Source 1primary paper2025MED
Toolkit/MagMboI-plus
MagMboI-plus
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
MagMboI-plus is a redesigned photoactivatable endonuclease variant used as a light-controlled multi-component switch in Saccharomyces cerevisiae. In vivo, blue-light activation yields slightly increased DNA-cleavage activity relative to the original MagMboI construct, but also more pronounced genomic rearrangements.
Usefulness & Problems
Why this is useful
This tool is useful for light-gated induction of DNA cleavage in yeast, enabling temporal control over genome engineering workflows linked to recombination. The available evidence indicates improved activity over the original MagMboI construct, while also highlighting a tradeoff in genome stability.
Source:
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
Problem solved
MagMboI-plus addresses the need for externally controllable endonuclease activity during top-down genome engineering in Saccharomyces cerevisiae. Specifically, it provides a photoactivatable route to trigger cleavage-associated recombination processes with light rather than constitutive nuclease action.
Problem links
Need conditional recombination or state switching
DerivedMagMboI-plus is a redesigned photoactivatable endonuclease variant used as a light-controlled multi-component switch in Saccharomyces cerevisiae. Upon blue light activation, it shows slightly increased in vivo DNA-cleavage activity relative to the original MagMboI construct, but it also produces more pronounced genomic rearrangements.
Need precise spatiotemporal control with light input
DerivedMagMboI-plus is a redesigned photoactivatable endonuclease variant used as a light-controlled multi-component switch in Saccharomyces cerevisiae. Upon blue light activation, it shows slightly increased in vivo DNA-cleavage activity relative to the original MagMboI construct, but it also produces more pronounced genomic rearrangements.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Target processes
recombinationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedengineered variant: Trueimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementin vivo tested in yeast: Trueoperating role: actuatoroperating role: regulatorphotoactivatable: Trueswitch architecture: cleavageswitch architecture: multi componentswitch architecture: recruitment
The available evidence supports use in Saccharomyces cerevisiae and activation by blue light. No further practical details are provided here regarding cofactors, expression strategy, domain composition, or delivery format.
The same source reports that MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells. The supplied evidence does not provide quantitative performance metrics, detailed construct architecture, or validation outside yeast.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
mixedYeastapplication demoSaccharomyces cerevisiae
Inferred from claim c6 during normalization. In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct. Derived from claim c6.
Source:
DNA-cleavage activity change(slightly increased)
mixedYeastapplication demoSaccharomyces cerevisiae
Inferred from claim c6 during normalization. In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct. Derived from claim c6.
Source:
DNA-cleavage activity change(slightly increased)
mixedYeastapplication demoSaccharomyces cerevisiae
Inferred from claim c6 during normalization. In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct. Derived from claim c6.
Source:
DNA-cleavage activity change(slightly increased)
mixedYeastapplication demoSaccharomyces cerevisiae
Inferred from claim c6 during normalization. In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct. Derived from claim c6.
Source:
DNA-cleavage activity change(slightly increased)
mixedYeastapplication demoSaccharomyces cerevisiae
Inferred from claim c6 during normalization. In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct. Derived from claim c6.
Source:
DNA-cleavage activity change(slightly increased)
mixedYeastapplication demoSaccharomyces cerevisiae
Inferred from claim c6 during normalization. In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct. Derived from claim c6.
Source:
DNA-cleavage activity change(slightly increased)
mixedYeastapplication demoSaccharomyces cerevisiae
Inferred from claim c6 during normalization. In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct. Derived from claim c6.
Source:
DNA-cleavage activity change(slightly increased)
mixedYeastapplication demoSaccharomyces cerevisiae
Inferred from claim c6 during normalization. In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct. Derived from claim c6.
Source:
DNA-cleavage activity change(slightly increased)
Supporting Sources
Ranked Claims
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug magmboi-plus
This redesigned variant (designated MagMboI-plus) preserves b1-helical integrity while strengthening protein-DNA contacts. Although MagMboI-plus, when introduced in Saccharomyces cerevisiae cells, exhibited slightly increased DNA-cleavage activity in vivo upon blue light activation, it was found to induce more pronounced genomic rearrangements compared to the original MagMboI construct.
Source:
comparative liabilitysupports
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
Source:
comparative performancesupports
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
Source:
general strategysupports
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
Source:
variant improvementsupports
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
Source:
variant propertysupports
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
Source:
Comparisons
Source-backed strengths
The reported study describes MagMboI-plus as an optimized variant with slightly increased in vivo DNA-cleavage activity compared with the original MagMboI construct. It was evaluated in Saccharomyces cerevisiae in the context of top-down genome engineering, providing direct organism-level evidence for function.
Source:
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
Source:
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
Compared with GFP-PHR-caspase8/Flag-CIB1N-caspase8
MagMboI-plus and GFP-PHR-caspase8/Flag-CIB1N-caspase8 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: photocleavage; same primary input modality: light
Compared with PA-Cre 3.0
MagMboI-plus and PA-Cre 3.0 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: photocleavage; same primary input modality: light
Compared with photocaged IPTG
MagMboI-plus and photocaged IPTG address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: photocleavage; same primary input modality: light
Ranked Citations
- 1.