Source 1primary paper2013Nucleic Acids Research
Toolkit/multi-chromatic multi-gene control system
multi-chromatic multi-gene control system
Also known as: portfolio of optogenetic tools
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The multi-chromatic multi-gene control system is a combined optogenetic platform for mammalian cells that integrates UVB-, blue-, and red-light-inducible gene control modules. It enables differential induction of up to three genes within a single cell culture using distinct wavelengths and was applied to gene expression and signaling control.
Usefulness & Problems
Why this is useful
This system is useful for wavelength-addressable control of multiple genetic programs in the same mammalian cell culture. The cited study used it for mammalian gene expression and angiogenic signaling processes, indicating utility where coordinated but separable optical inputs are needed.
Source:
This combined optogenetic system differentially induces up to three genes in a single mammalian cell culture using different wavelengths of light. It is presented as a multi-chromatic control platform for gene expression and signaling.
Source:
differential expression of up to three genes in one mammalian cell culture
Source:
multi-chromatic control of angiogenic signaling processes
Source:
design of synthetic biological networks with spatiotemporal precision
Problem solved
It addresses the problem of controlling multiple genes independently within one mammalian cell culture using nonidentical light inputs. The evidence specifically supports differential induction of up to three genes in response to different wavelengths.
Source:
It solves the problem of coordinating complementary gene expression and signaling processes in a predictable, wavelength-addressable way within mammalian cells.
Source:
enables wavelength-specific control of multiple genes in the same culture
Problem links
enables wavelength-specific control of multiple genes in the same culture
LiteratureIt solves the problem of coordinating complementary gene expression and signaling processes in a predictable, wavelength-addressable way within mammalian cells.
Source:
It solves the problem of coordinating complementary gene expression and signaling processes in a predictable, wavelength-addressable way within mammalian cells.
Published Workflows
Objective: Develop and combine wavelength-specific optogenetic gene control systems to achieve predictable multi-chromatic control of mammalian gene expression and signaling.
Why it works: The workflow combines orthogonal light-responsive control channels so that different wavelengths can independently regulate different gene outputs in the same mammalian cell culture.
light-responsive split transcription factor activationdifferential gene induction by distinct wavelengthssynthetic system designquantitative modelingsystem combination
Stages
- 1.UVB-responsive system design(library_design)
To create a new UVB-inducible control channel for mammalian gene expression.
Selection: Design of a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1
- 2.Mammalian cell characterization of UVB induction(functional_characterization)
To establish that the engineered UVB system functions strongly in mammalian cells.
Selection: Magnitude of UVB-induced gene expression across mammalian cells
- 3.Quantitative parameter determination(secondary_characterization)
To identify critical parameters that govern system behavior.
Selection: Determination of critical system parameters using a quantitative model
- 4.Combination into multi-chromatic control(confirmatory_validation)
To show that the UVB module can be integrated with blue and red systems for multi-chromatic control.
Selection: Ability to differentially express three genes in one mammalian cell culture using different wavelengths
- 5.Angiogenic signaling application(confirmatory_validation)
To demonstrate that the multi-chromatic control system can regulate a biologically relevant signaling process.
Selection: Application of the multi-chromatic system to control angiogenic signaling processes
Steps
- 1.Design UVB-responsive split transcription factorengineered expression system
Create a UVB-inducible gene expression module for mammalian cells.
A UVB-responsive control channel had to be engineered before it could be characterized or combined with other light-responsive systems.
- 2.Measure UVB-induced gene expression across mammalian cellssystem being characterized
Establish inducibility and host-range performance of the UVB-responsive system.
The newly designed system needed functional characterization before broader integration into a multi-color platform.
- 3.Determine critical system parameters using a quantitative modelanalysis method applied to engineered system
Identify critical parameters governing system behavior.
After establishing that the system functions, modeling supports quantitative understanding needed for predictable use and integration.
- 4.Combine UVB, blue, and red light-inducible systems for multi-gene controlcombined control platform
Build a multi-chromatic system capable of differential control of multiple genes in one culture.
The UVB module was combined with existing blue and red systems only after it had been developed and characterized.
- 5.Apply the multi-chromatic system to angiogenic signaling controlsystem being applied
Demonstrate functional control of a biologically relevant signaling process.
After showing multi-gene control, the authors tested whether the platform could control angiogenic signaling processes.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
Computational DesignTarget processes
signalingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: split
Implementation requires combining a UVB-responsive module with blue- and red-light-inducible gene control technologies in mammalian cell expression systems. Practical use therefore depends on delivery of multiple wavelength-specific light inputs and construct compatibility within the same cell culture, but the provided evidence does not specify cofactors or exact construct design.
The supplied evidence only supports use in mammalian cell culture and does not establish performance in vivo, clinical settings, or across other organisms. The available text also does not define quantitative performance metrics, molecular architecture, or scalability beyond three genes.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
genes differentially induced 3
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
The authors developed a UVB-inducible expression system by designing a UVB-responsive split transcription factor based on UVR8 and the WD40 domain of COP1.
we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
A quantitative model was used to determine critical system parameters.
Based on a quantitative model, we determined critical system parameters.
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
The UVB-inducible expression system enabled up to 800-fold UVB-induced gene expression in human, monkey, hamster, and mouse cells.
The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells.
UVB-induced gene expression fold induction 800 fold
Approval Evidence
1 source3 linked approval claimsfirst-pass slug multi-chromatic-multi-gene-control-system
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells
Source:
applicationsupports
Combining the UVB-responsive system with blue and red light-inducible gene control technology enabled multi-chromatic multi-gene control and was applied to angiogenic signaling processes.
By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes.
Source:
capabilitysupports
The study demonstrates multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to different wavelengths of light.
we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths
Source:
impact statementsupports
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks with unmatched spatiotemporal precision for future research and biomedical applications.
This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications.
Source:
Comparisons
Source-stated alternatives
The abstract mentions the individual blue and red light-inducible gene control technologies as component approaches that are combined with the UVB system.
Source:
The abstract mentions the individual blue and red light-inducible gene control technologies as component approaches that are combined with the UVB system.
Source-backed strengths
The reported strength is spectral multiplexing across UVB, blue, and red light-inducible gene control technologies in mammalian cells. The study demonstrated differential expression of three genes in a single culture and applied the approach to signaling, including angiogenic signaling processes.
Source:
supports up to three independently light-addressable gene outputs
Source:
described as enabling unmatched spatiotemporal precision
Compared with CfRhPDE1
multi-chromatic multi-gene control system and CfRhPDE1 address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling; same primary input modality: light
Compared with NIR Rac1 biosensor
multi-chromatic multi-gene control system and NIR Rac1 biosensor address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling; same primary input modality: light
Compared with photobiomodulation therapy
multi-chromatic multi-gene control system and photobiomodulation therapy address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling; same primary input modality: light
Ranked Citations
- 1.