Source 1primary paper2019Advanced Science
Toolkit/macrophage transient horizontal gene transfer
macrophage transient horizontal gene transfer
Also known as: macrophages as a drug carrier, macrophage transient horizontal gene transfer
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Macrophage transient horizontal gene transfer is a cell-based siRNA delivery harness in which macrophages loaded with siRNA lipoplexes transfer siRNA to cancer cells during in vitro coculture. In the reported system, transferred CIB1-siRNA reduced tumorsphere growth and lowered CIB1 and KI67 mRNA expression in MDA-MB-468 human breast cancer cells.
Usefulness & Problems
Why this is useful
This system is useful as a macrophage-mediated route for delivering siRNA cargo to cancer cells without direct transfection of the target cells. The reported study shows that transfer magnitude depends on siRNA loading and macrophage number, providing tunable control over intercellular delivery in coculture.
Source:
Here, it is demonstrated that macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Source:
Macrophages loaded with calcium integrin binding protein-1 (CIB1)-siRNA result in decreased tumorsphere growth and decreased mRNA expression of CIB1 and KI67 in MDA-MB-468 human breast cancer cells.
Problem solved
It addresses the problem of moving siRNA from a carrier cell population into cancer cells in vitro and achieving functional gene knockdown after transfer. The demonstrated application was delivery of CIB1-siRNA to MDA-MB-468 breast cancer cells, with associated reduction in tumorsphere growth and target-associated mRNA readouts.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.
Mechanisms
cell-mediated horizontal transfer of sirnarab27a-modulated intercellular cargo transferrna interference via transferred sirnaTechniques
No technique tags yet.
Target processes
recombinationImplementation Constraints
The reported implementation uses macrophages as carrier cells loaded with siRNA lipoplexes and then delivered into coculture with cancer cells. Practical performance depends on the amount of siRNA loaded into macrophages and the total number of macrophages used, and functional validation was reported in MDA-MB-468 tumorsphere and mRNA knockdown assays.
The evidence provided is limited to a single 2019 study and in vitro coculture experiments. The supplied evidence does not establish in vivo performance, generality across tumor types or siRNA cargos, or detailed transfer mechanism beyond macrophage-mediated intercellular cargo transfer.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Here, it is demonstrated that macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Here, it is demonstrated that macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Here, it is demonstrated that macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Here, it is demonstrated that macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Here, it is demonstrated that macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Here, it is demonstrated that macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Here, it is demonstrated that macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
The amount of siRNA transfer depends on the amount of siRNA loaded and the total number of macrophages delivered.
The amount of transfer can be dosed depending on the amount of siRNA loaded and total number of macrophages delivered.
The amount of siRNA transfer depends on the amount of siRNA loaded and the total number of macrophages delivered.
The amount of transfer can be dosed depending on the amount of siRNA loaded and total number of macrophages delivered.
The amount of siRNA transfer depends on the amount of siRNA loaded and the total number of macrophages delivered.
The amount of transfer can be dosed depending on the amount of siRNA loaded and total number of macrophages delivered.
The amount of siRNA transfer depends on the amount of siRNA loaded and the total number of macrophages delivered.
The amount of transfer can be dosed depending on the amount of siRNA loaded and total number of macrophages delivered.
The amount of siRNA transfer depends on the amount of siRNA loaded and the total number of macrophages delivered.
The amount of transfer can be dosed depending on the amount of siRNA loaded and total number of macrophages delivered.
The amount of siRNA transfer depends on the amount of siRNA loaded and the total number of macrophages delivered.
The amount of transfer can be dosed depending on the amount of siRNA loaded and total number of macrophages delivered.
The amount of siRNA transfer depends on the amount of siRNA loaded and the total number of macrophages delivered.
The amount of transfer can be dosed depending on the amount of siRNA loaded and total number of macrophages delivered.
Macrophages loaded with CIB1-siRNA decrease tumorsphere growth and decrease CIB1 and KI67 mRNA expression in MDA-MB-468 human breast cancer cells.
Macrophages loaded with calcium integrin binding protein-1 (CIB1)-siRNA result in decreased tumorsphere growth and decreased mRNA expression of CIB1 and KI67 in MDA-MB-468 human breast cancer cells.
Macrophages loaded with CIB1-siRNA decrease tumorsphere growth and decrease CIB1 and KI67 mRNA expression in MDA-MB-468 human breast cancer cells.
Macrophages loaded with calcium integrin binding protein-1 (CIB1)-siRNA result in decreased tumorsphere growth and decreased mRNA expression of CIB1 and KI67 in MDA-MB-468 human breast cancer cells.
Macrophages loaded with CIB1-siRNA decrease tumorsphere growth and decrease CIB1 and KI67 mRNA expression in MDA-MB-468 human breast cancer cells.
Macrophages loaded with calcium integrin binding protein-1 (CIB1)-siRNA result in decreased tumorsphere growth and decreased mRNA expression of CIB1 and KI67 in MDA-MB-468 human breast cancer cells.
Macrophages loaded with CIB1-siRNA decrease tumorsphere growth and decrease CIB1 and KI67 mRNA expression in MDA-MB-468 human breast cancer cells.
Macrophages loaded with calcium integrin binding protein-1 (CIB1)-siRNA result in decreased tumorsphere growth and decreased mRNA expression of CIB1 and KI67 in MDA-MB-468 human breast cancer cells.
Macrophages loaded with CIB1-siRNA decrease tumorsphere growth and decrease CIB1 and KI67 mRNA expression in MDA-MB-468 human breast cancer cells.
Macrophages loaded with calcium integrin binding protein-1 (CIB1)-siRNA result in decreased tumorsphere growth and decreased mRNA expression of CIB1 and KI67 in MDA-MB-468 human breast cancer cells.
