Source 1primary paper2017Scientific Reports
Toolkit/opto-Dab1
opto-Dab1
Also known as: single-component, photoactivatable Dab1
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
opto-Dab1 is a single-component, photoactivatable version of Disabled-1 (Dab1) created by exploiting the blue light-sensitive dimerization/oligomerization properties of Arabidopsis thaliana Cryptochrome 2 (Cry2). Upon blue light illumination, it enables rapid, local, and reversible activation of Dab1 downstream signaling.
Usefulness & Problems
Why this is useful
This tool provides optical control over the Reelin-Dab1 signaling pathway with spatial and temporal precision. Source literature indicates that it may be useful for studying biological functions of Reelin-Dab1 signaling in vitro and in vivo.
Source:
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Source:
Here we developed a single-component, photoactivatable Dab1 (opto-Dab1) by using the blue light-sensitive dimerization/oligomerization property of A. thaliana Cryptochrome 2 (Cry2).
Problem solved
opto-Dab1 addresses the need to activate Dab1 signaling rapidly, locally, and reversibly using light rather than conventional pathway stimulation. It also enables experimental control of membrane protrusion, retraction, and ruffling by local illumination in COS7 cells and primary neurons.
Source:
Here we developed a single-component, photoactivatable Dab1 (opto-Dab1) by using the blue light-sensitive dimerization/oligomerization property of A. thaliana Cryptochrome 2 (Cry2).
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
signalingInput: Light
Implementation Constraints
opto-Dab1 is described as a single-component construct based on Dab1 and A. thaliana Cry2, indicating a domain-fusion design that responds to blue light. The available evidence does not specify construct architecture, illumination parameters, expression system details, or cofactor requirements.
The supplied evidence does not report quantitative performance metrics, dynamic range, or comparisons to native Dab1 activation. Although the source suggests possible utility in vivo, the provided evidence does not describe in vivo validation or independent replication.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Opto-Dab1 allows control of membrane protrusion, retraction, and ruffling by local illumination in COS7 cells and primary neurons.
The high spatiotemporal resolution of the opto-Dab1 probe also allows us to control membrane protrusion, retraction and ruffling by local illumination in both COS7 cells and in primary neurons.
Opto-Dab1 allows control of membrane protrusion, retraction, and ruffling by local illumination in COS7 cells and primary neurons.
The high spatiotemporal resolution of the opto-Dab1 probe also allows us to control membrane protrusion, retraction and ruffling by local illumination in both COS7 cells and in primary neurons.
Opto-Dab1 allows control of membrane protrusion, retraction, and ruffling by local illumination in COS7 cells and primary neurons.
The high spatiotemporal resolution of the opto-Dab1 probe also allows us to control membrane protrusion, retraction and ruffling by local illumination in both COS7 cells and in primary neurons.
Opto-Dab1 allows control of membrane protrusion, retraction, and ruffling by local illumination in COS7 cells and primary neurons.
The high spatiotemporal resolution of the opto-Dab1 probe also allows us to control membrane protrusion, retraction and ruffling by local illumination in both COS7 cells and in primary neurons.
Opto-Dab1 allows control of membrane protrusion, retraction, and ruffling by local illumination in COS7 cells and primary neurons.
The high spatiotemporal resolution of the opto-Dab1 probe also allows us to control membrane protrusion, retraction and ruffling by local illumination in both COS7 cells and in primary neurons.
Opto-Dab1 allows control of membrane protrusion, retraction, and ruffling by local illumination in COS7 cells and primary neurons.
The high spatiotemporal resolution of the opto-Dab1 probe also allows us to control membrane protrusion, retraction and ruffling by local illumination in both COS7 cells and in primary neurons.
Opto-Dab1 allows control of membrane protrusion, retraction, and ruffling by local illumination in COS7 cells and primary neurons.
The high spatiotemporal resolution of the opto-Dab1 probe also allows us to control membrane protrusion, retraction and ruffling by local illumination in both COS7 cells and in primary neurons.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 may be useful for studying biological functions of the Reelin-Dab1 signaling pathway in vitro and in vivo.
Opto-Dab1 may be useful to study the biological functions of the Reelin-Dab1 signaling pathway both in vitro and in vivo.
Opto-Dab1 may be useful for studying biological functions of the Reelin-Dab1 signaling pathway in vitro and in vivo.
Opto-Dab1 may be useful to study the biological functions of the Reelin-Dab1 signaling pathway both in vitro and in vivo.
Opto-Dab1 may be useful for studying biological functions of the Reelin-Dab1 signaling pathway in vitro and in vivo.
Opto-Dab1 may be useful to study the biological functions of the Reelin-Dab1 signaling pathway both in vitro and in vivo.
Opto-Dab1 may be useful for studying biological functions of the Reelin-Dab1 signaling pathway in vitro and in vivo.
Opto-Dab1 may be useful to study the biological functions of the Reelin-Dab1 signaling pathway both in vitro and in vivo.
Opto-Dab1 may be useful for studying biological functions of the Reelin-Dab1 signaling pathway in vitro and in vivo.
Opto-Dab1 may be useful to study the biological functions of the Reelin-Dab1 signaling pathway both in vitro and in vivo.
Opto-Dab1 may be useful for studying biological functions of the Reelin-Dab1 signaling pathway in vitro and in vivo.
Opto-Dab1 may be useful to study the biological functions of the Reelin-Dab1 signaling pathway both in vitro and in vivo.
Opto-Dab1 may be useful for studying biological functions of the Reelin-Dab1 signaling pathway in vitro and in vivo.
