Source 1review2018Chemical Society Reviews
Toolkit/opto-kinases
opto-kinases
Also known as: optogenetic tools for controlling protein kinases
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Opto-kinases are a growing set of optogenetic multi-component tools that use light to activate or inhibit different protein kinases. They are described as tools for regulating cellular signaling with temporal and spatial control.
Usefulness & Problems
Why this is useful
These tools are useful because optogenetic regulation enables fast, reversible, and non-invasive manipulation of protein kinase activities. The literature positions them as optical alternatives or complements to growth factors, protein kinase inhibitors, and chemical dimerizers for controlling signaling.
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Opto-kinases are optogenetic tools that allow activation or inhibition of different protein kinases with light. The review frames them as tools for regulating cellular signaling with spatial and temporal control.
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light-controlled activation of protein kinases
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light-controlled inhibition of protein kinases
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probing spatial aspects of kinase signaling
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probing temporal aspects of kinase signaling
Problem solved
Opto-kinases address the problem of manipulating kinase activity with high temporal and spatial control. The cited review specifically supports rapid, reversible, and non-invasive control of protein kinase activities by light.
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They solve the problem of manipulating kinase activity rapidly, reversibly, and non-invasively. The review presents them as complements to growth factors, kinase inhibitors, and chemical dimerizers.
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enables optical control of kinase activity in cells and organisms
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provides an alternative to chemical or growth-factor-based perturbation of kinase signaling
Published Workflows
Objective: Select or engineer optogenetically controlled protein kinases for light-based regulation of cellular signaling.
Why it works: The review indicates that existing opto-kinase properties can guide tool selection, while photoreceptor-based strategies can be used to engineer new opto-kinases when needed.
light-controlled regulation of kinase activityphotoreceptor-based control of kinase signalingoptogenetic regulationcomparison to chemical regulationphotoreceptor-based engineering
Stages
- 1.Select among existing opto-kinases based on their properties(decision_gate)
The review summarizes properties of existing opto-kinases and presents guidelines for selecting them.
Selection: properties of existing optogenetic tools for controlling tyrosine kinases and serine-threonine kinases
- 2.Select light-control equipment(decision_gate)
The abstract explicitly mentions guidelines for selection of equipment to control opto-kinases with light.
Selection: guidelines for selection of equipment to control opto-kinases with light
- 3.Apply opto-kinases to study signaling in cells and organisms(functional_characterization)
The review discusses how opto-kinases can be applied for studies of spatial and temporal aspects of protein kinase signaling in cells and organisms.
- 4.Engineer novel opto-kinases from photoreceptors(library_design)
When existing tools are insufficient, the review describes strategies to engineer novel opto-kinases from photoreceptors.
Selection: strategies to engineer novel opto-kinases on the basis of various photoreceptors
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
HeterodimerizationTechniques
No technique tags yet.
Target processes
signalingInput: Light
Implementation Constraints
The available evidence indicates that these tools require light and illumination hardware for control. It also states that engineering new versions relies on photoreceptor-based design strategies, but the supplied material does not specify particular photoreceptors, wavelengths, cofactors, or construct architectures.
The supplied evidence is review-level and does not provide tool-specific performance metrics, kinase-by-kinase generality, or direct comparative quantitative data. It also does not support the claim that opto-kinases universally replace chemical regulation methods or work equally well for every kinase.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Optogenetic regulation enables fast, reversible, and non-invasive manipulation of protein kinase activities.
Optogenetic regulation enables fast, reversible, and non-invasive manipulation of protein kinase activities.
Optogenetic regulation enables fast, reversible, and non-invasive manipulation of protein kinase activities.
Optogenetic regulation enables fast, reversible, and non-invasive manipulation of protein kinase activities.
Optogenetic regulation enables fast, reversible, and non-invasive manipulation of protein kinase activities.
Optogenetic regulation enables fast, reversible, and non-invasive manipulation of protein kinase activities.
Optogenetic regulation enables fast, reversible, and non-invasive manipulation of protein kinase activities.
Optogenetic regulation enables fast, reversible, and non-invasive manipulation of protein kinase activities.
Optogenetic regulation enables fast, reversible, and non-invasive manipulation of protein kinase activities.
Opto-kinases can be applied to study spatial and temporal aspects of protein kinase signaling in cells and organisms.
