Source 1primary paper2023ACS Central Science
Toolkit/LUNAS
LUNAS
Also known as: bioluminescent nucleic acid sensor, RPA-LUNAS, RT-RPA-LUNAS assay
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
LUNAS is a bioluminescent nucleic acid sensor in which a target double-stranded DNA sequence is recognized by two dCas9-based probes that mediate split NanoLuc luciferase complementation. Reported implementations couple this sensor to recombinase polymerase amplification, including RT-RPA-LUNAS for SARS-CoV-2 RNA detection.
Usefulness & Problems
Why this is useful
LUNAS provides sequence-specific nucleic acid detection with a bioluminescent output that can be monitored in real time. In reported one-pot RPA-coupled formats, it enables rapid, sensitive detection and a ratiometric readout that can be captured with a simple digital camera.
Source:
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Source:
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
Problem solved
This tool addresses the need for rapid nucleic acid diagnostics that combine sequence-specific recognition with simple optical readout under isothermal conditions. The reported system specifically supports detection of amplified target nucleic acids, including SARS-CoV-2 RNA after RT-RPA.
Source:
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
Problem links
LUNAS is explicitly a sequence-specific nucleic acid sensor for dsDNA, so it is mechanistically relevant to identifying infectious causes. It may help create more specific pathogen detection assays than nonspecific screening approaches.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
bioluminescent signal generationbioluminescent signal generationisothermal amplification via recombinase polymerase amplificationisothermal amplification via recombinase polymerase amplificationratiometric luminescence readoutratiometric luminescence readoutsequence-specific dcas9 binding to target dsdnasequence-specific dcas9 binding to target dsdnasplit luciferase complementationsplit luciferase complementationTechniques
Computational DesignComputational DesignComputational DesignFunctional AssayFunctional AssayFunctional AssayTarget processes
diagnosticrecombinationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenoperating role: sensorswitch architecture: multi componentswitch architecture: split
The core construct uses a pair of dCas9-based probes designed to bind a target dsDNA sequence and drive split NanoLuc complementation. Reported implementations integrate the sensor with recombinase polymerase amplification in a one-pot assay, and a calibrator luciferase is included for ratiometric luminescence readout and real-time monitoring.
The supplied evidence is limited to a single 2023 study and focuses on RPA-coupled diagnostic use cases, especially SARS-CoV-2. The provided evidence does not describe broader organismal validation, comparative benchmarking against other diagnostic platforms, or independent replication.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
time to detection ∼20 minviral RNA load ∼200 cp/μL
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
sensitivity attomolar
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
Approval Evidence
1 source6 linked approval claimsfirst-pass slug lunas
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Source:
diagnostic performancesupports
RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation and demonstrated its diagnostic performance for COVID-19 patient nasopharyngeal swab samples. Detection of SARS-CoV-2 from samples with viral RNA loads of ∼200 cp/μL was achieved within ∼20 min
Source:
integration capabilitysupports
LUNAS can be integrated with recombinase polymerase amplification in a rapid one-pot assay with attomolar sensitivity.
LUNAS is easily integrated with recombinase polymerase amplification (RPA), providing attomolar sensitivity in a rapid one-pot assay.
Source:
readout capabilitysupports
A calibrator luciferase enables robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
A calibrator luciferase is included for a robust ratiometric readout, enabling real-time monitoring of the RPA reaction using a simple digital camera.
Source:
sample processing capabilitysupports
The RT-RPA-LUNAS assay allows SARS-CoV-2 RNA detection without the need for RNA isolation.
We designed an RT-RPA-LUNAS assay that allows SARS-CoV-2 RNA detection without the need for cumbersome RNA isolation
Source:
tool descriptionsupports
LUNAS is a bioluminescent nucleic acid sensor platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Here, we developed a bioluminescent nucleic acid sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by a pair of dCas9-based probes mediating split NanoLuc luciferase complementation.
Source:
use case positioningsupports
RPA-LUNAS is attractive for point-of-care infectious disease testing.
showing that RPA-LUNAS is attractive for point-of-care infectious disease testing.
Source:
Comparisons
Source-backed strengths
The source reports that one-pot RPA-LUNAS achieved attomolar sensitivity and that RPA-LUNAS detected SARS-CoV-2 from patient nasopharyngeal swab samples with viral RNA loads of approximately 200 cp/μL within approximately 20 minutes. A calibrator luciferase enabled robust ratiometric readout and real-time monitoring of the RPA reaction using a simple digital camera.
Compared with ArrayG
LUNAS and ArrayG address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination
Strengths here: looks easier to implement in practice.
Compared with photoactivatable CRISPR/Cas12a system
LUNAS and photoactivatable CRISPR/Cas12a system address a similar problem space because they share diagnostic, recombination.
Shared frame: same top-level item type; shared target processes: diagnostic, recombination
Relative tradeoffs: appears more independently replicated.
Compared with SIBR-Cas
LUNAS and SIBR-Cas address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination
Ranked Citations
- 1.