Source 1primary paper2015PLoS Biology
Toolkit/Opto-mGluR6
Opto-mGluR6
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Opto-mGluR6 is a chimeric optogenetic receptor designed for retinal ON-bipolar cells. It combines light-sensing domains from melanopsin with intracellular domains from mGluR6 to couple light activation to mGluR6-like GPCR signaling.
Usefulness & Problems
Why this is useful
Opto-mGluR6 is presented as a next-generation, cell-tailored optogenetic tool for retinal ON-bipolar cells. Source claims indicate that it addresses drawbacks of prior optogenetic tools in mouse models, specifically low light sensitivity and limited physiological compatibility.
Source:
We show that Opto-mGluR6 ... reliably recovers vision at the retinal, cortical, and behavioral levels under moderate daylight illumination.
Problem solved
This tool is intended to restore the ON switch in blind retinas by introducing a light-responsive receptor into retinal ON-bipolar cells. The cited literature frames the problem as achieving optogenetic control with better light sensitivity and physiological compatibility than recently developed alternatives.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
chimeric gpcr signalingdomain-coupled signal transductionlight-dependent receptor activationTechniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
Implementation is based on expression of a chimeric protein containing melanopsin-derived light-sensing domains and mGluR6 intracellular domains in retinal ON-bipolar cells. The provided evidence does not specify vector system, promoter, chromophore requirements, or construct architecture in greater detail.
The supplied evidence does not provide quantitative performance data, spectral properties, kinetics, or direct validation details. Independent replication and breadth of testing beyond the cited study are not established from the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Opto-mGluR6 overcomes the low light sensitivity and lack of physiological compatibility described as drawbacks of recently developed optogenetic tools tested in mouse models.
The major drawbacks of all optogenetic tools recently developed and tested in mouse models are their low light sensitivity and lack of physiological compatibility. Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells, which overcomes these limitations.
Opto-mGluR6 overcomes the low light sensitivity and lack of physiological compatibility described as drawbacks of recently developed optogenetic tools tested in mouse models.
The major drawbacks of all optogenetic tools recently developed and tested in mouse models are their low light sensitivity and lack of physiological compatibility. Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells, which overcomes these limitations.
Opto-mGluR6 overcomes the low light sensitivity and lack of physiological compatibility described as drawbacks of recently developed optogenetic tools tested in mouse models.
The major drawbacks of all optogenetic tools recently developed and tested in mouse models are their low light sensitivity and lack of physiological compatibility. Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells, which overcomes these limitations.
Opto-mGluR6 overcomes the low light sensitivity and lack of physiological compatibility described as drawbacks of recently developed optogenetic tools tested in mouse models.
The major drawbacks of all optogenetic tools recently developed and tested in mouse models are their low light sensitivity and lack of physiological compatibility. Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells, which overcomes these limitations.
Opto-mGluR6 overcomes the low light sensitivity and lack of physiological compatibility described as drawbacks of recently developed optogenetic tools tested in mouse models.
The major drawbacks of all optogenetic tools recently developed and tested in mouse models are their low light sensitivity and lack of physiological compatibility. Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells, which overcomes these limitations.
Opto-mGluR6 overcomes the low light sensitivity and lack of physiological compatibility described as drawbacks of recently developed optogenetic tools tested in mouse models.
The major drawbacks of all optogenetic tools recently developed and tested in mouse models are their low light sensitivity and lack of physiological compatibility. Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells, which overcomes these limitations.
Opto-mGluR6 overcomes the low light sensitivity and lack of physiological compatibility described as drawbacks of recently developed optogenetic tools tested in mouse models.
The major drawbacks of all optogenetic tools recently developed and tested in mouse models are their low light sensitivity and lack of physiological compatibility. Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells, which overcomes these limitations.
Opto-mGluR6 is a chimeric protein consisting of intracellular domains of mGluR6 and light-sensing domains of melanopsin.
Opto-mGluR6, a chimeric protein consisting of the intracellular domains of the ON-bipolar cell-specific metabotropic glutamate receptor mGluR6 and the light-sensing domains of melanopsin
Opto-mGluR6 is a chimeric protein consisting of intracellular domains of mGluR6 and light-sensing domains of melanopsin.
Opto-mGluR6, a chimeric protein consisting of the intracellular domains of the ON-bipolar cell-specific metabotropic glutamate receptor mGluR6 and the light-sensing domains of melanopsin
Opto-mGluR6 is a chimeric protein consisting of intracellular domains of mGluR6 and light-sensing domains of melanopsin.
Opto-mGluR6, a chimeric protein consisting of the intracellular domains of the ON-bipolar cell-specific metabotropic glutamate receptor mGluR6 and the light-sensing domains of melanopsin
Opto-mGluR6 is a chimeric protein consisting of intracellular domains of mGluR6 and light-sensing domains of melanopsin.
Opto-mGluR6, a chimeric protein consisting of the intracellular domains of the ON-bipolar cell-specific metabotropic glutamate receptor mGluR6 and the light-sensing domains of melanopsin
Opto-mGluR6 is a chimeric protein consisting of intracellular domains of mGluR6 and light-sensing domains of melanopsin.
