Source 1review2021Chemical Society Reviews
Toolkit/luciferin-luciferase pair
luciferin-luciferase pair
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The different luciferin-luciferase pairs have different light emission wavelengths and hence are suitable for various applications.
Usefulness & Problems
Why this is useful
A luciferin-luciferase pair generates light when luciferin is oxidized by luciferase to form an excited-state emitter. The review frames these pairs as the core functional units behind many bioluminescence applications.; gene assays; detection of protein-protein interactions; high-throughput screening in drug discovery; in vivo imaging in small mammals
Source:
A luciferin-luciferase pair generates light when luciferin is oxidized by luciferase to form an excited-state emitter. The review frames these pairs as the core functional units behind many bioluminescence applications.
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gene assays
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detection of protein-protein interactions
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high-throughput screening in drug discovery
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in vivo imaging in small mammals
Problem solved
These pairs provide a genetically or chemically addressable light output for assays, interaction detection, screening, and imaging. Their wavelength diversity helps match the reporter to the intended use case.; provides light-generating reporter output for sensing and imaging applications; enables application-specific selection through differing emission wavelengths
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These pairs provide a genetically or chemically addressable light output for assays, interaction detection, screening, and imaging. Their wavelength diversity helps match the reporter to the intended use case.
Source:
provides light-generating reporter output for sensing and imaging applications
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enables application-specific selection through differing emission wavelengths
Problem links
enables application-specific selection through differing emission wavelengths
LiteratureThese pairs provide a genetically or chemically addressable light output for assays, interaction detection, screening, and imaging. Their wavelength diversity helps match the reporter to the intended use case.
Source:
These pairs provide a genetically or chemically addressable light output for assays, interaction detection, screening, and imaging. Their wavelength diversity helps match the reporter to the intended use case.
provides light-generating reporter output for sensing and imaging applications
LiteratureThese pairs provide a genetically or chemically addressable light output for assays, interaction detection, screening, and imaging. Their wavelength diversity helps match the reporter to the intended use case.
Source:
These pairs provide a genetically or chemically addressable light output for assays, interaction detection, screening, and imaging. Their wavelength diversity helps match the reporter to the intended use case.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
enzymatic oxidation of luciferin by luciferaseexcited-state light emission (bioluminescence)Target processes
recombinationselectionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
Implementation requires both the small-molecule luciferin and the corresponding luciferase enzyme. Choice of pair also depends on the emission wavelength needed for the application.; requires a luciferin substrate and a luciferase enzyme; application suitability depends on the emission wavelength of the chosen pair
The abstract does not indicate that any single pair is optimal for all applications. It also notes that many bioluminescent systems remain uncharacterised.; only 11 luciferin-luciferase systems are stated to be characterised to date
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Bioluminescence is routinely used for gene assays, protein-protein interaction detection, high-throughput screening in drug discovery, hygiene control, pollution analysis, and in vivo imaging in small mammals.
Different luciferin-luciferase pairs have different emission wavelengths, making them suitable for different applications.
Advances in protein engineering, synthetic chemistry, and physics have expanded luciferins and luciferases into previously uncharted applications over the last decade.
Bioluminescence occurs when luciferin is oxidized by luciferase to form an excited-state species that emits light.
More than 30 bioluminescent systems are known, but only 11 luciferin-luciferase pairs are stated to have been characterised to date.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug luciferin-luciferase-pair
The different luciferin-luciferase pairs have different light emission wavelengths and hence are suitable for various applications.
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application summarysupports
Bioluminescence is routinely used for gene assays, protein-protein interaction detection, high-throughput screening in drug discovery, hygiene control, pollution analysis, and in vivo imaging in small mammals.
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application summarysupports
Different luciferin-luciferase pairs have different emission wavelengths, making them suitable for different applications.
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field trendsupports
Advances in protein engineering, synthetic chemistry, and physics have expanded luciferins and luciferases into previously uncharted applications over the last decade.
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mechanism summarysupports
Bioluminescence occurs when luciferin is oxidized by luciferase to form an excited-state species that emits light.
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scope summarysupports
More than 30 bioluminescent systems are known, but only 11 luciferin-luciferase pairs are stated to have been characterised to date.
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Comparisons
Source-stated alternatives
The abstract contrasts different luciferin-luciferase pairs rather than naming a single universal system. It implies that alternative pairs are chosen based on wavelength and application fit.
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The abstract contrasts different luciferin-luciferase pairs rather than naming a single universal system. It implies that alternative pairs are chosen based on wavelength and application fit.
Source-backed strengths
different pairs provide different light emission wavelengths; supports a wide range of biotechnology and biomedical applications
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different pairs provide different light emission wavelengths
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supports a wide range of biotechnology and biomedical applications
Compared with CfRhPDE1
luciferin-luciferase pair and CfRhPDE1 address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: light
Compared with CheRiff
luciferin-luciferase pair and CheRiff address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: light
Compared with midbrain organoids
luciferin-luciferase pair and midbrain organoids address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: light
Ranked Citations
- 1.