Source 1primary paper2022
Toolkit/nMag/pMag photodimerization system
nMag/pMag photodimerization system
Also known as: Magnets photosensors
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The nMag/pMag photodimerization system, also called Magnets photosensors, is a light-controlled protein-domain pair that mediates heterodimerization. Reported engineering work altered its light sensitivity and tuned its light-activity dose-response behavior through directed evolution and high-throughput screening.
Usefulness & Problems
Why this is useful
This system is useful for light-dependent control of protein association, and the reported engineered variants improve activity at low light intensities. In the cited transfer to mOptoT7, increased gene expression at low light was associated with reduced potential phototoxicity in long-term experiments.
Problem solved
The reported work addresses the problem of insufficient or poorly tuned photosensitivity in the nMag/pMag system. It also addresses the need to independently tune photosensitivity and expression output so that the light-response curve can be adjusted for specific applications.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Mechanisms
HeterodimerizationTarget processes
recombinationselectionInput: Light
Implementation Constraints
The reported optimization used directed evolution together with high-throughput screening to identify altered variants. Practical implementation details such as construct design, expression system, cofactors, and illumination parameters are not provided in the supplied evidence, although transfer into mOptoT7 is explicitly mentioned.
The supplied evidence does not specify the molecular architecture, chromophore requirements, illumination wavelength, or host range of the nMag/pMag pair. Validation in the provided evidence is limited to the cited engineering study and a transferred mOptoT7 context, with no independent replication described.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The authors developed and applied a directed evolution and high-throughput screening strategy to alter the light sensitivity of the nMag/pMag photodimerization system.
we develop and apply a simple, yet powerful, directed evolution and high-throughput screening strategy that allows us to alter the most fundamental property of the widely used nMag/pMag photodimerization system: its light sensitivity
The authors developed and applied a directed evolution and high-throughput screening strategy to alter the light sensitivity of the nMag/pMag photodimerization system.
we develop and apply a simple, yet powerful, directed evolution and high-throughput screening strategy that allows us to alter the most fundamental property of the widely used nMag/pMag photodimerization system: its light sensitivity
The authors developed and applied a directed evolution and high-throughput screening strategy to alter the light sensitivity of the nMag/pMag photodimerization system.
we develop and apply a simple, yet powerful, directed evolution and high-throughput screening strategy that allows us to alter the most fundamental property of the widely used nMag/pMag photodimerization system: its light sensitivity
The authors developed and applied a directed evolution and high-throughput screening strategy to alter the light sensitivity of the nMag/pMag photodimerization system.
we develop and apply a simple, yet powerful, directed evolution and high-throughput screening strategy that allows us to alter the most fundamental property of the widely used nMag/pMag photodimerization system: its light sensitivity
The authors developed and applied a directed evolution and high-throughput screening strategy to alter the light sensitivity of the nMag/pMag photodimerization system.
we develop and apply a simple, yet powerful, directed evolution and high-throughput screening strategy that allows us to alter the most fundamental property of the widely used nMag/pMag photodimerization system: its light sensitivity
The authors developed and applied a directed evolution and high-throughput screening strategy to alter the light sensitivity of the nMag/pMag photodimerization system.
we develop and apply a simple, yet powerful, directed evolution and high-throughput screening strategy that allows us to alter the most fundamental property of the widely used nMag/pMag photodimerization system: its light sensitivity
The authors developed and applied a directed evolution and high-throughput screening strategy to alter the light sensitivity of the nMag/pMag photodimerization system.
we develop and apply a simple, yet powerful, directed evolution and high-throughput screening strategy that allows us to alter the most fundamental property of the widely used nMag/pMag photodimerization system: its light sensitivity
Transferred variants in mOptoT7 increased gene expression levels at low light intensities, which the authors state results in reduced potential phototoxicity in long-term experiments.
We demonstrate increased gene expression levels for low light intensities, resulting in reduced potential phototoxicity in long-term experiments.
Transferred variants in mOptoT7 increased gene expression levels at low light intensities, which the authors state results in reduced potential phototoxicity in long-term experiments.
We demonstrate increased gene expression levels for low light intensities, resulting in reduced potential phototoxicity in long-term experiments.
Transferred variants in mOptoT7 increased gene expression levels at low light intensities, which the authors state results in reduced potential phototoxicity in long-term experiments.