Opto-mGluR6, a chimeric protein consisting of the intracellular domains of the ON-bipolar cell-specific metabotropic glutamate receptor mGluR6 and the light-sensing domains of melanopsin
Opto-mGluR6 is a chimeric protein consisting of intracellular domains of mGluR6 and light-sensing domains of melanopsin.
Opto-mGluR6, a chimeric protein consisting of the intracellular domains of the ON-bipolar cell-specific metabotropic glutamate receptor mGluR6 and the light-sensing domains of melanopsin
Opto-mGluR6 is a chimeric protein consisting of intracellular domains of mGluR6 and light-sensing domains of melanopsin.
Opto-mGluR6, a chimeric protein consisting of the intracellular domains of the ON-bipolar cell-specific metabotropic glutamate receptor mGluR6 and the light-sensing domains of melanopsin
Opto-mGluR6 is designed for retinal ON-bipolar cells.
Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells
Opto-mGluR6 is designed for retinal ON-bipolar cells.
Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells
Opto-mGluR6 is designed for retinal ON-bipolar cells.
Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells
Opto-mGluR6 is designed for retinal ON-bipolar cells.
Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells
Opto-mGluR6 is designed for retinal ON-bipolar cells.
Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells
Opto-mGluR6 is designed for retinal ON-bipolar cells.
Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells
Opto-mGluR6 is designed for retinal ON-bipolar cells.
Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells
Opto-mGluR6 reliably recovers vision at retinal, cortical, and behavioral levels under moderate daylight illumination.
We show that Opto-mGluR6 ... reliably recovers vision at the retinal, cortical, and behavioral levels under moderate daylight illumination.
illumination condition moderate daylight illumination
Opto-mGluR6 reliably recovers vision at retinal, cortical, and behavioral levels under moderate daylight illumination.
We show that Opto-mGluR6 ... reliably recovers vision at the retinal, cortical, and behavioral levels under moderate daylight illumination.
illumination condition moderate daylight illumination
Opto-mGluR6 reliably recovers vision at retinal, cortical, and behavioral levels under moderate daylight illumination.
We show that Opto-mGluR6 ... reliably recovers vision at the retinal, cortical, and behavioral levels under moderate daylight illumination.
illumination condition moderate daylight illumination
Opto-mGluR6 reliably recovers vision at retinal, cortical, and behavioral levels under moderate daylight illumination.
We show that Opto-mGluR6 ... reliably recovers vision at the retinal, cortical, and behavioral levels under moderate daylight illumination.
illumination condition moderate daylight illumination
Opto-mGluR6 reliably recovers vision at retinal, cortical, and behavioral levels under moderate daylight illumination.
We show that Opto-mGluR6 ... reliably recovers vision at the retinal, cortical, and behavioral levels under moderate daylight illumination.
illumination condition moderate daylight illumination
Opto-mGluR6 reliably recovers vision at retinal, cortical, and behavioral levels under moderate daylight illumination.
We show that Opto-mGluR6 ... reliably recovers vision at the retinal, cortical, and behavioral levels under moderate daylight illumination.
illumination condition moderate daylight illumination
Opto-mGluR6 reliably recovers vision at retinal, cortical, and behavioral levels under moderate daylight illumination.
We show that Opto-mGluR6 ... reliably recovers vision at the retinal, cortical, and behavioral levels under moderate daylight illumination.
illumination condition moderate daylight illumination
Approval Evidence
1 source4 linked approval claimsfirst-pass slug opto-mglur6
Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells
Source:
comparative advantagesupports
Opto-mGluR6 overcomes the low light sensitivity and lack of physiological compatibility described as drawbacks of recently developed optogenetic tools tested in mouse models.
The major drawbacks of all optogenetic tools recently developed and tested in mouse models are their low light sensitivity and lack of physiological compatibility. Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells, which overcomes these limitations.
Source:
compositionsupports
Opto-mGluR6 is a chimeric protein consisting of intracellular domains of mGluR6 and light-sensing domains of melanopsin.
Opto-mGluR6, a chimeric protein consisting of the intracellular domains of the ON-bipolar cell-specific metabotropic glutamate receptor mGluR6 and the light-sensing domains of melanopsin
Source:
design targetsupports
Opto-mGluR6 is designed for retinal ON-bipolar cells.
Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells
Source:
functional effectsupports
Opto-mGluR6 reliably recovers vision at retinal, cortical, and behavioral levels under moderate daylight illumination.
We show that Opto-mGluR6 ... reliably recovers vision at the retinal, cortical, and behavioral levels under moderate daylight illumination.
Source:
Comparisons
Source-backed strengths
The reported strength is its cell-tailored design for retinal ON-bipolar cells through fusion of melanopsin light-sensing regions with mGluR6 intracellular signaling domains. Source claims further state that it overcomes low light sensitivity and lack of physiological compatibility described for other optogenetic tools tested in mouse models.
Source:
The major drawbacks of all optogenetic tools recently developed and tested in mouse models are their low light sensitivity and lack of physiological compatibility. Here we introduce a next-generation optogenetic tool, Opto-mGluR6, designed for retinal ON-bipolar cells, which overcomes these limitations.
Ranked Citations
- 1.