Source 1primary paper2025MED
Toolkit/mitochondrial cytochrome c-tagged Dendra2 reporter
mitochondrial cytochrome c-tagged Dendra2 reporter
Also known as: Dendra2, dendra2 protein, tagged to mitochondrial cytochrome c
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The mitochondrial cytochrome c-tagged Dendra2 reporter is a photoswitchable fluorescent protein construct used to monitor mitochondrial fission and fusion dynamics. In the cited method, Dendra2 is fused to mitochondrial cytochrome c and imaged by confocal microscopy with Fiji-based image analysis.
Usefulness & Problems
Why this is useful
This reporter is useful for visualizing and quantifying mitochondrial dynamics in live-cell imaging workflows. The cited method specifically uses it with confocal microscopy and free-to-use ImageJ (Fiji) tools to assess fission/fusion events.
Problem solved
It addresses the need for an optical reporter that enables analysis of mitochondrial fission and fusion behavior. The available evidence supports its use as a photoswitchable mitochondrial marker in an image-analysis pipeline for these events.
Published Workflows
Objective: Assess mitochondrial fission/fusion dynamics in kidney proximal tubular cells using confocal microscopy, Fiji/ImageJ analysis, and a photoswitchable mitochondrial Dendra2 reporter.
Why it works: The abstract states that the assay studies fission/fusion events using the photo-switching property of Dendra2 tagged to mitochondrial cytochrome c, combined with confocal microscopy and Fiji/ImageJ analysis.
photo-switching of a mitochondrial Dendra2 reporter to monitor mitochondrial fission/fusion eventsconfocal microscopyImageJ (Fiji)-based quantitative analysis
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Mechanisms
photoswitchingTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The construct consists of Dendra2 tagged to mitochondrial cytochrome c, indicating a domain-fusion design for mitochondrial labeling. The reported workflow uses confocal microscopy and ImageJ (Fiji) image processing; no additional construct architecture, expression system, or cofactor requirements are provided in the supplied evidence.
The supplied evidence is limited to a method description and does not report quantitative benchmarking, photophysical parameters, or comparisons to alternative mitochondrial reporters. Validation is only described in the context of assessing mitochondrial dynamics in kidney proximal tubular cells.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2review2014Annual Review of Biophysics
Ranked Claims
The article describes using confocal microscopy together with free-to-use ImageJ (Fiji) tools to study mitochondrial fission/fusion events using a photoswitchable Dendra2 reporter tagged to mitochondrial cytochrome c.
In this article, we describe the use of confocal microscopy combined with free-to-use tools in ImageJ (Fiji) to study fission/fusion events using the photo-switching property of the dendra2 protein, tagged to mitochondrial cytochrome c.
The article describes using confocal microscopy together with free-to-use ImageJ (Fiji) tools to study mitochondrial fission/fusion events using a photoswitchable Dendra2 reporter tagged to mitochondrial cytochrome c.
In this article, we describe the use of confocal microscopy combined with free-to-use tools in ImageJ (Fiji) to study fission/fusion events using the photo-switching property of the dendra2 protein, tagged to mitochondrial cytochrome c.
The article describes using confocal microscopy together with free-to-use ImageJ (Fiji) tools to study mitochondrial fission/fusion events using a photoswitchable Dendra2 reporter tagged to mitochondrial cytochrome c.
In this article, we describe the use of confocal microscopy combined with free-to-use tools in ImageJ (Fiji) to study fission/fusion events using the photo-switching property of the dendra2 protein, tagged to mitochondrial cytochrome c.
The article describes using confocal microscopy together with free-to-use ImageJ (Fiji) tools to study mitochondrial fission/fusion events using a photoswitchable Dendra2 reporter tagged to mitochondrial cytochrome c.
In this article, we describe the use of confocal microscopy combined with free-to-use tools in ImageJ (Fiji) to study fission/fusion events using the photo-switching property of the dendra2 protein, tagged to mitochondrial cytochrome c.
The article describes using confocal microscopy together with free-to-use ImageJ (Fiji) tools to study mitochondrial fission/fusion events using a photoswitchable Dendra2 reporter tagged to mitochondrial cytochrome c.
In this article, we describe the use of confocal microscopy combined with free-to-use tools in ImageJ (Fiji) to study fission/fusion events using the photo-switching property of the dendra2 protein, tagged to mitochondrial cytochrome c.
The article describes using confocal microscopy together with free-to-use ImageJ (Fiji) tools to study mitochondrial fission/fusion events using a photoswitchable Dendra2 reporter tagged to mitochondrial cytochrome c.
In this article, we describe the use of confocal microscopy combined with free-to-use tools in ImageJ (Fiji) to study fission/fusion events using the photo-switching property of the dendra2 protein, tagged to mitochondrial cytochrome c.
The article describes using confocal microscopy together with free-to-use ImageJ (Fiji) tools to study mitochondrial fission/fusion events using a photoswitchable Dendra2 reporter tagged to mitochondrial cytochrome c.
In this article, we describe the use of confocal microscopy combined with free-to-use tools in ImageJ (Fiji) to study fission/fusion events using the photo-switching property of the dendra2 protein, tagged to mitochondrial cytochrome c.
The article describes using confocal microscopy together with free-to-use ImageJ (Fiji) tools to study mitochondrial fission/fusion events using a photoswitchable Dendra2 reporter tagged to mitochondrial cytochrome c.
In this article, we describe the use of confocal microscopy combined with free-to-use tools in ImageJ (Fiji) to study fission/fusion events using the photo-switching property of the dendra2 protein, tagged to mitochondrial cytochrome c.
Approval Evidence
2 sources1 linked approval claimfirst-pass slugs dendra2, mitochondrial-cytochrome-c-tagged-dendra2-reporter
In this article, we describe the use of confocal microscopy combined with free-to-use tools in ImageJ (Fiji) to study fission/fusion events using the photo-switching property of the dendra2 protein, tagged to mitochondrial cytochrome c.
Source:
The supplied web research summary identifies Dendra2 as a strongly supported component-level lead directly relevant to the anchor review's discussion of photoconvertible/photoactivatable fluorescent proteins for superresolution imaging.
Source:
method descriptionsupports
The article describes using confocal microscopy together with free-to-use ImageJ (Fiji) tools to study mitochondrial fission/fusion events using a photoswitchable Dendra2 reporter tagged to mitochondrial cytochrome c.
In this article, we describe the use of confocal microscopy combined with free-to-use tools in ImageJ (Fiji) to study fission/fusion events using the photo-switching property of the dendra2 protein, tagged to mitochondrial cytochrome c.
Source:
Comparisons
Source-backed strengths
A key strength is compatibility with confocal microscopy and Fiji/ImageJ-based analysis, which supports an accessible imaging workflow. The source explicitly describes its application to studying mitochondrial fission/fusion events, but does not provide quantitative performance metrics in the supplied evidence.
Ranked Citations
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Extracted from this source document.