Source 1review2016Cold Spring Harbor Perspectives in Biology
Toolkit/microencapsulated mammalian cells
microencapsulated mammalian cells
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Microencapsulated mammalian cells are a delivery harness in which engineered mammalian cells carrying closed-loop gene networks are implanted into mice. The available evidence supports this platform as a means to deploy synthetic mammalian gene circuits in vivo.
Usefulness & Problems
Why this is useful
This harness is useful for placing synthetic mammalian gene circuits into a living animal context rather than restricting them to cell culture. The cited review also states that mammalian gene circuits built from synthetic gene switches can support memory, oscillatory protein production, and complex information processing, indicating the kinds of functions that such implanted cells may deliver.
Source:
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Source:
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Problem solved
It addresses the problem of how to introduce engineered mammalian cells with synthetic closed-loop gene regulation into mice for in vivo operation. The evidence does not provide more specific therapeutic or experimental use cases beyond implantation of engineered cells.
Source:
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
Implementation requires mammalian cells engineered with closed-loop gene networks and a microencapsulation step before implantation into mice. The supplied evidence does not specify construct architecture, encapsulation chemistry, expression system, or any cofactor and delivery requirements.
The evidence is limited to a brief statement that microencapsulated engineered mammalian cells can be implanted into mice, without quantitative performance data. No details are provided on encapsulation materials, implantation site, cell type, durability, host response, or functional outcomes after implantation.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Approval Evidence
1 source1 linked approval claimfirst-pass slug microencapsulated-mammalian-cells
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice
Source:
therapeutic applicationsupports
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Source:
Comparisons
Source-backed strengths
A key strength is that the platform is explicitly described as compatible with mammalian cells engineered with closed-loop gene networks and implanted into mice. The supporting review further establishes that synthetic gene switches can serve as building blocks for complex mammalian circuits capable of memory, oscillation, and information-processing behaviors.
Ranked Citations
- 1.