Source 1primary paper2012PLoS ONE
Toolkit/mammalian rod opsin
mammalian rod opsin
Also known as: rod opsin
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Mammalian rod opsin is an opsin-based light-responsive GPCR domain used in optogenetic contexts. In the cited evidence, it is discussed primarily as a comparator whose responses dissipate during repeated light exposure, consistent with bleaching.
Usefulness & Problems
Why this is useful
The cited evidence indicates that mammalian rod opsin provides a benchmark for evaluating optogenetic GPCR performance under repeated illumination. Its main utility in this record is as a reference point for limitations that alternative opsins were designed to overcome.
Source:
Visible light induced high amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp
Problem solved
Mammalian rod opsin addresses the general need for a light-activated GPCR module in optogenetic systems. However, the supplied evidence does not document a specific application success for this tool beyond its use as a comparator in repeated-exposure experiments.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The supplied evidence identifies mammalian rod opsin as a protein domain used in optogenetic contexts and mentions variants of mammalian rod opsin. No direct implementation details are provided here regarding chromophore requirements, construct architecture, expression system, or delivery method.
The key limitation supported by the cited study is dissipation of light responses during repeated exposure, attributed to rod opsin bleaching. No additional evidence is provided here on spectral properties, recovery behavior, coupling profile, or performance across cell types.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Replacing rod opsin with JellyOp overcomes the repeated-exposure dissipation limitation attributed to rod opsin bleaching.
replacing rod opsin with a bleach resistant opsin from Carybdea rastonii, the box jellyfish, (JellyOp) overcomes this limitation
Replacing rod opsin with JellyOp overcomes the repeated-exposure dissipation limitation attributed to rod opsin bleaching.
replacing rod opsin with a bleach resistant opsin from Carybdea rastonii, the box jellyfish, (JellyOp) overcomes this limitation
Replacing rod opsin with JellyOp overcomes the repeated-exposure dissipation limitation attributed to rod opsin bleaching.
replacing rod opsin with a bleach resistant opsin from Carybdea rastonii, the box jellyfish, (JellyOp) overcomes this limitation
Replacing rod opsin with JellyOp overcomes the repeated-exposure dissipation limitation attributed to rod opsin bleaching.
replacing rod opsin with a bleach resistant opsin from Carybdea rastonii, the box jellyfish, (JellyOp) overcomes this limitation
Replacing rod opsin with JellyOp overcomes the repeated-exposure dissipation limitation attributed to rod opsin bleaching.
replacing rod opsin with a bleach resistant opsin from Carybdea rastonii, the box jellyfish, (JellyOp) overcomes this limitation
Replacing rod opsin with JellyOp overcomes the repeated-exposure dissipation limitation attributed to rod opsin bleaching.
replacing rod opsin with a bleach resistant opsin from Carybdea rastonii, the box jellyfish, (JellyOp) overcomes this limitation
Replacing rod opsin with JellyOp overcomes the repeated-exposure dissipation limitation attributed to rod opsin bleaching.
replacing rod opsin with a bleach resistant opsin from Carybdea rastonii, the box jellyfish, (JellyOp) overcomes this limitation
Visible light induces high-amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp.
Visible light induced high amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp
Visible light induces high-amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp.
Visible light induced high amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp
Visible light induces high-amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp.
Visible light induced high amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp
Visible light induces high-amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp.
Visible light induced high amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp
Visible light induces high-amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp.
Visible light induced high amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp
Visible light induces high-amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp.
Visible light induced high amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp
Visible light induces high-amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp.
Visible light induced high amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp
JellyOp is presented as a promising optogenetic tool for mimicking the activity of Gs-coupled G protein-coupled receptors with fine spatiotemporal resolution.
We conclude that JellyOp is a promising new tool for mimicking the activity of Gs-coupled G protein coupled receptors with fine spatiotemporal resolution
JellyOp is presented as a promising optogenetic tool for mimicking the activity of Gs-coupled G protein-coupled receptors with fine spatiotemporal resolution.
We conclude that JellyOp is a promising new tool for mimicking the activity of Gs-coupled G protein coupled receptors with fine spatiotemporal resolution
JellyOp is presented as a promising optogenetic tool for mimicking the activity of Gs-coupled G protein-coupled receptors with fine spatiotemporal resolution.
