Source 1primary paper2023MED
Toolkit/mMORp
mMORp
Also known as: mouse Oprm1 promoter constructs
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
MORp constructs designed from promoter regions upstream of the mouse Oprm1 gene (mMORp) were validated for transduction efficiency and selectivity in endogenous MOR+ neurons
Usefulness & Problems
Why this is useful
mMORp is a murine Oprm1 promoter-driven viral construct set used to drive transgene expression in endogenous MOR+ neurons. The abstract presents it as a selective genetic-access tool.; selective transduction of endogenous MOR+ neurons; in vivo fiber photometry; behavioral chemogenetics; intersectional genetic strategies
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mMORp is a murine Oprm1 promoter-driven viral construct set used to drive transgene expression in endogenous MOR+ neurons. The abstract presents it as a selective genetic-access tool.
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selective transduction of endogenous MOR+ neurons
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in vivo fiber photometry
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behavioral chemogenetics
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intersectional genetic strategies
Problem solved
It provides a way to access and manipulate MOR+ neurons in tissues relevant to pain, analgesia, and addiction research.; genetic access to endogenous MOR+ neurons in mouse and other tested systems
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It provides a way to access and manipulate MOR+ neurons in tissues relevant to pain, analgesia, and addiction research.
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genetic access to endogenous MOR+ neurons in mouse and other tested systems
Problem links
genetic access to endogenous MOR+ neurons in mouse and other tested systems
LiteratureIt provides a way to access and manipulate MOR+ neurons in tissues relevant to pain, analgesia, and addiction research.
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It provides a way to access and manipulate MOR+ neurons in tissues relevant to pain, analgesia, and addiction research.
Published Workflows
Objective: Develop viral promoter-driven constructs that provide cell type specific genetic access to MOR+/OPRM1+ cells across species and translational models.
Why it works: The workflow uses promoter regions from Oprm1/OPRM1 to bias transgene expression toward MOR-expressing cells, then validates efficiency, selectivity, and application utility across multiple biological systems.
promoter-driven transgene expression in MOR+/OPRM1+ cellsAAV packagingcross-species validationfunctional application testing
Stages
- 1.MOR promoter construct design and AAV packaging(library_design)
This stage creates promoter-driven viral constructs intended to provide genetic access to MOR-expressing cells.
Selection: Promoter regions upstream of mouse Oprm1 or human OPRM1 were used to design constructs intended to drive expression in MOR+/OPRM1+ cells.
- 2.Mouse validation of transduction efficiency and selectivity(confirmatory_validation)
This stage confirms that the murine promoter constructs work in endogenous MOR+ neurons in relevant mouse tissues before broader application.
Selection: Transduction efficiency and selectivity in endogenous MOR+ neurons in mouse brain, spinal cord, and periphery.
- 3.Cross-species and translational expression assessment(secondary_characterization)
This stage tests whether the murine promoter constructs extend beyond mouse into other vertebrate and human-derived systems.
Selection: Robust expression in rats, shrews, and human iPSC-derived nociceptors.
- 4.Functional application demonstration(functional_characterization)
This stage demonstrates that the constructs are not only selective but also useful in downstream neuroscience applications.
Selection: Usability for in vivo fiber photometry, behavioral chemogenetics, and intersectional genetic strategies.
- 5.Primate validation of human-designed promoter construct(confirmatory_validation)
This stage tests a human-designed promoter construct in a primate cortical context to extend the toolkit toward translational use.
Selection: Efficient transduction of macaque cortical OPRM1+ cells by hMORp.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: payload burdenimplementation constraint: spectral hardware requirementoperating role: actuator
It requires the mouse Oprm1 promoter-derived construct and viral delivery. Demonstrated applications also require compatible photometry, chemogenetic, or intersectional genetic setups.; designed from promoter regions upstream of the mouse Oprm1 gene; used as viral constructs
The abstract does not establish universal performance in all tissues, species, or MOR-expressing cell types.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
mMORp was demonstrated for in vivo fiber photometry, behavioral chemogenetics, and intersectional genetic strategies.
The use of mMORp for in vivo fiber photometry, behavioral chemogenetics, and intersectional genetic strategies is also demonstrated.
mMORp showed robust expression in rats, shrews, and human iPSC-derived nociceptors.
additional studies revealing robust expression in rats, shrews, and human induced pluripotent stem cell (iPSC)-derived nociceptors
The authors developed a catalog of MOR promoter based constructs packaged into AAV vectors that drive transgene expression in MOR+ cells.
we developed a catalog of MOR promoter (MORp) based constructs packaged into adeno-associated viral vectors that drive transgene expression in MOR+ cells
hMORp efficiently transduced macaque cortical OPRM1+ cells.
a human designed MORp (hMORp) efficiently transduced macaque cortical OPRM1+ cells
mMORp constructs were validated for transduction efficiency and selectivity in endogenous MOR+ neurons in the brain, spinal cord, and periphery of mice.
mMORp constructs designed from promoter regions upstream of the mouse Oprm1 gene (mMORp) were validated for transduction efficiency and selectivity in endogenous MOR+ neurons in the brain, spinal cord, and periphery of mice
Approval Evidence
1 source3 linked approval claimsfirst-pass slug mmorp
MORp constructs designed from promoter regions upstream of the mouse Oprm1 gene (mMORp) were validated for transduction efficiency and selectivity in endogenous MOR+ neurons
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application demosupports
mMORp was demonstrated for in vivo fiber photometry, behavioral chemogenetics, and intersectional genetic strategies.
The use of mMORp for in vivo fiber photometry, behavioral chemogenetics, and intersectional genetic strategies is also demonstrated.
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cross species applicabilitysupports
mMORp showed robust expression in rats, shrews, and human iPSC-derived nociceptors.
additional studies revealing robust expression in rats, shrews, and human induced pluripotent stem cell (iPSC)-derived nociceptors
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validation resultsupports
mMORp constructs were validated for transduction efficiency and selectivity in endogenous MOR+ neurons in the brain, spinal cord, and periphery of mice.
mMORp constructs designed from promoter regions upstream of the mouse Oprm1 gene (mMORp) were validated for transduction efficiency and selectivity in endogenous MOR+ neurons in the brain, spinal cord, and periphery of mice
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Comparisons
Source-stated alternatives
Comparator endogenous-locus access strategies mentioned in the upstream summary include Oprm1-Cre and MOR-CreER mouse lines.
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Comparator endogenous-locus access strategies mentioned in the upstream summary include Oprm1-Cre and MOR-CreER mouse lines.
Source-backed strengths
validated in brain, spinal cord, and periphery of mice; robust expression reported in rats, shrews, and human iPSC-derived nociceptors
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validated in brain, spinal cord, and periphery of mice
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robust expression reported in rats, shrews, and human iPSC-derived nociceptors
mMORp and cell-specific receptor subtype gene deletion mouse models address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with optogenetic probes
mMORp and optogenetic probes address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with organoid fusion
mMORp and organoid fusion address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.