Source 1primary paper1989Proceedings of the National Academy of Sciences
Toolkit/oat phy-CAT fusion gene
oat phy-CAT fusion gene
Also known as: heterologous oat phy-CAT gene
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The oat phy-CAT fusion gene is a heterologous reporter construct in which the 5'-flanking sequence and part of the structural region of an oat phytochrome gene are fused to chloramphenicol acetyltransferase (CAT). It is used to measure light-regulated transcriptional activity of the oat phy promoter after transient introduction into plant cells.
Usefulness & Problems
Why this is useful
This construct provides a rapid transient assay for testing DNA sequences involved in phytochrome-regulated phy gene expression. The cited study reports that expression from the introduced construct can be assayed in less than 24 hours after particle bombardment.
Problem solved
It addresses the need for a fast experimental system to analyze promoter-level control of phy gene expression by phytochrome. Specifically, it enables assessment of negative feedback regulation in which phytochrome controls transcription of its own phy genes.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Target processes
transcriptionInput: Light
Implementation Constraints
The construct consists of the 5'-flanking sequence and part of the structural region of an oat phy gene fused to the CAT reporter coding sequence. Reported use involved microprojectile-mediated gene transfer by particle bombardment into rice, followed by expression analysis within 24 hours.
The available evidence is limited to a transient particle bombardment assay and does not establish stable expression, quantitative dynamic range, or performance across multiple species or tissues. The supplied evidence also does not define the exact promoter boundaries, light regime dependence beyond phytochrome regulation, or comparative sensitivity relative to other reporters.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug oat-phy-cat-fusion-gene
The 5'-flanking sequence and part of the structural region of an oat phy gene have been fused to a reporter coding sequence (chloramphenicol acetyltransferase, CAT)
Source:
assay timingsupports
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
Source:
biological regulationsupports
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Source:
cross species expression patternsupports
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
Source:
evolutionary conservationsupports
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
Source:
phytochrome regulationsupports
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
Source:
Comparisons
Source-backed strengths
The tool was validated in a microprojectile-mediated transient expression assay after transfer of the oat promoter-reporter construct into rice. Its main reported advantage is rapid readout, with assayable expression in under 24 hours, for studying photoregulated transcriptional control.
Ranked Citations
- 1.