Source 1primary paper2023
Toolkit/optimized Enhanced Magnet transcription factor
optimized Enhanced Magnet transcription factor
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The optimized Enhanced Magnet transcription factor is a light-responsive split transcription factor developed in Saccharomyces cerevisiae using Enhanced Magnet dimerization modules. It was rationally designed and tested to improve light-sensitive gene expression.
Usefulness & Problems
Why this is useful
This tool is useful for optogenetic control of transcription in yeast through a multi-component, light-responsive transcription factor architecture. The associated study also places it within a modular cloning and laboratory automation workflow for high-throughput construction and characterization of split transcription factors.
Source:
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Problem solved
It addresses the problem of improving light-sensitive gene expression from optogenetic split transcription factors in Saccharomyces cerevisiae. More specifically, the reported optimization targeted performance of an Enhanced Magnet-based transcription factor under light control.
Problem links
Need precise spatiotemporal control with light input
DerivedThe optimized Enhanced Magnet transcription factor is a light-responsive split transcription factor built with Enhanced Magnet dimerization modules and developed in Saccharomyces cerevisiae. It was rationally designed and tested to improve light-sensitive gene expression.
Need tighter control over gene expression timing or amplitude
DerivedThe optimized Enhanced Magnet transcription factor is a light-responsive split transcription factor built with Enhanced Magnet dimerization modules and developed in Saccharomyces cerevisiae. It was rationally designed and tested to improve light-sensitive gene expression.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-induced dimerizationlight-induced dimerizationsplit transcription factor reconstitutionsplit transcription factor reconstitutiontranscriptional activationtranscriptional activationTarget processes
transcriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: multi componentswitch architecture: split
The tool is implemented as a split transcription factor incorporating Enhanced Magnet light-sensitive dimerization modules in Saccharomyces cerevisiae. The source indicates use of a modular cloning scheme and laboratory automation for construction and characterization, but the supplied evidence does not specify construct architecture, promoters, activation domains, or illumination parameters.
The supplied evidence does not report quantitative performance metrics, dynamic range, kinetics, background activity, or wavelength-specific operating details for this optimized construct. Validation is only described in Saccharomyces cerevisiae, and no independent replication is provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
Approval Evidence
1 source1 linked approval claimfirst-pass slug optimized-enhanced-magnet-transcription-factor
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression
Source:
performance improvementsupports
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
Source:
Comparisons
Source-backed strengths
The main reported strength is improved light-sensitive gene expression relative to a non-optimized Enhanced Magnet transcription factor design. It was developed in a system that supports high-throughput construction and characterization in Saccharomyces cerevisiae.
Source:
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
Compared with CRY2-CIB1 light-inducible transcription system
optimized Enhanced Magnet transcription factor and CRY2-CIB1 light-inducible transcription system address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; shared mechanisms: transcriptional activation; same primary input modality: light
Compared with light-controlled Bicoid transcription factor
optimized Enhanced Magnet transcription factor and light-controlled Bicoid transcription factor address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; shared mechanisms: transcriptional activation; same primary input modality: light
Compared with UVB-inducible expression system
optimized Enhanced Magnet transcription factor and UVB-inducible expression system address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; shared mechanisms: split transcription factor reconstitution, transcriptional activation; same primary input modality: light
Ranked Citations
- 1.