We conclude that JellyOp is a promising new tool for mimicking the activity of Gs-coupled G protein coupled receptors with fine spatiotemporal resolution
JellyOp is presented as a promising optogenetic tool for mimicking the activity of Gs-coupled G protein-coupled receptors with fine spatiotemporal resolution.
We conclude that JellyOp is a promising new tool for mimicking the activity of Gs-coupled G protein coupled receptors with fine spatiotemporal resolution
JellyOp is presented as a promising optogenetic tool for mimicking the activity of Gs-coupled G protein-coupled receptors with fine spatiotemporal resolution.
We conclude that JellyOp is a promising new tool for mimicking the activity of Gs-coupled G protein coupled receptors with fine spatiotemporal resolution
JellyOp is presented as a promising optogenetic tool for mimicking the activity of Gs-coupled G protein-coupled receptors with fine spatiotemporal resolution.
We conclude that JellyOp is a promising new tool for mimicking the activity of Gs-coupled G protein coupled receptors with fine spatiotemporal resolution
JellyOp is presented as a promising optogenetic tool for mimicking the activity of Gs-coupled G protein-coupled receptors with fine spatiotemporal resolution.
We conclude that JellyOp is a promising new tool for mimicking the activity of Gs-coupled G protein coupled receptors with fine spatiotemporal resolution
Rod opsin-based optogenetic tools show light responses that dissipate under repeated exposure, consistent with bleaching.
the light response driven by such rod opsin-based tools dissipates under repeated exposure, consistent with the known bleaching characteristics of this photopigment
Rod opsin-based optogenetic tools show light responses that dissipate under repeated exposure, consistent with bleaching.
the light response driven by such rod opsin-based tools dissipates under repeated exposure, consistent with the known bleaching characteristics of this photopigment
Rod opsin-based optogenetic tools show light responses that dissipate under repeated exposure, consistent with bleaching.
the light response driven by such rod opsin-based tools dissipates under repeated exposure, consistent with the known bleaching characteristics of this photopigment
Rod opsin-based optogenetic tools show light responses that dissipate under repeated exposure, consistent with bleaching.
the light response driven by such rod opsin-based tools dissipates under repeated exposure, consistent with the known bleaching characteristics of this photopigment
Rod opsin-based optogenetic tools show light responses that dissipate under repeated exposure, consistent with bleaching.
the light response driven by such rod opsin-based tools dissipates under repeated exposure, consistent with the known bleaching characteristics of this photopigment
Rod opsin-based optogenetic tools show light responses that dissipate under repeated exposure, consistent with bleaching.
the light response driven by such rod opsin-based tools dissipates under repeated exposure, consistent with the known bleaching characteristics of this photopigment
Rod opsin-based optogenetic tools show light responses that dissipate under repeated exposure, consistent with bleaching.
the light response driven by such rod opsin-based tools dissipates under repeated exposure, consistent with the known bleaching characteristics of this photopigment
JellyOp is more photosensitive than currently available optogenetic tools and responds to white light at irradiances of at least 1 µW/cm(2).
JellyOp was more photosensitive than currently available optogenetic tools, responding to white light at irradiances ≥1 µW/cm(2)
white light irradiance response threshold 1 µW/cm(2)
JellyOp is more photosensitive than currently available optogenetic tools and responds to white light at irradiances of at least 1 µW/cm(2).
JellyOp was more photosensitive than currently available optogenetic tools, responding to white light at irradiances ≥1 µW/cm(2)
white light irradiance response threshold 1 µW/cm(2)
JellyOp is more photosensitive than currently available optogenetic tools and responds to white light at irradiances of at least 1 µW/cm(2).
JellyOp was more photosensitive than currently available optogenetic tools, responding to white light at irradiances ≥1 µW/cm(2)
white light irradiance response threshold 1 µW/cm(2)
JellyOp is more photosensitive than currently available optogenetic tools and responds to white light at irradiances of at least 1 µW/cm(2).
JellyOp was more photosensitive than currently available optogenetic tools, responding to white light at irradiances ≥1 µW/cm(2)
white light irradiance response threshold 1 µW/cm(2)
JellyOp is more photosensitive than currently available optogenetic tools and responds to white light at irradiances of at least 1 µW/cm(2).