Opto-Dab1 may be useful to study the biological functions of the Reelin-Dab1 signaling pathway both in vitro and in vivo.
Dab1 activation is sufficient to orient cell movement in the absence of other signals.
This shows that Dab1 activation is sufficient to orient cell movement in the absence of other signals.
Dab1 activation is sufficient to orient cell movement in the absence of other signals.
This shows that Dab1 activation is sufficient to orient cell movement in the absence of other signals.
Dab1 activation is sufficient to orient cell movement in the absence of other signals.
This shows that Dab1 activation is sufficient to orient cell movement in the absence of other signals.
Dab1 activation is sufficient to orient cell movement in the absence of other signals.
This shows that Dab1 activation is sufficient to orient cell movement in the absence of other signals.
Dab1 activation is sufficient to orient cell movement in the absence of other signals.
This shows that Dab1 activation is sufficient to orient cell movement in the absence of other signals.
Dab1 activation is sufficient to orient cell movement in the absence of other signals.
This shows that Dab1 activation is sufficient to orient cell movement in the absence of other signals.
Dab1 activation is sufficient to orient cell movement in the absence of other signals.
This shows that Dab1 activation is sufficient to orient cell movement in the absence of other signals.
The authors developed opto-Dab1, a single-component photoactivatable Dab1 based on the blue light-sensitive dimerization/oligomerization property of Cryptochrome 2.
Here we developed a single-component, photoactivatable Dab1 (opto-Dab1) by using the blue light-sensitive dimerization/oligomerization property of A. thaliana Cryptochrome 2 (Cry2).
The authors developed opto-Dab1, a single-component photoactivatable Dab1 based on the blue light-sensitive dimerization/oligomerization property of Cryptochrome 2.
Here we developed a single-component, photoactivatable Dab1 (opto-Dab1) by using the blue light-sensitive dimerization/oligomerization property of A. thaliana Cryptochrome 2 (Cry2).
The authors developed opto-Dab1, a single-component photoactivatable Dab1 based on the blue light-sensitive dimerization/oligomerization property of Cryptochrome 2.
Here we developed a single-component, photoactivatable Dab1 (opto-Dab1) by using the blue light-sensitive dimerization/oligomerization property of A. thaliana Cryptochrome 2 (Cry2).
The authors developed opto-Dab1, a single-component photoactivatable Dab1 based on the blue light-sensitive dimerization/oligomerization property of Cryptochrome 2.
Here we developed a single-component, photoactivatable Dab1 (opto-Dab1) by using the blue light-sensitive dimerization/oligomerization property of A. thaliana Cryptochrome 2 (Cry2).
The authors developed opto-Dab1, a single-component photoactivatable Dab1 based on the blue light-sensitive dimerization/oligomerization property of Cryptochrome 2.
Here we developed a single-component, photoactivatable Dab1 (opto-Dab1) by using the blue light-sensitive dimerization/oligomerization property of A. thaliana Cryptochrome 2 (Cry2).
The authors developed opto-Dab1, a single-component photoactivatable Dab1 based on the blue light-sensitive dimerization/oligomerization property of Cryptochrome 2.
Here we developed a single-component, photoactivatable Dab1 (opto-Dab1) by using the blue light-sensitive dimerization/oligomerization property of A. thaliana Cryptochrome 2 (Cry2).
The authors developed opto-Dab1, a single-component photoactivatable Dab1 based on the blue light-sensitive dimerization/oligomerization property of Cryptochrome 2.
Here we developed a single-component, photoactivatable Dab1 (opto-Dab1) by using the blue light-sensitive dimerization/oligomerization property of A. thaliana Cryptochrome 2 (Cry2).
Approval Evidence
1 source5 linked approval claimsfirst-pass slug opto-dab1
Here we developed a single-component, photoactivatable Dab1 (opto-Dab1) by using the blue light-sensitive dimerization/oligomerization property of A. thaliana Cryptochrome 2 (Cry2).
Source:
cell behavior controlsupports
Opto-Dab1 allows control of membrane protrusion, retraction, and ruffling by local illumination in COS7 cells and primary neurons.
The high spatiotemporal resolution of the opto-Dab1 probe also allows us to control membrane protrusion, retraction and ruffling by local illumination in both COS7 cells and in primary neurons.
Source:
functional capabilitysupports
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination.
Source:
intended usesupports
Opto-Dab1 may be useful for studying biological functions of the Reelin-Dab1 signaling pathway in vitro and in vivo.
Opto-Dab1 may be useful to study the biological functions of the Reelin-Dab1 signaling pathway both in vitro and in vivo.
Source:
sufficiency claimsupports
Dab1 activation is sufficient to orient cell movement in the absence of other signals.
This shows that Dab1 activation is sufficient to orient cell movement in the absence of other signals.
Source:
tool developmentsupports
The authors developed opto-Dab1, a single-component photoactivatable Dab1 based on the blue light-sensitive dimerization/oligomerization property of Cryptochrome 2.
Here we developed a single-component, photoactivatable Dab1 (opto-Dab1) by using the blue light-sensitive dimerization/oligomerization property of A. thaliana Cryptochrome 2 (Cry2).
Source:
Comparisons
Source-backed strengths
Reported strengths include rapid, local, and reversible activation of downstream signaling upon blue light illumination. The tool was also shown to control membrane protrusion, retraction, and ruffling in both COS7 cells and primary neurons, supporting cell-behavior modulation in multiple in vitro contexts.
Ranked Citations
- 1.