Macrophages loaded with CIB1-siRNA decrease tumorsphere growth and decrease CIB1 and KI67 mRNA expression in MDA-MB-468 human breast cancer cells.
Macrophages loaded with calcium integrin binding protein-1 (CIB1)-siRNA result in decreased tumorsphere growth and decreased mRNA expression of CIB1 and KI67 in MDA-MB-468 human breast cancer cells.
Macrophages loaded with CIB1-siRNA decrease tumorsphere growth and decrease CIB1 and KI67 mRNA expression in MDA-MB-468 human breast cancer cells.
Macrophages loaded with calcium integrin binding protein-1 (CIB1)-siRNA result in decreased tumorsphere growth and decreased mRNA expression of CIB1 and KI67 in MDA-MB-468 human breast cancer cells.
Macrophage activation and Rab27a modulate the macrophage-mediated siRNA transfer process.
Furthermore, it is reported that macrophage activation can modulate this transfer process as well as intracellular trafficking protein Rab27a.
Macrophage activation and Rab27a modulate the macrophage-mediated siRNA transfer process.
Furthermore, it is reported that macrophage activation can modulate this transfer process as well as intracellular trafficking protein Rab27a.
Macrophage activation and Rab27a modulate the macrophage-mediated siRNA transfer process.
Furthermore, it is reported that macrophage activation can modulate this transfer process as well as intracellular trafficking protein Rab27a.
Macrophage activation and Rab27a modulate the macrophage-mediated siRNA transfer process.
Furthermore, it is reported that macrophage activation can modulate this transfer process as well as intracellular trafficking protein Rab27a.
Macrophage activation and Rab27a modulate the macrophage-mediated siRNA transfer process.
Furthermore, it is reported that macrophage activation can modulate this transfer process as well as intracellular trafficking protein Rab27a.
Macrophage activation and Rab27a modulate the macrophage-mediated siRNA transfer process.
Furthermore, it is reported that macrophage activation can modulate this transfer process as well as intracellular trafficking protein Rab27a.
Macrophage activation and Rab27a modulate the macrophage-mediated siRNA transfer process.
Furthermore, it is reported that macrophage activation can modulate this transfer process as well as intracellular trafficking protein Rab27a.
Adoptively transferred macrophages transfected with CIB1-siRNA localize to orthotopic MDA-MB-468 tumor.
Adoptive transfer of macrophages transfected with CIB1-siRNA localizes to the orthotopic MDA-MB-468 tumor.
Adoptively transferred macrophages transfected with CIB1-siRNA localize to orthotopic MDA-MB-468 tumor.
Adoptive transfer of macrophages transfected with CIB1-siRNA localizes to the orthotopic MDA-MB-468 tumor.
Adoptively transferred macrophages transfected with CIB1-siRNA localize to orthotopic MDA-MB-468 tumor.
Adoptive transfer of macrophages transfected with CIB1-siRNA localizes to the orthotopic MDA-MB-468 tumor.
Adoptively transferred macrophages transfected with CIB1-siRNA localize to orthotopic MDA-MB-468 tumor.
Adoptive transfer of macrophages transfected with CIB1-siRNA localizes to the orthotopic MDA-MB-468 tumor.
Adoptively transferred macrophages transfected with CIB1-siRNA localize to orthotopic MDA-MB-468 tumor.
Adoptive transfer of macrophages transfected with CIB1-siRNA localizes to the orthotopic MDA-MB-468 tumor.
Adoptively transferred macrophages transfected with CIB1-siRNA localize to orthotopic MDA-MB-468 tumor.
Adoptive transfer of macrophages transfected with CIB1-siRNA localizes to the orthotopic MDA-MB-468 tumor.
Adoptively transferred macrophages transfected with CIB1-siRNA localize to orthotopic MDA-MB-468 tumor.
Adoptive transfer of macrophages transfected with CIB1-siRNA localizes to the orthotopic MDA-MB-468 tumor.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug macrophage-transient-horizontal-gene-transfer
Targeted Delivery of siRNA Lipoplexes to Cancer Cells Using Macrophage Transient Horizontal Gene Transfer
Source:
capabilitysupports
Macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Here, it is demonstrated that macrophages can horizontally transfer siRNA to cancer cells during in vitro coculture.
Source:
dose dependencesupports
The amount of siRNA transfer depends on the amount of siRNA loaded and the total number of macrophages delivered.
The amount of transfer can be dosed depending on the amount of siRNA loaded and total number of macrophages delivered.
Source:
functional effectsupports
Macrophages loaded with CIB1-siRNA decrease tumorsphere growth and decrease CIB1 and KI67 mRNA expression in MDA-MB-468 human breast cancer cells.
Macrophages loaded with calcium integrin binding protein-1 (CIB1)-siRNA result in decreased tumorsphere growth and decreased mRNA expression of CIB1 and KI67 in MDA-MB-468 human breast cancer cells.
Source:
modulationsupports
Macrophage activation and Rab27a modulate the macrophage-mediated siRNA transfer process.
Furthermore, it is reported that macrophage activation can modulate this transfer process as well as intracellular trafficking protein Rab27a.
Source:
tumor localizationsupports
Adoptively transferred macrophages transfected with CIB1-siRNA localize to orthotopic MDA-MB-468 tumor.
Adoptive transfer of macrophages transfected with CIB1-siRNA localizes to the orthotopic MDA-MB-468 tumor.
Source:
Comparisons
Source-backed strengths
The key strength is demonstrated horizontal transfer of siRNA from macrophages to cancer cells during coculture with measurable dose dependence on siRNA load and macrophage input. Functional activity was supported by decreased tumorsphere growth and reduced CIB1 and KI67 mRNA expression in MDA-MB-468 cells after macrophage loading with CIB1-siRNA.
Ranked Citations
- 1.