Source 1primary paper2022
Toolkit/opto-PKR
opto-PKR
Also known as: photo-switchable control over the ISR stress sensor kinase PKR
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
opto-PKR is an engineered photo-switchable version of the integrated stress response sensor kinase PKR that enables light-based virtual control of ISR signaling. In the cited 2022 study, controlled light activation of opto-PKR was used to drive ISR activity and reveal input-proportional transcriptional dynamics.
Usefulness & Problems
Why this is useful
This tool is useful for imposing defined light-controlled inputs onto the integrated stress response through PKR rather than relying on conventional stressors. The cited study used it to interrogate how ISR input dynamics encode information about stress level, duration, and spacing between stress encounters.
Problem solved
opto-PKR addresses the problem of experimentally controlling the ISR with precise, externally specified inputs. It enables virtual activation of the ISR stress sensor kinase PKR to study how signaling input patterns shape downstream transcriptional responses.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-dependent switchingpkr-mediated integrated stress response activationpkr-mediated isr activationTechniques
No technique tags yet.
Target processes
signalingtranscriptionInput: Light
Implementation Constraints
The available evidence indicates that opto-PKR is an engineered optogenetic PKR construct used for light-controlled activation of the ISR. The supplied material does not specify the photosensory module, illumination wavelength, expression system, cofactors, or construct design requirements.
The provided evidence is limited to a single 2022 study and does not include quantitative performance metrics, spectral parameters, construct architecture, or cross-system validation. Independent replication and broader benchmarking against other ISR activation methods are not documented in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The authors engineered opto-PKR to provide photo-switchable, virtual control of the integrated stress response.
we engineered photo-switchable control over the ISR stress sensor kinase PKR (opto-PKR), which allows virtual control of the ISR
The authors engineered opto-PKR to provide photo-switchable, virtual control of the integrated stress response.
we engineered photo-switchable control over the ISR stress sensor kinase PKR (opto-PKR), which allows virtual control of the ISR
The authors engineered opto-PKR to provide photo-switchable, virtual control of the integrated stress response.
we engineered photo-switchable control over the ISR stress sensor kinase PKR (opto-PKR), which allows virtual control of the ISR
The authors engineered opto-PKR to provide photo-switchable, virtual control of the integrated stress response.
we engineered photo-switchable control over the ISR stress sensor kinase PKR (opto-PKR), which allows virtual control of the ISR
The authors engineered opto-PKR to provide photo-switchable, virtual control of the integrated stress response.
we engineered photo-switchable control over the ISR stress sensor kinase PKR (opto-PKR), which allows virtual control of the ISR
The authors engineered opto-PKR to provide photo-switchable, virtual control of the integrated stress response.
we engineered photo-switchable control over the ISR stress sensor kinase PKR (opto-PKR), which allows virtual control of the ISR
The authors engineered opto-PKR to provide photo-switchable, virtual control of the integrated stress response.
we engineered photo-switchable control over the ISR stress sensor kinase PKR (opto-PKR), which allows virtual control of the ISR
ISR input dynamics encode information in stress levels, stress durations, and the timing between stress encounters.
Our results demonstrate that the input dynamics of the ISR encode information in stress levels, durations, and the timing between stress encounters.
ISR input dynamics encode information in stress levels, stress durations, and the timing between stress encounters.
Our results demonstrate that the input dynamics of the ISR encode information in stress levels, durations, and the timing between stress encounters.
ISR input dynamics encode information in stress levels, stress durations, and the timing between stress encounters.
Our results demonstrate that the input dynamics of the ISR encode information in stress levels, durations, and the timing between stress encounters.
ISR input dynamics encode information in stress levels, stress durations, and the timing between stress encounters.
Our results demonstrate that the input dynamics of the ISR encode information in stress levels, durations, and the timing between stress encounters.
ISR input dynamics encode information in stress levels, stress durations, and the timing between stress encounters.
Our results demonstrate that the input dynamics of the ISR encode information in stress levels, durations, and the timing between stress encounters.
ISR input dynamics encode information in stress levels, stress durations, and the timing between stress encounters.
Our results demonstrate that the input dynamics of the ISR encode information in stress levels, durations, and the timing between stress encounters.
ISR input dynamics encode information in stress levels, stress durations, and the timing between stress encounters.
Our results demonstrate that the input dynamics of the ISR encode information in stress levels, durations, and the timing between stress encounters.
Controlled light activation of opto-PKR revealed a biphasic, input-proportional transcriptional response in the ISR with transient and gradual dynamic modes corresponding to adaptive and terminal outcomes.