Opto-kinases can be applied to study spatial and temporal aspects of protein kinase signaling in cells and organisms.
Opto-kinases can be applied to study spatial and temporal aspects of protein kinase signaling in cells and organisms.
Opto-kinases can be applied to study spatial and temporal aspects of protein kinase signaling in cells and organisms.
Opto-kinases can be applied to study spatial and temporal aspects of protein kinase signaling in cells and organisms.
Opto-kinases can be applied to study spatial and temporal aspects of protein kinase signaling in cells and organisms.
Opto-kinases can be applied to study spatial and temporal aspects of protein kinase signaling in cells and organisms.
Opto-kinases can be applied to study spatial and temporal aspects of protein kinase signaling in cells and organisms.
Opto-kinases can be applied to study spatial and temporal aspects of protein kinase signaling in cells and organisms.
Opto-kinases allow light-controlled activation and inhibition of different protein kinases.
Opto-kinases allow light-controlled activation and inhibition of different protein kinases.
Opto-kinases allow light-controlled activation and inhibition of different protein kinases.
Opto-kinases allow light-controlled activation and inhibition of different protein kinases.
Opto-kinases allow light-controlled activation and inhibition of different protein kinases.
Opto-kinases allow light-controlled activation and inhibition of different protein kinases.
Opto-kinases allow light-controlled activation and inhibition of different protein kinases.
Opto-kinases allow light-controlled activation and inhibition of different protein kinases.
Opto-kinases allow light-controlled activation and inhibition of different protein kinases.
Optogenetic regulation of protein kinase activity complements traditional methods such as growth factors, protein kinase inhibitors, and chemical dimerizers.
Optogenetic regulation of protein kinase activity complements traditional methods such as growth factors, protein kinase inhibitors, and chemical dimerizers.
Optogenetic regulation of protein kinase activity complements traditional methods such as growth factors, protein kinase inhibitors, and chemical dimerizers.
Optogenetic regulation of protein kinase activity complements traditional methods such as growth factors, protein kinase inhibitors, and chemical dimerizers.
Optogenetic regulation of protein kinase activity complements traditional methods such as growth factors, protein kinase inhibitors, and chemical dimerizers.
Optogenetic regulation of protein kinase activity complements traditional methods such as growth factors, protein kinase inhibitors, and chemical dimerizers.
Optogenetic regulation of protein kinase activity complements traditional methods such as growth factors, protein kinase inhibitors, and chemical dimerizers.
Optogenetic regulation of protein kinase activity complements traditional methods such as growth factors, protein kinase inhibitors, and chemical dimerizers.
Optogenetic regulation of protein kinase activity complements traditional methods such as growth factors, protein kinase inhibitors, and chemical dimerizers.
Novel opto-kinases can be engineered on the basis of various photoreceptors.
Novel opto-kinases can be engineered on the basis of various photoreceptors.
Novel opto-kinases can be engineered on the basis of various photoreceptors.
Novel opto-kinases can be engineered on the basis of various photoreceptors.
Novel opto-kinases can be engineered on the basis of various photoreceptors.
Novel opto-kinases can be engineered on the basis of various photoreceptors.
Novel opto-kinases can be engineered on the basis of various photoreceptors.
Novel opto-kinases can be engineered on the basis of various photoreceptors.
Novel opto-kinases can be engineered on the basis of various photoreceptors.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug opto-kinases
A growing set of optogenetic tools, termed opto-kinases, allows activation and inhibition of different protein kinases with light.
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advantage summarysupports
Optogenetic regulation enables fast, reversible, and non-invasive manipulation of protein kinase activities.
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application scopesupports
Opto-kinases can be applied to study spatial and temporal aspects of protein kinase signaling in cells and organisms.
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capability summarysupports
Opto-kinases allow light-controlled activation and inhibition of different protein kinases.
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comparison summarysupports
Optogenetic regulation of protein kinase activity complements traditional methods such as growth factors, protein kinase inhibitors, and chemical dimerizers.
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Comparisons
Source-backed strengths
The supported advantages are fast, reversible, and non-invasive regulation of kinase activity. The evidence also supports that this tool class can both activate and inhibit different protein kinases with light.
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fast manipulation of protein kinase activities
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reversible manipulation of protein kinase activities
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non-invasive manipulation of protein kinase activities
Ranked Citations
- 1.