We demonstrate increased gene expression levels for low light intensities, resulting in reduced potential phototoxicity in long-term experiments.
Transferred variants in mOptoT7 increased gene expression levels at low light intensities, which the authors state results in reduced potential phototoxicity in long-term experiments.
We demonstrate increased gene expression levels for low light intensities, resulting in reduced potential phototoxicity in long-term experiments.
Transferred variants in mOptoT7 increased gene expression levels at low light intensities, which the authors state results in reduced potential phototoxicity in long-term experiments.
We demonstrate increased gene expression levels for low light intensities, resulting in reduced potential phototoxicity in long-term experiments.
Transferred variants in mOptoT7 increased gene expression levels at low light intensities, which the authors state results in reduced potential phototoxicity in long-term experiments.
We demonstrate increased gene expression levels for low light intensities, resulting in reduced potential phototoxicity in long-term experiments.
Transferred variants in mOptoT7 increased gene expression levels at low light intensities, which the authors state results in reduced potential phototoxicity in long-term experiments.
We demonstrate increased gene expression levels for low light intensities, resulting in reduced potential phototoxicity in long-term experiments.
For some variants, photosensitivity and expression levels could be changed independently, enabling tuning of the light-activity dose-response curve.
For some of these variants, photosensitivity and expression levels could be changed independently, showing that the shape of the light-activity dose-response curve can be tuned and adjusted.
For some variants, photosensitivity and expression levels could be changed independently, enabling tuning of the light-activity dose-response curve.
For some of these variants, photosensitivity and expression levels could be changed independently, showing that the shape of the light-activity dose-response curve can be tuned and adjusted.
For some variants, photosensitivity and expression levels could be changed independently, enabling tuning of the light-activity dose-response curve.
For some of these variants, photosensitivity and expression levels could be changed independently, showing that the shape of the light-activity dose-response curve can be tuned and adjusted.
For some variants, photosensitivity and expression levels could be changed independently, enabling tuning of the light-activity dose-response curve.
For some of these variants, photosensitivity and expression levels could be changed independently, showing that the shape of the light-activity dose-response curve can be tuned and adjusted.
For some variants, photosensitivity and expression levels could be changed independently, enabling tuning of the light-activity dose-response curve.
For some of these variants, photosensitivity and expression levels could be changed independently, showing that the shape of the light-activity dose-response curve can be tuned and adjusted.
For some variants, photosensitivity and expression levels could be changed independently, enabling tuning of the light-activity dose-response curve.
For some of these variants, photosensitivity and expression levels could be changed independently, showing that the shape of the light-activity dose-response curve can be tuned and adjusted.
For some variants, photosensitivity and expression levels could be changed independently, enabling tuning of the light-activity dose-response curve.
For some of these variants, photosensitivity and expression levels could be changed independently, showing that the shape of the light-activity dose-response curve can be tuned and adjusted.
Mutations within the photosensory domains were identified that increase or decrease light sensitivity at sub-saturating light intensities, and some variants also improve dark-to-light fold change.
We identify a set of mutations located within the photosensory domains, which either increase or decrease the light sensitivity at sub-saturating light intensities, while also improving the dark-to-light fold change in certain variants.
Mutations within the photosensory domains were identified that increase or decrease light sensitivity at sub-saturating light intensities, and some variants also improve dark-to-light fold change.
We identify a set of mutations located within the photosensory domains, which either increase or decrease the light sensitivity at sub-saturating light intensities, while also improving the dark-to-light fold change in certain variants.
Mutations within the photosensory domains were identified that increase or decrease light sensitivity at sub-saturating light intensities, and some variants also improve dark-to-light fold change.
We identify a set of mutations located within the photosensory domains, which either increase or decrease the light sensitivity at sub-saturating light intensities, while also improving the dark-to-light fold change in certain variants.
Mutations within the photosensory domains were identified that increase or decrease light sensitivity at sub-saturating light intensities, and some variants also improve dark-to-light fold change.
We identify a set of mutations located within the photosensory domains, which either increase or decrease the light sensitivity at sub-saturating light intensities, while also improving the dark-to-light fold change in certain variants.
