deazaFMN-based AsLOV2 system supports photo-induced dark-state recovery and functions as an easy-to-manipulate photoswitch with a repeatable robust photocycle without significant loss.
Dark-adapted state recovery can be photo-induced, providing a photoswitch that is easy to manipulate. The robust photocycle is repeatable without significant loss.
The 5-deazaFMN-based AsLOV2 system supports photo-induced dark-state recovery and functions as an easy-to-manipulate photoswitch with a repeatable robust photocycle without significant loss.
Dark-adapted state recovery can be photo-induced, providing a photoswitch that is easy to manipulate. The robust photocycle is repeatable without significant loss.
The 5-deazaFMN-based AsLOV2 system supports photo-induced dark-state recovery and functions as an easy-to-manipulate photoswitch with a repeatable robust photocycle without significant loss.
Dark-adapted state recovery can be photo-induced, providing a photoswitch that is easy to manipulate. The robust photocycle is repeatable without significant loss.
The 5-deazaFMN-based AsLOV2 system supports photo-induced dark-state recovery and functions as an easy-to-manipulate photoswitch with a repeatable robust photocycle without significant loss.
Dark-adapted state recovery can be photo-induced, providing a photoswitch that is easy to manipulate. The robust photocycle is repeatable without significant loss.
The structure of the light-induced adduct in the 5-deazaFMN-exchanged AsLOV2 system was confirmed by solution NMR using 13C-labelled 5-deazaFMN isotopologues.
We were able to confirm the structure of the adduct by introducing 13C-labelled 5-deazaFMN isotopologues in solution NMR experiments.
The structure of the light-induced adduct in the 5-deazaFMN-exchanged AsLOV2 system was confirmed by solution NMR using 13C-labelled 5-deazaFMN isotopologues.
We were able to confirm the structure of the adduct by introducing 13C-labelled 5-deazaFMN isotopologues in solution NMR experiments.
The structure of the light-induced adduct in the 5-deazaFMN-exchanged AsLOV2 system was confirmed by solution NMR using 13C-labelled 5-deazaFMN isotopologues.
We were able to confirm the structure of the adduct by introducing 13C-labelled 5-deazaFMN isotopologues in solution NMR experiments.
The structure of the light-induced adduct in the 5-deazaFMN-exchanged AsLOV2 system was confirmed by solution NMR using 13C-labelled 5-deazaFMN isotopologues.
We were able to confirm the structure of the adduct by introducing 13C-labelled 5-deazaFMN isotopologues in solution NMR experiments.
The structure of the light-induced adduct in the 5-deazaFMN-exchanged AsLOV2 system was confirmed by solution NMR using 13C-labelled 5-deazaFMN isotopologues.
We were able to confirm the structure of the adduct by introducing 13C-labelled 5-deazaFMN isotopologues in solution NMR experiments.
The structure of the light-induced adduct in the 5-deazaFMN-exchanged AsLOV2 system was confirmed by solution NMR using 13C-labelled 5-deazaFMN isotopologues.
We were able to confirm the structure of the adduct by introducing 13C-labelled 5-deazaFMN isotopologues in solution NMR experiments.
The structure of the light-induced adduct in the 5-deazaFMN-exchanged AsLOV2 system was confirmed by solution NMR using 13C-labelled 5-deazaFMN isotopologues.
We were able to confirm the structure of the adduct by introducing 13C-labelled 5-deazaFMN isotopologues in solution NMR experiments.
Approval Evidence
1 source1 linked approval claimfirst-pass slug lov2-domain-from-i-avena-sativa-i
The LOV2 domain from Avena sativa (As) is a blue light receptor
Source:
application proposalsupports
The authors propose the 5-deazaFMN-based AsLOV2 system as an alternative to wild-type AsLOV2 for optogenetics applications.
Based on the data presented we propose the system as an alternative to wild-type AsLOV2 for applications in optogenetics.
Source:
Comparisons
Source-backed strengths
The 2024 study reports that 5-deazaFMN-exchanged AsLOV2 forms a light-induced thermodynamically stable adduct. It also supports repeatable, robust photo-induced dark-state recovery without significant loss, indicating durable photoswitching behavior under repeated cycling.
Ranked Citations
- 1.