Using controlled light inputs to activate opto-PKR we traced information flow in the ISR both globally, in the transcriptome, and for key ISR effectors. Our analyses revealed a biphasic, input-proportional transcriptional response with two dynamic modes, transient and gradual, that correspond to adaptive and terminal ISR outcomes.
Controlled light activation of opto-PKR revealed a biphasic, input-proportional transcriptional response in the ISR with transient and gradual dynamic modes corresponding to adaptive and terminal outcomes.
Using controlled light inputs to activate opto-PKR we traced information flow in the ISR both globally, in the transcriptome, and for key ISR effectors. Our analyses revealed a biphasic, input-proportional transcriptional response with two dynamic modes, transient and gradual, that correspond to adaptive and terminal ISR outcomes.
Controlled light activation of opto-PKR revealed a biphasic, input-proportional transcriptional response in the ISR with transient and gradual dynamic modes corresponding to adaptive and terminal outcomes.
Using controlled light inputs to activate opto-PKR we traced information flow in the ISR both globally, in the transcriptome, and for key ISR effectors. Our analyses revealed a biphasic, input-proportional transcriptional response with two dynamic modes, transient and gradual, that correspond to adaptive and terminal ISR outcomes.
Controlled light activation of opto-PKR revealed a biphasic, input-proportional transcriptional response in the ISR with transient and gradual dynamic modes corresponding to adaptive and terminal outcomes.
Using controlled light inputs to activate opto-PKR we traced information flow in the ISR both globally, in the transcriptome, and for key ISR effectors. Our analyses revealed a biphasic, input-proportional transcriptional response with two dynamic modes, transient and gradual, that correspond to adaptive and terminal ISR outcomes.
Controlled light activation of opto-PKR revealed a biphasic, input-proportional transcriptional response in the ISR with transient and gradual dynamic modes corresponding to adaptive and terminal outcomes.
Using controlled light inputs to activate opto-PKR we traced information flow in the ISR both globally, in the transcriptome, and for key ISR effectors. Our analyses revealed a biphasic, input-proportional transcriptional response with two dynamic modes, transient and gradual, that correspond to adaptive and terminal ISR outcomes.
Controlled light activation of opto-PKR revealed a biphasic, input-proportional transcriptional response in the ISR with transient and gradual dynamic modes corresponding to adaptive and terminal outcomes.
Using controlled light inputs to activate opto-PKR we traced information flow in the ISR both globally, in the transcriptome, and for key ISR effectors. Our analyses revealed a biphasic, input-proportional transcriptional response with two dynamic modes, transient and gradual, that correspond to adaptive and terminal ISR outcomes.
Controlled light activation of opto-PKR revealed a biphasic, input-proportional transcriptional response in the ISR with transient and gradual dynamic modes corresponding to adaptive and terminal outcomes.
Using controlled light inputs to activate opto-PKR we traced information flow in the ISR both globally, in the transcriptome, and for key ISR effectors. Our analyses revealed a biphasic, input-proportional transcriptional response with two dynamic modes, transient and gradual, that correspond to adaptive and terminal ISR outcomes.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug opto-pkr
we engineered photo-switchable control over the ISR stress sensor kinase PKR (opto-PKR), which allows virtual control of the ISR
Source:
engineering resultsupports
The authors engineered opto-PKR to provide photo-switchable, virtual control of the integrated stress response.
we engineered photo-switchable control over the ISR stress sensor kinase PKR (opto-PKR), which allows virtual control of the ISR
Source:
information encodingsupports
ISR input dynamics encode information in stress levels, stress durations, and the timing between stress encounters.
Our results demonstrate that the input dynamics of the ISR encode information in stress levels, durations, and the timing between stress encounters.
Source:
observed response patternsupports
Controlled light activation of opto-PKR revealed a biphasic, input-proportional transcriptional response in the ISR with transient and gradual dynamic modes corresponding to adaptive and terminal outcomes.
Using controlled light inputs to activate opto-PKR we traced information flow in the ISR both globally, in the transcriptome, and for key ISR effectors. Our analyses revealed a biphasic, input-proportional transcriptional response with two dynamic modes, transient and gradual, that correspond to adaptive and terminal ISR outcomes.
Source:
Comparisons
Source-backed strengths
The reported strength of opto-PKR is photo-switchable control over PKR-dependent ISR activation. In the cited work, controlled light activation revealed a biphasic, input-proportional transcriptional response with transient and gradual dynamic modes associated with adaptive and terminal outcomes.
Source:
we engineered photo-switchable control over the ISR stress sensor kinase PKR (opto-PKR), which allows virtual control of the ISR
Ranked Citations
- 1.