Source 1primary paper2026MED
Toolkit/NIR light-activated CRISPR-dCas9/Cas9 system
NIR light-activated CRISPR-dCas9/Cas9 system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The NIR light-activated CRISPR-dCas9/Cas9 system is a multi-component optogenetic platform that controls CRISPR-dCas9/Cas9 gene regulation and editing with near-infrared light. It uses a chemically cleavable rapamycin dimer to confer precise and rapid light-dependent activity in living organisms.
Usefulness & Problems
Why this is useful
This platform is useful for noninvasive, spatially confined control of CRISPR-based gene regulation and editing in vivo. The source positions it as a potentially preclinical and clinically translatable approach for targeted genome engineering.
Source:
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
Source:
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
Problem solved
It addresses the problem of achieving targeted and temporally controlled CRISPR-dCas9/Cas9 activity in living organisms using an external light input. The reported design specifically aims to provide near-infrared-triggered, spatially confined activation through a chemically cleavable rapamycin dimer.
Source:
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
Problem links
Need controllable genome or transcript editing
DerivedThe NIR light-activated CRISPR-dCas9/Cas9 system is a multi-component optogenetic gene regulation and editing platform controlled by near-infrared light through a chemically cleavable rapamycin dimer. It is reported to enable precise and rapid CRISPR-dCas9/Cas9 activity in living organisms.
Need precise spatiotemporal control with light input
DerivedThe NIR light-activated CRISPR-dCas9/Cas9 system is a multi-component optogenetic gene regulation and editing platform controlled by near-infrared light through a chemically cleavable rapamycin dimer. It is reported to enable precise and rapid CRISPR-dCas9/Cas9 activity in living organisms.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
HeterodimerizationHeterodimerizationHeterodimerizationPhotocleavagePhotocleavagePhotocleavageTechniques
No technique tags yet.
Target processes
editingInput: Light
Implementation Constraints
activation chemistry: chemically cleavable rapamycin dimercofactor dependency: cofactor requirement unknowneffector family: CRISPR-dCas9/Cas9encoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementmodality: near infrared light controloperating role: regulatorswitch architecture: cleavageswitch architecture: multi componentswitch architecture: recruitment
Implementation involves a multi-component CRISPR-dCas9/Cas9 system controlled by near-infrared light and a chemically cleavable rapamycin dimer. The provided evidence does not specify the exact protein fusions, illumination parameters, delivery format, or required cofactors.
The supplied evidence does not provide quantitative performance metrics, specific editing outcomes, or direct comparative benchmarks against other optogenetic CRISPR systems. Independent replication, detailed construct architecture, and the extent of validation across cell types or organisms are not described in the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug nir-light-activated-crispr-dcas9-cas9-system
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
Source:
application potentialsupports
The platform is positioned for highly efficient, targeted, noninvasive, and spatially confined gene editing with potential preclinical and clinically translatable applications.
This platform opens new directions for highly efficient, targeted, noninvasive, and spatially confined gene editing for a great number of preclinical and clinically translatable applications.
Source:
capabilitysupports
The reported NIR light-activated CRISPR-dCas9/Cas9 system enables precise and rapid gene regulation in living organisms using a chemically cleavable rapamycin dimer.
A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer.
Source:
comparative advantagesupports
Compared with previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Source:
Comparisons
Source-backed strengths
Reported strengths include precise and rapid gene regulation in living organisms under near-infrared illumination. The source also describes the system as highly efficient, targeted, noninvasive, and spatially confined for gene editing applications.
Source:
Unlike previous light-driven systems, this approach offers deeper tissue penetration, low toxicity, fast response, and minimal background activity.
Compared with LITEs (Light-inducible transcriptional effectors)
NIR light-activated CRISPR-dCas9/Cas9 system and LITEs (Light-inducible transcriptional effectors) address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; shared mechanisms: heterodimerization; same primary input modality: light
Compared with photoactivatable nanoCRISPR/Cas9 system
NIR light-activated CRISPR-dCas9/Cas9 system and photoactivatable nanoCRISPR/Cas9 system address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; shared mechanisms: photocleavage; same primary input modality: light
Compared with photoactivated CRISPR/Cas12a strategy
NIR light-activated CRISPR-dCas9/Cas9 system and photoactivated CRISPR/Cas12a strategy address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; shared mechanisms: photocleavage; same primary input modality: light
Ranked Citations
- 1.