Source 1primary paper2021MED
Toolkit/NP-cIPTG
NP-cIPTG
Also known as: 6-nitropiperonyl-caged IPTG, NP-cIPTG
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
NP-cIPTG, or 6-nitropiperonyl-caged IPTG, is a photocaged small-molecule inducer for light-regulated control of LacI-dependent bacterial gene expression. Illumination releases IPTG activity and enables optochemical induction, including in Rhodobacter capsulatus.
Usefulness & Problems
Why this is useful
This tool enables external, light-based induction of bacterial expression systems without direct genetic photoreceptor engineering. The cited literature describes photocaged IPTG as a well-established optochemical approach for regulating gene expression in bacteria and demonstrates its use to control an endogenous metabolic function in R. capsulatus.
Source:
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Source:
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
Source:
photo-responsive inducer molecules such as 6-nitropiperonyl-caged IPTG (NP-cIPTG) have been used as optochemical tools for Lac repressor-controlled microbial expression systems
Problem solved
NP-cIPTG addresses the problem of achieving spatiotemporally controlled induction of LacI-regulated genes using light rather than conventional bulk chemical addition alone. It also supports optochemical control in bacterial contexts such as the anoxygenic phototroph Rhodobacter capsulatus.
Source:
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Source:
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
Problem links
provides an effective cIPTG variant for light-triggered induction in Rhodobacter capsulatus
LiteratureIt addresses the need for a working light-responsive inducer variant in Rhodobacter capsulatus.
Source:
It addresses the need for a working light-responsive inducer variant in Rhodobacter capsulatus.
provides an optochemical on-switch for Lac repressor-controlled expression systems
LiteratureIt enables non-invasive, tuneable, and spatiotemporally controlled induction compared with conventional chemical induction alone.
Source:
It enables non-invasive, tuneable, and spatiotemporally controlled induction compared with conventional chemical induction alone.
Published Workflows
Objective: Implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using photocaged IPTG variants and demonstrate control of a cellular function.
Why it works: The workflow uses photocaged IPTG as a light-responsive inducer so that illumination can trigger target gene expression, and then applies the same approach to a native cellular function as a demonstration.
light-mediated uncaging of inducer to activate gene expressioninduction of intrinsic carotenoid biosynthesistesting different photocaged inducer variantsoptochemical induction
Stages
- 1.Evaluation of different cIPTG variants in Rhodobacter capsulatus(broad_screen)
The study tested different cIPTG variants to identify a variant that works as a light-mediated on-switch in Rhodobacter capsulatus.
Selection: Ability of cIPTG variants to support light-mediated induction of target gene expression under phototrophic and non-phototrophic cultivation conditions.
- 2.Identification of especially applicable NP-cIPTG variant(hit_picking)
This stage narrows the tested cIPTG variants to the variant highlighted as especially applicable in the host.
Selection: NP-cIPTG was identified as especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
- 3.Functional demonstration via induction of intrinsic carotenoid biosynthesis(confirmatory_validation)
The stage confirms that the optochemical induction approach can be used not only for target gene expression but also to engineer a cellular function.
Selection: Successful induction of intrinsic carotenoid biosynthesis as a showcase of engineering a cellular function.
Objective: Improve the light responsiveness and host applicability of LacI-controlled bacterial expression systems by modulating the water solubility of photocaged IPTG derivatives.
Why it works: The workflow is based on the premise that changing cIPTG water solubility can improve the light responsiveness of bacterial expression systems while preserving the optochemical on-switch behavior of photocaged IPTG.
light-triggered uncaging of IPTG analogs to control LacI-regulated expressionchemical derivative designhost transfer testinglight-mediated gene expression control
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: actuatorswitch architecture: cleavageswitch architecture: uncaging
NP-cIPTG is used in LacI-regulated bacterial expression systems and requires illumination to activate inducer function through uncaging. One cited study specifically concerns the effect of photocaged IPTG solubility on light responsiveness in different bacteria, indicating that solubility is a practical parameter, but the supplied evidence does not specify formulation, illumination settings, or construct architecture.