JellyOp was more photosensitive than currently available optogenetic tools, responding to white light at irradiances ≥1 µW/cm(2)
white light irradiance response threshold 1 µW/cm(2)
JellyOp is more photosensitive than currently available optogenetic tools and responds to white light at irradiances of at least 1 µW/cm(2).
JellyOp was more photosensitive than currently available optogenetic tools, responding to white light at irradiances ≥1 µW/cm(2)
white light irradiance response threshold 1 µW/cm(2)
JellyOp is more photosensitive than currently available optogenetic tools and responds to white light at irradiances of at least 1 µW/cm(2).
JellyOp was more photosensitive than currently available optogenetic tools, responding to white light at irradiances ≥1 µW/cm(2)
white light irradiance response threshold 1 µW/cm(2)
Single flashes produce a brief cAMP spike, whereas repeated stimulation can sustain elevated cAMP levels for tens of minutes in JellyOp-expressing mammalian cells.
While single flashes produced a brief cAMP spike, repeated stimulation could sustain elevated levels for 10s of minutes
duration of sustained elevated cAMP 10s of minutes
Single flashes produce a brief cAMP spike, whereas repeated stimulation can sustain elevated cAMP levels for tens of minutes in JellyOp-expressing mammalian cells.
While single flashes produced a brief cAMP spike, repeated stimulation could sustain elevated levels for 10s of minutes
duration of sustained elevated cAMP 10s of minutes
Single flashes produce a brief cAMP spike, whereas repeated stimulation can sustain elevated cAMP levels for tens of minutes in JellyOp-expressing mammalian cells.
While single flashes produced a brief cAMP spike, repeated stimulation could sustain elevated levels for 10s of minutes
duration of sustained elevated cAMP 10s of minutes
Single flashes produce a brief cAMP spike, whereas repeated stimulation can sustain elevated cAMP levels for tens of minutes in JellyOp-expressing mammalian cells.
While single flashes produced a brief cAMP spike, repeated stimulation could sustain elevated levels for 10s of minutes
duration of sustained elevated cAMP 10s of minutes
Single flashes produce a brief cAMP spike, whereas repeated stimulation can sustain elevated cAMP levels for tens of minutes in JellyOp-expressing mammalian cells.
While single flashes produced a brief cAMP spike, repeated stimulation could sustain elevated levels for 10s of minutes
duration of sustained elevated cAMP 10s of minutes
Single flashes produce a brief cAMP spike, whereas repeated stimulation can sustain elevated cAMP levels for tens of minutes in JellyOp-expressing mammalian cells.
While single flashes produced a brief cAMP spike, repeated stimulation could sustain elevated levels for 10s of minutes
duration of sustained elevated cAMP 10s of minutes
Single flashes produce a brief cAMP spike, whereas repeated stimulation can sustain elevated cAMP levels for tens of minutes in JellyOp-expressing mammalian cells.
While single flashes produced a brief cAMP spike, repeated stimulation could sustain elevated levels for 10s of minutes
duration of sustained elevated cAMP 10s of minutes
Approval Evidence
1 source2 linked approval claimsfirst-pass slug mammalian-rod-opsin
variants of mammalian rod opsin
Source:
comparative advantagesupports
Replacing rod opsin with JellyOp overcomes the repeated-exposure dissipation limitation attributed to rod opsin bleaching.
replacing rod opsin with a bleach resistant opsin from Carybdea rastonii, the box jellyfish, (JellyOp) overcomes this limitation
Source:
limitationsupports
Rod opsin-based optogenetic tools show light responses that dissipate under repeated exposure, consistent with bleaching.
the light response driven by such rod opsin-based tools dissipates under repeated exposure, consistent with the known bleaching characteristics of this photopigment
Source:
Comparisons
Source-backed strengths
The evidence supports that mammalian rod opsin is sufficiently established to serve as a comparator against another metazoan opsin in mammalian-cell optogenetic studies. Beyond its identity as a light-responsive opsin module, the supplied record does not provide direct performance data for amplitude, reversibility, kinetics, or signaling specificity.
Source:
replacing rod opsin with a bleach resistant opsin from Carybdea rastonii, the box jellyfish, (JellyOp) overcomes this limitation
Ranked Citations
- 1.