Source 1review2014Current Protocols in Cell Biology
Toolkit/optical dimerizers
optical dimerizers
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Optical dimerizers are genetically encoded actuators that use light to control protein-protein interactions. The cited examples are the CRY2/CIB and UVR8/UVR8 systems, which have been applied to light-regulated transcription, protein localization, and protein secretion.
Usefulness & Problems
Why this is useful
These tools are useful because they provide light-dependent control over protein-protein interactions in genetically encoded formats. The supplied evidence specifically supports their use for regulating transcription, subcellular localization, and secretion with light.
Source:
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
Source:
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
Problem solved
Optical dimerizers address the problem of controlling protein interactions with an external optical input rather than constitutive association. The evidence supports this as a strategy for modulating cellular processes including transcription, protein localization, and protein secretion.
Source:
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
HeterodimerizationTechniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
Implementation requires genetically encoded optical dimerizer components, with CRY2/CIB and UVR8/UVR8 named as example systems. The supplied evidence does not provide construct architecture, illumination parameters, cofactors, or expression and delivery details.
The provided evidence does not report quantitative performance metrics such as dynamic range, kinetics, reversibility, wavelength dependence, or background activity. Independent replication and breadth across organisms or experimental contexts are not established from the supplied material alone.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
CRY2/CIB and UVR8/UVR8 are presented for controlling transcription, protein localization, and protein secretion using light.
We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light.
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
A pulse-controlled LED device is provided for experiments requiring extended light treatments.
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
A pulse-controlled LED device is provided for experiments requiring extended light treatments.
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
A pulse-controlled LED device is provided for experiments requiring extended light treatments.
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
A pulse-controlled LED device is provided for experiments requiring extended light treatments.
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
A pulse-controlled LED device is provided for experiments requiring extended light treatments.
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
A pulse-controlled LED device is provided for experiments requiring extended light treatments.
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
A pulse-controlled LED device is provided for experiments requiring extended light treatments.
we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments
Approval Evidence
1 source3 linked approval claimsfirst-pass slug optical-dimerizers
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
Source:
experimental design considerationsupports
Design of optical dimerizer experiments includes choosing a dimerizer system, photoexcitation sources, and coordinated imaging reporters.
Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters.
Source:
field trendsupports
Numerous new and versatile optical dimerizer systems have been developed in recent years.
In recent years, numerous new and versatile dimerizer systems have been developed.
Source:
review scope summarysupports
Optical dimerizers are genetically encoded actuators that enable light control of protein-protein interactions.
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers'
Source:
Comparisons
Source-backed strengths
A key strength is that these systems are genetically encoded actuators for light control of protein-protein interactions. The cited literature presents multiple application classes—transcription, localization, and secretion—using CRY2/CIB and UVR8/UVR8.
Ranked Citations
- 1.