The supplied evidence does not provide quantitative performance metrics such as induction fold, uncaging efficiency, response kinetics, wavelength dependence, or dark-state leakiness. Validation is limited to a small number of cited studies, and independent replication beyond those reports is not established here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2022MED
Ranked Claims
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
NP-cIPTG has been used as an optochemical tool for Lac repressor-controlled microbial expression systems.
photo-responsive inducer molecules such as 6-nitropiperonyl-caged IPTG (NP-cIPTG) have been used as optochemical tools for Lac repressor-controlled microbial expression systems
NP-cIPTG has been used as an optochemical tool for Lac repressor-controlled microbial expression systems.
photo-responsive inducer molecules such as 6-nitropiperonyl-caged IPTG (NP-cIPTG) have been used as optochemical tools for Lac repressor-controlled microbial expression systems
NP-cIPTG has been used as an optochemical tool for Lac repressor-controlled microbial expression systems.
photo-responsive inducer molecules such as 6-nitropiperonyl-caged IPTG (NP-cIPTG) have been used as optochemical tools for Lac repressor-controlled microbial expression systems
NP-cIPTG has been used as an optochemical tool for Lac repressor-controlled microbial expression systems.
photo-responsive inducer molecules such as 6-nitropiperonyl-caged IPTG (NP-cIPTG) have been used as optochemical tools for Lac repressor-controlled microbial expression systems
NP-cIPTG has been used as an optochemical tool for Lac repressor-controlled microbial expression systems.
photo-responsive inducer molecules such as 6-nitropiperonyl-caged IPTG (NP-cIPTG) have been used as optochemical tools for Lac repressor-controlled microbial expression systems
NP-cIPTG has been used as an optochemical tool for Lac repressor-controlled microbial expression systems.
photo-responsive inducer molecules such as 6-nitropiperonyl-caged IPTG (NP-cIPTG) have been used as optochemical tools for Lac repressor-controlled microbial expression systems
NP-cIPTG has been used as an optochemical tool for Lac repressor-controlled microbial expression systems.
photo-responsive inducer molecules such as 6-nitropiperonyl-caged IPTG (NP-cIPTG) have been used as optochemical tools for Lac repressor-controlled microbial expression systems
NP-cIPTG has been used as an optochemical tool for Lac repressor-controlled microbial expression systems.
photo-responsive inducer molecules such as 6-nitropiperonyl-caged IPTG (NP-cIPTG) have been used as optochemical tools for Lac repressor-controlled microbial expression systems
Approval Evidence
2 sources2 linked approval claimsfirst-pass slugs 6-nitropiperonyl-np-ciptg, np-ciptg
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Source:
photo-responsive inducer molecules such as 6-nitropiperonyl-caged IPTG (NP-cIPTG) have been used as optochemical tools for Lac repressor-controlled microbial expression systems
Source:
comparative performance claimsupports
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Source:
tool usesupports
NP-cIPTG has been used as an optochemical tool for Lac repressor-controlled microbial expression systems.
photo-responsive inducer molecules such as 6-nitropiperonyl-caged IPTG (NP-cIPTG) have been used as optochemical tools for Lac repressor-controlled microbial expression systems
Source:
Comparisons
Source-stated alternatives
The study used different cIPTG variants, but only NP-cIPTG is singled out in the abstract as especially applicable.; The paper contrasts NP-cIPTG with two newly developed cIPTG derivatives that differ in hydrophobicity and water solubility.
Source:
The study used different cIPTG variants, but only NP-cIPTG is singled out in the abstract as especially applicable.
Source:
The paper contrasts NP-cIPTG with two newly developed cIPTG derivatives that differ in hydrophobicity and water solubility.
Source-backed strengths
The available evidence supports that NP-cIPTG can function as a light-triggered inducer in bacterial LacI-controlled systems. Its application was demonstrated not only for reporter-style gene regulation but also for induction of intrinsic carotenoid biosynthesis in R. capsulatus, indicating utility for engineering cellular function.
Source:
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Compared with alkynyl-functionalized photocleavable linker
NP-cIPTG and alkynyl-functionalized photocleavable linker address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: photocleavage; same primary input modality: light
Strengths here: appears more independently replicated; looks easier to implement in practice.
Compared with Opto-Casp8-V1
NP-cIPTG and Opto-Casp8-V1 address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: photocleavage; same primary input modality: light
Strengths here: appears more independently replicated; looks easier to implement in practice.
Compared with Opto-Casp8-V2
NP-cIPTG and Opto-Casp8-V2 address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: photocleavage; same primary input modality: light
Strengths here: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
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