Source 1primary paper2023
Toolkit/LOVdeg tag
LOVdeg tag
Also known as: LOVdeg
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
LOVdeg is an engineered protein tag that is appended to a protein of interest to enable blue-light-inducible degradation in Escherichia coli. It provides optically controlled post-translational regulation by coupling light exposure to loss of the tagged protein.
Usefulness & Problems
Why this is useful
LOVdeg is useful for imposing light-controlled protein depletion in E. coli without changing the encoded target protein beyond tag fusion. The cited work also reports its use in a metabolic engineering application to demonstrate post-translational control of metabolism.
Source:
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
Source:
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
Source:
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
Problem solved
LOVdeg addresses the need for inducible, post-translational control of protein abundance in E. coli using a light input. The available evidence specifically supports blue-light-triggered degradation of tagged proteins rather than transcriptional regulation.
Source:
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
Source:
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
Problem links
Need conditional protein clearance
DerivedLOVdeg is an engineered protein tag that can be appended to a protein of interest to enable blue-light-inducible degradation in Escherichia coli. It provides optically controlled post-translational regulation by coupling light input to loss of the tagged protein.
Need controllable genome or transcript editing
DerivedLOVdeg is an engineered protein tag that can be appended to a protein of interest to enable blue-light-inducible degradation in Escherichia coli. It provides optically controlled post-translational regulation by coupling light input to loss of the tagged protein.
Need precise spatiotemporal control with light input
DerivedLOVdeg is an engineered protein tag that can be appended to a protein of interest to enable blue-light-inducible degradation in Escherichia coli. It provides optically controlled post-translational regulation by coupling light input to loss of the tagged protein.
Need tighter control over protein production
DerivedLOVdeg is an engineered protein tag that can be appended to a protein of interest to enable blue-light-inducible degradation in Escherichia coli. It provides optically controlled post-translational regulation by coupling light input to loss of the tagged protein.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Mechanisms
Degradationlight-inducible degradationlight-inducible degradationpost-translational controlpost-translational controlTranslation ControlTechniques
No technique tags yet.
Target processes
degradationeditingtranslationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: single chain
LOVdeg is implemented as a fusion tag appended to a protein of interest in E. coli. The available evidence specifies blue light as the inducing input, but does not report construct architecture, linker design, cofactor requirements, or delivery details.
The supplied evidence does not provide quantitative performance data, target scope, degradation kinetics, dynamic range, reversibility, or off-target effects. It also does not specify the molecular components responsible for degradation or validation outside E. coli.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2024
Ranked Claims
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
Approval Evidence
2 sources10 linked approval claimsfirst-pass slug lovdeg-tag
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
Source:
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
Source:
applicationsupports
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
Source:
engineering resultsupports
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
Source:
modularitysupports
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
Source:
overall conclusionsupports
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
Source:
performance enhancementsupports
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Source:
application demosupports
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
Source:
engineering resultsupports
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
Source:
modularitysupports
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
Source:
performance enhancementsupports
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Source:
tool introductionsupports
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
Source:
Comparisons
Source-backed strengths
The reported strength of LOVdeg is that it was engineered specifically as a blue-light-inducible degradation tag for proteins of interest in E. coli. It was also applied in a metabolic engineering context, supporting utility beyond a single proof-of-concept construct.
Source:
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
Source:
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Source:
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
Source:
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Compared with intein
LOVdeg tag and intein address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: post-translational control, translation_control; same primary input modality: light
Strengths here: appears more independently replicated; looks easier to implement in practice.
Compared with LOV2 domain-based optogenetic tool
LOVdeg tag and LOV2 domain-based optogenetic tool address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation; same primary input modality: light
Strengths here: appears more independently replicated; looks easier to implement in practice.
Compared with photosensitive degron
LOVdeg tag and photosensitive degron address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation; same primary input modality: light
Ranked Citations
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