Source 1primary paper2009Biophysical Journal
Toolkit/LOV2/Jα domain from Avena sativa phototropin 1
LOV2/Jα domain from Avena sativa phototropin 1
Also known as: Avena sativa phototropin 1 LOV2/Jα domain, LOV2/Jα domain
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The LOV2/Jα domain from Avena sativa phototropin 1 is a light-responsive protein domain examined for conformational heterogeneity and propagation of structural changes. The supplied evidence specifically supports its structural characterization by temperature-dependent FTIR spectroscopy.
Usefulness & Problems
Why this is useful
This domain is useful as a model system for studying how light-responsive protein domains undergo and transmit structural changes. The provided evidence supports utility for mechanistic structural analysis rather than direct evidence of engineered functional output.
Problem solved
It helps address the problem of characterizing conformational heterogeneity and the propagation of light-induced structural changes within a photosensory protein domain. The supplied evidence does not establish a specific downstream application beyond this structural problem.
Problem links
Need precise spatiotemporal control with light input
DerivedThe LOV2/Jα domain from Avena sativa phototropin 1 is a light-responsive protein domain studied for conformational heterogeneity and propagation of structural changes. The supplied evidence supports its characterization by temperature-dependent FTIR spectroscopy, but does not provide direct functional performance details.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Techniques
Structural CharacterizationTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: actuatorswitch architecture: multi componentswitch architecture: uncaging
The evidence identifies the domain as originating from Avena sativa phototropin 1 and being studied by temperature-dependent FTIR spectroscopy. No further practical details on expression, cofactor handling, illumination conditions, or construct design are provided in the supplied material.
The supplied evidence does not report functional performance metrics, dynamic range, kinetics, reversibility, or use in engineered cellular systems. It also does not provide implementation details such as chromophore requirements, construct boundaries, or validation across assay contexts.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
Approval Evidence
1 source1 linked approval claimfirst-pass slug lov2-j-domain-from-avena-sativa-phototropin-1
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
Source:
structural characterizationsupports
The paper studies conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 using temperature-dependent FTIR spectroscopy.
Conformational Heterogeneity and Propagation of Structural Changes in the LOV2/Jα Domain from Avena sativa Phototropin 1 as Recorded by Temperature-Dependent FTIR Spectroscopy
Source:
Comparisons
Source-backed strengths
The available evidence directly supports that the LOV2/Jα domain was analyzed with temperature-dependent FTIR spectroscopy to resolve conformational heterogeneity and structural change propagation. This indicates suitability for detailed biophysical interrogation of light-responsive conformational behavior.
Compared with Avena sativa phototropin LOV2 domain
LOV2/Jα domain from Avena sativa phototropin 1 and Avena sativa phototropin LOV2 domain address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational uncaging, conformational_uncaging; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice.
Compared with Light-Oxygen-Voltage domain
LOV2/Jα domain from Avena sativa phototropin 1 and Light-Oxygen-Voltage domain address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational uncaging, conformational_uncaging; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice.
Compared with photoswitchable inhibitory peptides
LOV2/Jα domain from Avena sativa phototropin 1 and photoswitchable inhibitory peptides address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational uncaging, conformational_uncaging; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice.
Ranked Citations
- 1.