Source 1review2021Viruses
Toolkit/NCBI sequence screening for 2A/2A-like occurrence
NCBI sequence screening for 2A/2A-like occurrence
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
NCBI sequence screening for 2A/2A-like occurrence is a computational survey method that updates the distribution of viral 2A and 2A-like sequences by screening sequences deposited in the National Center for Biotechnology Information database. In the cited 2021 review, this approach identified 69 newly reported 2A-like occurrences across multiple virus groups.
Usefulness & Problems
Why this is useful
This method is useful for mapping the taxonomic distribution of 2A and 2A-like sequences that are relevant because such sequences have been used in heterologous co-expression systems to cleave proteins of biotechnological interest. It supports identification of newly reported occurrences in public sequence repositories, expanding the known sequence space for these elements.
Source:
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
Problem solved
It addresses the problem of incomplete and outdated knowledge about where 2A and 2A-like sequences occur across viruses. By screening NCBI sequence records, the method updates occurrence data and reveals newly reported examples across positive-sense single-stranded RNA, double-stranded RNA, and negative-sense single-stranded RNA viruses.
Source:
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
Problem links
Need better screening or enrichment leverage
DerivedNCBI sequence screening for 2A/2A-like occurrence is a computational survey method that reviews and updates the occurrence of viral 2A and 2A-like sequences by screening sequences available in the National Center for Biotechnology Information database. In the cited study, this approach identified newly reported 2A-like occurrences across multiple viral groups.
Need conditional recombination or state switching
DerivedNCBI sequence screening for 2A/2A-like occurrence is a computational survey method that reviews and updates the occurrence of viral 2A and 2A-like sequences by screening sequences available in the National Center for Biotechnology Information database. In the cited study, this approach identified newly reported 2A-like occurrences across multiple viral groups.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete computational method used to design, rank, or analyze an engineered system.
Mechanisms
No mechanism tags yet.
Target processes
recombinationselectionImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenoperating role: builderswitch architecture: multi component
Implementation is described only as screening sequences available in the National Center for Biotechnology Information database for 2A/2A-like occurrences. The evidence does not specify query design, motif definitions, software pipeline, scoring thresholds, or downstream validation workflow.
The supplied evidence supports database screening for occurrence mapping, but it does not describe the search algorithm, inclusion criteria, sensitivity, specificity, or experimental validation of identified sequences. No evidence is provided for performance benchmarking, independent replication, or direct functional testing of the newly identified 2A-like sequences within this method.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
2A and 2A-like sequences have been used in heterologous co-expression systems as versatile tools for cleaving proteins of biotechnological interest.
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
Among the 69 newly reported 2A-like occurrences, 62 were in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
double-stranded RNA virus count 6negative-sense single-stranded RNA virus count 1positive-sense single-stranded RNA species count 62
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A and 2A-like sequences are widely distributed across viruses from insect to mammalian viruses.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
2A is an oligopeptide sequence that mediates ribosome skipping and can mediate co-translation cleavage of polyproteins.
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
newly reported 2A-like sequences 69
Approval Evidence
1 source1 linked approval claimfirst-pass slug ncbi-sequence-screening-for-2a-2a-like-occurrence
we review and update the occurrence of 2A/2A-like sequences in different groups of viruses by screening the sequences available in the National Center for Biotechnology Information database
Source:
review findingsupports
The review reports first-time occurrence of 2A-like sequences in 69 sequences identified by screening NCBI sequence records.
Source:
Comparisons
Source-backed strengths
The cited application reported 69 newly reported 2A-like occurrences, indicating that the approach can recover substantial additional distributional information from public databases. The reported breakdown was 62 occurrences in positive-sense single-stranded RNA species, 6 in double-stranded RNA viruses, and 1 in a negative-sense single-stranded RNA virus.
Compared with FRASE
NCBI sequence screening for 2A/2A-like occurrence and FRASE address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection
Relative tradeoffs: looks easier to implement in practice.
Compared with FRASE-bot
NCBI sequence screening for 2A/2A-like occurrence and FRASE-bot address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with SIBR-Cas
NCBI sequence screening for 2A/2A-like occurrence and SIBR-Cas address a similar problem space because they share recombination, selection.
Shared frame: shared target processes: recombination, selection
Ranked Citations
- 1.