Mutations within the photosensory domains were identified that increase or decrease light sensitivity at sub-saturating light intensities, and some variants also improve dark-to-light fold change.
We identify a set of mutations located within the photosensory domains, which either increase or decrease the light sensitivity at sub-saturating light intensities, while also improving the dark-to-light fold change in certain variants.
Mutations within the photosensory domains were identified that increase or decrease light sensitivity at sub-saturating light intensities, and some variants also improve dark-to-light fold change.
We identify a set of mutations located within the photosensory domains, which either increase or decrease the light sensitivity at sub-saturating light intensities, while also improving the dark-to-light fold change in certain variants.
Mutations within the photosensory domains were identified that increase or decrease light sensitivity at sub-saturating light intensities, and some variants also improve dark-to-light fold change.
We identify a set of mutations located within the photosensory domains, which either increase or decrease the light sensitivity at sub-saturating light intensities, while also improving the dark-to-light fold change in certain variants.
A subset of Magnets variants can be transferred into mOptoT7 for gene expression regulation in mammalian cells.
We further show that a subset of these variants can be transferred into the mOptoT7 for gene expression regulation in mammalian cells.
A subset of Magnets variants can be transferred into mOptoT7 for gene expression regulation in mammalian cells.
We further show that a subset of these variants can be transferred into the mOptoT7 for gene expression regulation in mammalian cells.
A subset of Magnets variants can be transferred into mOptoT7 for gene expression regulation in mammalian cells.
We further show that a subset of these variants can be transferred into the mOptoT7 for gene expression regulation in mammalian cells.
A subset of Magnets variants can be transferred into mOptoT7 for gene expression regulation in mammalian cells.
We further show that a subset of these variants can be transferred into the mOptoT7 for gene expression regulation in mammalian cells.
A subset of Magnets variants can be transferred into mOptoT7 for gene expression regulation in mammalian cells.
We further show that a subset of these variants can be transferred into the mOptoT7 for gene expression regulation in mammalian cells.
A subset of Magnets variants can be transferred into mOptoT7 for gene expression regulation in mammalian cells.
We further show that a subset of these variants can be transferred into the mOptoT7 for gene expression regulation in mammalian cells.
A subset of Magnets variants can be transferred into mOptoT7 for gene expression regulation in mammalian cells.
We further show that a subset of these variants can be transferred into the mOptoT7 for gene expression regulation in mammalian cells.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug nmag-pmag-photodimerization-system
the widely used nMag/pMag photodimerization system
Source:
engineering method applicationsupports
The authors developed and applied a directed evolution and high-throughput screening strategy to alter the light sensitivity of the nMag/pMag photodimerization system.
we develop and apply a simple, yet powerful, directed evolution and high-throughput screening strategy that allows us to alter the most fundamental property of the widely used nMag/pMag photodimerization system: its light sensitivity
Source:
property decouplingsupports
For some variants, photosensitivity and expression levels could be changed independently, enabling tuning of the light-activity dose-response curve.
For some of these variants, photosensitivity and expression levels could be changed independently, showing that the shape of the light-activity dose-response curve can be tuned and adjusted.
Source:
property tuningsupports
Mutations within the photosensory domains were identified that increase or decrease light sensitivity at sub-saturating light intensities, and some variants also improve dark-to-light fold change.
We identify a set of mutations located within the photosensory domains, which either increase or decrease the light sensitivity at sub-saturating light intensities, while also improving the dark-to-light fold change in certain variants.
Source:
transferabilitysupports
A subset of Magnets variants can be transferred into mOptoT7 for gene expression regulation in mammalian cells.
We further show that a subset of these variants can be transferred into the mOptoT7 for gene expression regulation in mammalian cells.
Source:
Comparisons
Source-backed strengths
The system is described as widely used, indicating established utility as a photodimerization module. Directed-evolution-derived variants increased gene expression at low light intensities in mOptoT7, and some variants allowed photosensitivity and expression level to be changed independently, enabling dose-response tuning.
Source:
we develop and apply a simple, yet powerful, directed evolution and high-throughput screening strategy that allows us to alter the most fundamental property of the widely used nMag/pMag photodimerization system: its light sensitivity
Source:
We demonstrate increased gene expression levels for low light intensities, resulting in reduced potential phototoxicity in long-term experiments.
Ranked Citations
- 1.