Source 1primary paper2026MED
Toolkit/multiparametric imaging system
multiparametric imaging system
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here, we present a multiparametric imaging system that simultaneously records four wavelengths to visualize cAMP activity alongside real-time transmembrane potential and intracellular Ca2+ in intact hearts from a cardiac-specific cAMP reporter mouse.
Usefulness & Problems
Why this is useful
This system simultaneously records four wavelengths to visualize cAMP activity together with real-time transmembrane potential and intracellular Ca2+ in intact hearts. It is presented as a way to connect signaling dynamics with tissue-level cardiac function.; simultaneous imaging of cAMP activity, transmembrane potential, and intracellular Ca2+ in intact hearts; linking cellular signaling to tissue-level cardiac function; high spatiotemporal resolution study of cardiac signaling and electrophysiology
Source:
This system simultaneously records four wavelengths to visualize cAMP activity together with real-time transmembrane potential and intracellular Ca2+ in intact hearts. It is presented as a way to connect signaling dynamics with tissue-level cardiac function.
Source:
simultaneous imaging of cAMP activity, transmembrane potential, and intracellular Ca2+ in intact hearts
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linking cellular signaling to tissue-level cardiac function
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high spatiotemporal resolution study of cardiac signaling and electrophysiology
Problem solved
It addresses the limitation that cellular signaling measurements, especially cAMP reporter readouts, are hard to link to tissue-level function in the intact heart.; limited ability to connect cellular cAMP signaling measurements to tissue-level function in intact heart preparations
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It addresses the limitation that cellular signaling measurements, especially cAMP reporter readouts, are hard to link to tissue-level function in the intact heart.
Source:
limited ability to connect cellular cAMP signaling measurements to tissue-level function in intact heart preparations
Problem links
limited ability to connect cellular cAMP signaling measurements to tissue-level function in intact heart preparations
LiteratureIt addresses the limitation that cellular signaling measurements, especially cAMP reporter readouts, are hard to link to tissue-level function in the intact heart.
Source:
It addresses the limitation that cellular signaling measurements, especially cAMP reporter readouts, are hard to link to tissue-level function in the intact heart.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The abstract states that the system is used in intact hearts from a cardiac-specific cAMP reporter mouse. It also requires simultaneous optical recording of four wavelength channels.; requires intact hearts from a cardiac-specific cAMP reporter mouse
The abstract does not show that the system by itself establishes causality or generalizes beyond the reported intact-heart reporter-mouse setting.; described in the abstract only for intact hearts from a cardiac-specific cAMP reporter mouse
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
In response to pharmacological or nerve-activated beta-adrenergic receptor stimulation, cAMP is strongly and heterogeneously activated throughout atria and ventricles in the intact heart.
The time course of cAMP responses matches heart rate, action potential, and intracellular calcium responses in the intact heart under beta-adrenergic receptor stimulation.
The multiparametric imaging system simultaneously records four wavelengths to visualize cAMP activity together with real-time transmembrane potential and intracellular calcium in intact hearts from a cardiac-specific cAMP reporter mouse.
wavelengths recorded simultaneously 4
Approval Evidence
1 source3 linked approval claimsfirst-pass slug multiparametric-imaging-system
Here, we present a multiparametric imaging system that simultaneously records four wavelengths to visualize cAMP activity alongside real-time transmembrane potential and intracellular Ca2+ in intact hearts from a cardiac-specific cAMP reporter mouse.
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biological observationsupports
In response to pharmacological or nerve-activated beta-adrenergic receptor stimulation, cAMP is strongly and heterogeneously activated throughout atria and ventricles in the intact heart.
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temporal correlationsupports
The time course of cAMP responses matches heart rate, action potential, and intracellular calcium responses in the intact heart under beta-adrenergic receptor stimulation.
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tool capabilitysupports
The multiparametric imaging system simultaneously records four wavelengths to visualize cAMP activity together with real-time transmembrane potential and intracellular calcium in intact hearts from a cardiac-specific cAMP reporter mouse.
Source:
Comparisons
Source-stated alternatives
The abstract contrasts this approach with prior studies in isolated cardiomyocytes using FRET-based reporters, which do not directly link signaling to tissue-level function as effectively.
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The abstract contrasts this approach with prior studies in isolated cardiomyocytes using FRET-based reporters, which do not directly link signaling to tissue-level function as effectively.
Source-backed strengths
simultaneously records four wavelengths; captures cAMP, transmembrane potential, and intracellular Ca2+ together in intact hearts; supports high spatiotemporal resolution measurements
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simultaneously records four wavelengths
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captures cAMP, transmembrane potential, and intracellular Ca2+ together in intact hearts
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supports high spatiotemporal resolution measurements
Compared with FRET
The abstract contrasts this approach with prior studies in isolated cardiomyocytes using FRET-based reporters, which do not directly link signaling to tissue-level function as effectively.
Shared frame: source-stated alternative in extracted literature
Strengths here: simultaneously records four wavelengths; captures cAMP, transmembrane potential, and intracellular Ca2+ together in intact hearts; supports high spatiotemporal resolution measurements.
Relative tradeoffs: described in the abstract only for intact hearts from a cardiac-specific cAMP reporter mouse.
Source:
The abstract contrasts this approach with prior studies in isolated cardiomyocytes using FRET-based reporters, which do not directly link signaling to tissue-level function as effectively.
Ranked Citations
- 1.