Source 1primary paper2018Fungal Biology and Biotechnology
Toolkit/microhomology templates
microhomology templates
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Microhomology templates are donor DNA constructs used together with in vitro assembled Cas9-ribonucleoprotein complexes for CRISPR/Cas9-mediated gene targeting in Aspergillus fumigatus. In the cited study context, this combination supported reliable and highly efficient genome editing.
Usefulness & Problems
Why this is useful
These templates are useful as donor constructs for Cas9 RNP-based genome editing in Aspergillus fumigatus, where they support efficient and reliable gene targeting. The available evidence specifically supports their use in a CRISPR/Cas9 workflow coupled to in vitro assembled ribonucleoprotein delivery.
Problem solved
Microhomology templates address the problem of achieving reliable donor-directed gene targeting after Cas9-mediated genome cleavage in Aspergillus fumigatus. The cited evidence indicates that coupling them to in vitro assembled Cas9 RNPs improves the efficiency and reliability of CRISPR/Cas9 editing in this organism.
Problem links
Need controllable genome or transcript editing
DerivedMicrohomology templates are donor DNA constructs used together with in vitro assembled Cas9-ribonucleoprotein complexes for CRISPR/Cas9-mediated gene targeting in Aspergillus fumigatus. In the cited study context, this combination supported reliable and highly efficient genome editing.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
editingImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: regulator
The available evidence indicates use with in vitro assembled Cas9-ribonucleoprotein complexes and donor DNA microhomology templates in Aspergillus fumigatus. No further practical details are provided here regarding template architecture, delivery conditions, selectable markers, or repair junction design.
The supplied evidence is limited to Aspergillus fumigatus and does not provide sequence design rules, homology arm lengths, editing frequencies, or locus-specific performance. No independent replication, cross-species validation, or direct comparison to alternative donor formats is provided in the supplied material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The in vitro assembled Cas9-ribonucleoprotein and microhomology template system is highly efficient.
This system is highly efficient
The in vitro assembled Cas9-ribonucleoprotein and microhomology template system is highly efficient.
This system is highly efficient
The in vitro assembled Cas9-ribonucleoprotein and microhomology template system is highly efficient.
This system is highly efficient
The in vitro assembled Cas9-ribonucleoprotein and microhomology template system is highly efficient.
This system is highly efficient
The in vitro assembled Cas9-ribonucleoprotein and microhomology template system is highly efficient.
This system is highly efficient
The in vitro assembled Cas9-ribonucleoprotein and microhomology template system is highly efficient.
This system is highly efficient
CRISPR/Cas9-based genome editing in Aspergillus fumigatus using in vitro assembled Cas9-ribonucleoprotein complexes coupled with microhomology templates is a reliable method of gene targeting.
CRISPR/Cas9-based genome editing in A. fumigatus using in vitro assembled RNPs coupled with microhomology templates is a reliable method of gene targeting.
CRISPR/Cas9-based genome editing in Aspergillus fumigatus using in vitro assembled Cas9-ribonucleoprotein complexes coupled with microhomology templates is a reliable method of gene targeting.
CRISPR/Cas9-based genome editing in A. fumigatus using in vitro assembled RNPs coupled with microhomology templates is a reliable method of gene targeting.
CRISPR/Cas9-based genome editing in Aspergillus fumigatus using in vitro assembled Cas9-ribonucleoprotein complexes coupled with microhomology templates is a reliable method of gene targeting.
CRISPR/Cas9-based genome editing in A. fumigatus using in vitro assembled RNPs coupled with microhomology templates is a reliable method of gene targeting.
CRISPR/Cas9-based genome editing in Aspergillus fumigatus using in vitro assembled Cas9-ribonucleoprotein complexes coupled with microhomology templates is a reliable method of gene targeting.
CRISPR/Cas9-based genome editing in A. fumigatus using in vitro assembled RNPs coupled with microhomology templates is a reliable method of gene targeting.
CRISPR/Cas9-based genome editing in Aspergillus fumigatus using in vitro assembled Cas9-ribonucleoprotein complexes coupled with microhomology templates is a reliable method of gene targeting.
CRISPR/Cas9-based genome editing in A. fumigatus using in vitro assembled RNPs coupled with microhomology templates is a reliable method of gene targeting.
CRISPR/Cas9-based genome editing in Aspergillus fumigatus using in vitro assembled Cas9-ribonucleoprotein complexes coupled with microhomology templates is a reliable method of gene targeting.
CRISPR/Cas9-based genome editing in A. fumigatus using in vitro assembled RNPs coupled with microhomology templates is a reliable method of gene targeting.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug microhomology-templates
coupled with microhomology templates
Source:
efficiencysupports
The in vitro assembled Cas9-ribonucleoprotein and microhomology template system is highly efficient.
This system is highly efficient
Source:
reliabilitysupports
CRISPR/Cas9-based genome editing in Aspergillus fumigatus using in vitro assembled Cas9-ribonucleoprotein complexes coupled with microhomology templates is a reliable method of gene targeting.
CRISPR/Cas9-based genome editing in A. fumigatus using in vitro assembled RNPs coupled with microhomology templates is a reliable method of gene targeting.
Source:
Comparisons
Source-backed strengths
The reported system was described as highly efficient for gene targeting when microhomology templates were coupled with in vitro assembled Cas9-ribonucleoprotein complexes. It was also described as a reliable method for CRISPR/Cas9-based genome editing in Aspergillus fumigatus.
Compared with microfluidic organ-on-chip platforms
microhomology templates and microfluidic organ-on-chip platforms address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Strengths here: looks easier to implement in practice.
Compared with synthetic promoters
microhomology templates and synthetic promoters address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with Z7-E78-ABE
microhomology templates and Z7-E78-ABE address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Ranked Citations
- 1.